Efficiency of azithromycin and tobramycin drug combination on Pseudomonas aeruginosa

doi:10.3969/j.issn.1673-8640.2017.010.010

Abstract

Abstract:

Objective To investigate the inhibitory effect of azithromycin（AZM） and tobramycin（TOB） drug combination on Pseudomonas aeruginosa and biofilm. Methods Chequer board dilution method was used to determine the inhibitory activity of AZM and TOB drug combination on Pseudomonas aeruginosastandard isolate（PAO1）and clinical isolates（PA0121 and PA1324） in planktonic form. The combined method of 96-well plates with crystal violet staining was used to determine the inhibitory effect of AZM and TOB drug combination on Pseudomonas aeruginosa biofilm formation. Results The fractional inhibitory concentration（FIC） index of the 3 isolates was 2. However,1 μg/mL AZM could enhance the inhibitory effect of 0.25,0.5 and 1 μg/mL TOB on PAO1 biofilm formation（P<0.05）. The synergistic effect was obvious at AZM level 2 μg/mL with inhibitory effect on PA0121 and PA1324 biofilm formation（P<0.05）. Conclusions There is a synergistic effect of AZM and TOB drug combination on Pseudomonas aeruginosa in biofilm formation but independent effect in planktonic form.

Key words: Azithromycin, Tobramycin, Pseudomonas aeruginosa, Biofilm, Synergistic effect, Drug combination

CLC Number:

R446.5

FAN Xuemei. Efficiency of azithromycin and tobramycin drug combination on Pseudomonas aeruginosa[J]. Laboratory Medicine, 2017, 32(10): 879-882.

Figures/Tables 3

References 11

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菌株	MIC（μg/mL）				FIC	结果判断
	单用		联合应用
	AZM	TOB	AZM	TOB
PAO1	256	2.0	256	2.0	2.0	无关
PA0121	128	0.5	128	0.5	2.0	无关
PA1324	256	0.5	256	0.5	2.0	无关

菌株	MIC（μg/mL）				FIC	结果判断
	单用		联合应用
	AZM	TOB	AZM	TOB
PAO1	256	2.0	256	2.0	2.0	无关
PA0121	128	0.5	128	0.5	2.0	无关
PA1324	256	0.5	256	0.5	2.0	无关

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