Abstract:

Objective To establish a multiplex polymerase chain reaction （PCR） system to detect common bacteria in children with bloodstream infection. Methods A total of 7 kinds of common bacteria were screened out based on the results of 16S rDNA-PCR and sequencing,and then the special primers were designed to establish a multiplex PCR system. The determination performance was evaluated by using 15 bacteria,4 fungi and human cells. The results of multiplex PCR system were compared with those of blood culture and 16S rDNA-PCR. Results The positive rate of blood culture （14 cases）, 16S rDNA-PCR （28 cases） and multiplex PCR system （25 cases） were 7.8%,15.6% and 13.9%, respectively. This multiplex PCR system had a higher detection rate than blood culture（P<0.05） ,and there was no statistical significance compared with 16S rDNA-PCR（P>0.05）. Conclusions The established multiplex PCR system exhibits high speed and accuracy for the detection of 7 kinds of common bacteria,which is earlier for 3-5 d than blood culture in identifying bacterium species,and it can guide clinicians for rational antibacterial agent usage in time.

Key words: Multiplex polymerase chain reaction, Bloodstream infection, Bacterium, Child