Abstract:

Objective To study the relationship between single nucleotide polymorphism (SNP) in chromosome 9p21 and the susceptibility of cerebral infarction. Methods Polymerase chain reaction-ligase detection reaction (PCR-LDR) was applied to determine the six gene locus of SNP between the observation group and normal control group. These six gene locus were rs10757278, rs1333049, rs2383206, rs1537378, rs4977574 and rs2383207 in chromosome 9p21.3. And the observation group had 528 cases of patients with non cardiogenic cerebral infarction (375 cases of them had carotid artery plaque, and the other 153 cases did not have), while the normal control group had 258 cases. Using multivariate Logistic regression and χ² test to analysis the site of the statistical significant genetic interactions, calculated the odds ratios (OR) and 95% confidence interval (CI). Results At the age of 45-65 middle-aged crowd, G allelic genes of rs2383206 and rs4977574, the carriers without carotid artery plaque in cerebral infarction group (55.0% and 54.2%) were significantly more than the normal control group (45.9% and 44.4%, P=0.024, 0.015);And to the risk of cerebral infarction, the GG genotype homozygous mutations accompanied with two gene locus rs2383206 and rs4977574 was 1.733 times to non-GG genotype homozygous mutations(GA+AA). The six gene locus had strong disequilibrium between relations. Conclusions 9p21 chromosome zone in rs2383206 and rs4977574 two loci gene SNP relate to the susceptibility to the occurrence of acute cerebral infarction.

Key words: Single nucleotide polymorphism, Chromosome 9p21, Cerebral infarction