Correlation of ApoH gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke

doi:10.3969/j.issn.1673-8640.2017.12.004

Abstract

Abstract:

Objective To study the correlation of apopoprotein H（ApoH） gene single nucleotide polymorphisms（SNP）and carotid plaques in patients with ischemic cerebral stroke（ICS）. Methods A total of 737 patients with ICS were enrolled from Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine and Department of Neurology of Central Hospital of Shanghai Minhang District. They were classified into carotid plaque group（431 cases） and non-carotid plaque group（306 cases）（control group）. By multiple polymerase chain reaction（PCR） and sequence analysis,the genotype and allele frequencies of ApoH gene SNP loci（rs8178842,rs6933,rs4791079,rs2873966 and rs1801690） between the 2 groups were compared,and the relative risk was evaluated. Results In carotid plaque group,triglyceride（TG）,total cholesterol（TC）,low-density lipoprotein cholesterol（LDL-C）,non-high-density lipoprotein cholesterol（non-HDL-C）,systolic blood pressure and blood glucose were higher than those in control group（P<0.05）. In carotid plaque group,rs4791079 locus CA and CA/AA genotype frequencies were 15.3% and 16.5%,which were higher than those in control group（8.8% and 8.8%）（P=0.008,P=0.022）. In carotid plaque group,rs1801690 locus GC/CC genotype frequency was 8.8%,which was lower than that in control group（13.4%）（P=0.049）. In carotid plaque group,rs4791079 locus A allele frequency（8.8%） was higher than that in control group（4.4%）（P=0.001）. The relative risk analysis found that carrying A allele increased the risk of carotid plaque [odds ratio（OR）=2.095,95% confidence interval（CI）1.333-3.292]. In carotid plaque group,rs1801690 locus C allele frequency（4.4%）was lower than that in control group（7.0%）（P=0.030）. The relative risk analysis found that carrying C allele decreased the risk of carotid plaque（OR=0.610,95%CI 0.389-0.956）. Conclusions ApoH gene rs4791079 and rs1801690 loci have correlations with carotid plaques in patients with ICS.

Key words: Apopoprotein H, Ischemic cerebral stroke, Single nucleotide polymorphism, Genotype frequency, Carotid plaque

CLC Number:

R446.1

SHU Ming, WANG Yan, CHEN Ning, WU Jiemin, NI Peihua. Correlation of ApoH gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke[J]. Laboratory Medicine, 2017, 32(12): 1089-1094.

Figures/Tables 6

References 14

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SNP位点	上游引物	下游引物	长度（bp）
rs2873966	GCCAGTTGGTGATGAATCCT	TGCAAAAACCAACCACACAT	269
rs1801690	CTCCCCCAACAGAACACAGT	TGCCAACTTCACAGCCATAG	274
rs6933	CTCCCCCAACAGAACACAGT	TGCCAACTTCACAGCCATAG	274
rs4791079	TCACCCCAAGTGACAAAACA	TAAACGCTGGCCATTACACA	192
rs8178842	CCTGGGCAACAAGAGTGAA	GAGCTGTGATCACGCCACT	292

SNP位点	上游引物	下游引物	长度（bp）
rs2873966	GCCAGTTGGTGATGAATCCT	TGCAAAAACCAACCACACAT	269
rs1801690	CTCCCCCAACAGAACACAGT	TGCCAACTTCACAGCCATAG	274
rs6933	CTCCCCCAACAGAACACAGT	TGCCAACTTCACAGCCATAG	274
rs4791079	TCACCCCAAGTGACAAAACA	TAAACGCTGGCCATTACACA	192
rs8178842	CCTGGGCAACAAGAGTGAA	GAGCTGTGATCACGCCACT	292

SNP位点	探针序列	长度（bp）
rs2873966_modify	P-GAAAAAGTAGGAATAGTTCCTTTTTTTTTTTTTTTTTT-FAM
rs2873966_C	TTTTTTTTTTTTTTTTATGGGAGAGACCATTGAGATACG	77
rs2873966_T	TTTTTTTTTTTTTTTTTTATGGGAGAGACCATTGAGATACA	79
rs1801690_modify	P-AAAAAGCCAGAGAACTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs1801690_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTACATCGGATGCATCAGTTTTCG	97
rs1801690_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTACATCGGATGCATCAGTTTTCC	99
rs6933_modify	P-AAAATGTCACACTTGTTTCTTGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTTTTTTTTTTTTTTT-FAM
rs6933_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT- CTAAGGTGGTTTTCAGATTCCACAT	166
rs6933_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTCTAAGGTGGTTTTCAGATTCCACAC	168
rs4791079_modify	P-GAGAAAGGGGAAATGTTAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTTTTTTTTTTTTTTTT-FAM
rs4791079_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTACTTTTCAAACTCTACGAATTGCTT	170
rs4791079_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTACTTTTCAAACTCTACGAATTGCTG	172
rs8178842_modify	P-TTCTTAAACAATTATTTATTTGAAATTTTTTTTTTTTTTTTTTTTTTTTGCATCACTCA-FAM
rs8178842_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTTTCGTTTTTGCTTGTTCAGGTT	87
rs8178842_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTTTCGTTTTTGCTTGTTCAGGTC	89

SNP位点	探针序列	长度（bp）
rs2873966_modify	P-GAAAAAGTAGGAATAGTTCCTTTTTTTTTTTTTTTTTT-FAM
rs2873966_C	TTTTTTTTTTTTTTTTATGGGAGAGACCATTGAGATACG	77
rs2873966_T	TTTTTTTTTTTTTTTTTTATGGGAGAGACCATTGAGATACA	79
rs1801690_modify	P-AAAAAGCCAGAGAACTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs1801690_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTACATCGGATGCATCAGTTTTCG	97
rs1801690_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTACATCGGATGCATCAGTTTTCC	99
rs6933_modify	P-AAAATGTCACACTTGTTTCTTGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTTTTTTTTTTTTTTT-FAM
rs6933_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT- CTAAGGTGGTTTTCAGATTCCACAT	166
rs6933_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTCTAAGGTGGTTTTCAGATTCCACAC	168
rs4791079_modify	P-GAGAAAGGGGAAATGTTAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTTTTTTTTTTTTTTTT-FAM
rs4791079_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTACTTTTCAAACTCTACGAATTGCTT	170
rs4791079_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTACTTTTCAAACTCTACGAATTGCTG	172
rs8178842_modify	P-TTCTTAAACAATTATTTATTTGAAATTTTTTTTTTTTTTTTTTTTTTTTGCATCACTCA-FAM
rs8178842_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTTTCGTTTTTGCTTGTTCAGGTT	87
rs8178842_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTTTCGTTTTTGCTTGTTCAGGTC	89

SNP位点	对照组（306例）		斑块组（431例）
SNP位点	统计值	P值	统计值	P值
rs8178842	0.787 1	0.375 0	0.967 8	0.325 2
rs2873966	0.028 1	0.866 8	0.070 9	0.790 1
rs4791079	0.651 8	0.419 5	0.976 8	0.323 0
rs6933	1.058 8	0.303 5	0.005 4	0.941 3
rs1801690	0.183 4	0.668 5	0.916 6	0.338 4