Abstract:

Objective To explore the association between the rs6056 single nucleotide polymorphism (SNP) of fibrinogen (FIB) gene distribution rate and gentic predisposition with diabetic foot and the influence on functional expression of plasma FIB. Methods Using the polymerase chain reaction-ligation detection reaction (PCR-LDR) method, rs6056 polymorphism of FIB gene was studied in 123 patients with diabetic foot (DF group), 97 patients with type 2 diabetes mellitus (T2DM group) and 80 controls (control group). Serum samples were collected at the recruit time for the determination of fasting blood glucose (FPG), 2 hour postprandial blood glucose (2 hPG), white blood cell (WBC) count, FIB and other indicators. Results The levels of WBC and FIB in DF group were significantly higher than those in T2DM group and control group (P<0.05). The diabetic duration of DF group was longer than that of T2DM group. Age and gender had no significant difference among three groups (P>0.05). The serum FIB and WBC of Wagner Ⅴgrade group were significantly higher than those of Wagner Ⅳ grade group and Wagner 0~Ⅲ grade group (P<0.05). Serum FIB of TT genotype was significantly higher than CT and CC genotype in DF group (P<0.05). The serum levels of FIB of TT and CT genotype were significantly higher than those of CC gerotype in Wagner 0~Ⅲ grade group (P<0.05). The serum FIB of TT genotype was significantly higher than CT and CC genotype in Wagner Ⅳgrade group (P<0.05). The serum levels of FIB had no significant difference among three genotypes with WagnerⅤgrade group (P>0.05). Univariate Logistic regression analysis showed that WBC, FIB, CT genotype, TT genotype and T allele were risk factors for DF occurrence. Multivariate Logistic regression analysis showed that TT genotype and T allele were not independent risk factors of diabetic foot, after it was adjusted for WBC and FIB. Conclusions TT genotype is associated with the level of FIB. The FIB gene rs6056 polymorphism may relate to the occurrence and development of diabetic foot.

Key words: Fibrinogen, Single nucleotide polymorphism, rs6056, Diabetic foot