By retrospective investigation from January 1, 2011 to December 31, 2013, the bacteria isolated from blood specimens were collected for analysis.

A total of 2 418 blood culture specimens were detected, there were 226 isolates of pathogenic bacteria, and the positive rate was 9.3%, including 143 isolates of gram-negative bacilli (63.3%), 83 isolates of gram-positive cocci (36.7%). The biggest isolation was Escherichia coli (99 isolates, 43.8%), 43 isolates of coagulase-negative Staphylococcus (19.0%) and 20 isolates of Klebsiella pneumoniae (8.8%). Escherichia coli and Klebsiella pneumoniae were sensitive to imipenem, amikacin and piperacillin-tazobactam. Gram-positive coccus is highly sensitive to vancomycin and teicoplanin.

The main isolated blood culture pathogenic bacterium is gram-negative bacillus, and the drug resistance of isolated pathogenic bacterium is severe. The clinical pathogenic bacterium resistance monitoring and the rational use of antimicrobial agents should be strengthened.