The phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa by modified imipenem-EDTA disk potentiation test

doi:10.3969/j.issn.1673-8640.2014.01.016

Abstract

Abstract: Objective To evaluate the modified imipenem-ethylene diamine tetraacetic acid (EDTA) disk potentiation test for the phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa(PA). Methods A total of 95 carbapenem-resistant PA were collected. The minimum inhibition concentrations(MIC) of imipenem and meropenem were determined by agar dilution method.Polymerase chain reaction (PCR) and DNA sequencing were used for the identification of metallo-beta-lactamase genotypes. The phenotypic detection of metallo-beta-lactamase was done by conventional and modified imipenem-EDTA disk potentiation test. Results The resistance rates of imipenem and meropenem were 100.0% and 71.6% among these 95 carbapenem-resistant PA. The sensitivity, specificity, positive predictive value and negative predictive value of conventional test were 100.0%, 96.6%, 72.7% and 100.0%, respectively. Those of modified test were all 100.0%. Results The modified imipenem-EDTA disk potentiation test could be an effective method for the phenotypic detection of metallo-beta-lactamase-producing PA.

Key words: Metallo-beta-lactamase, Pseudomonas aeruginosa, Disk potentiation test

CLC Number:

R446.5

YAN Yuzhong, FAN Huiqing, ZHENG Wenlong, YANG Huanzhang, LU Yanchun, SHI Yi.. The phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa by modified imipenem-EDTA disk potentiation test[J]. , 2014, 29(1): 65-68.

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