Abstract: Objective　To analyze the antibiotic susceptibility of metallo-beta-lactamase (MBL) of imipenem- resistant Pseudomonas aeruginosa(PA)，and provide the reference for nosocomial infection prevention. Methods　A total of 460 PA were isolated and collected from 2009 to 2011，and the imipenem-resistant isolates were screened. With the combination of disk diffusion method，the MBL phenotype of imipenem-resistant isolates was detected. The MBL genotype was detected by polymerase chain reaction (PCR). The positive isolates amplified by PCR were sequenced. The antibiotic resistance of MBL-producing isolates of imipenem-resistant PA was compared with that of non MBL-producing isolates. The antibiotic resistance characteristics of the different genotypes of MBL-producing isolates were analyzed. Results　In 460 PA，28 MBL-producing isolates were identified. The 19 isolates were IMP-1 type，and 2 isolates were VIM-2 type by PCR. The 1 isolate of IMP-1 type and 1 isolate of VIM-2 type were selected randomly and sequenced. GenBank accessions were No. AY1686359 and AF191564，respectively. The resistance rate to meropenem of MBL-producing isolates was 95.2%，which was higher than that of non MBL-producing isolates(73.7%， P＜0.05). The resistance rates to other antibiotics of MBL-producing isolates were higher than those of non MBL-producing isolates，but the difference was not statistically significant (P＞0.05). The genotypes of MBL-producing isolates in imipenem-resistant PA were different，and the antibiotic resistances were also different. Conclusions　The main genotype of MBL-producing isolates in PA is IMP-1 type. These isolates have strong antibiotic resistance to a lot of antibiotics commonly used in clinic. The epidemiology surveillance of the MBL-producing isolates must be strengthened to prevent and control the resistance spread. Simultaneously，clinical antibiotics should be used according to the antibiotic susceptibility tests.

Key words: Pseudomonas aeruginosa, Metallo-beta-lactamase, Antibiotic resistance analysis