ERIC-PCR typing and main drug resistance mechanism in 75 isolates of carbapenem-resistant Pseudomonas aeruginosa

doi:10.3969/j.issn.1673-8640.2014.07.015

Abstract

Abstract:

Objective To investigate the homology and main drug resistance mechanism of carbapenem-resistant Pseudomonas aeruginosa. Methods　A total of 946 samples were collected， and carbapenem-resistant Pseudomonas aeruginosa were isolated. The drug sensitivity of carbapenem-resistant Pseudomonas aeruginosa were detected by agar dilution test. Enterobacterial repetitive intergenic consensus-polymerase chain reaction（ERIC-PCR） was performed to analyze homology. The isolates were screened by metallo-beta-lactamae. The efflux pump of Pseudomonas aeruginosa resistant to carbapenem antibiotics was detected by efflux pump inhibitor test. Results　A total of 75 isolates of carbapenem-resistant Pseudomonas aeruginosa were isolated. The ERIC-PCR homology analysis showed 8 types， in which A-type and B-type were the main types， accounting for 34.7%（26 isolates） and 22.7%（17 isolates）， respectively. In the 75 isolates resistant to carbapenems， 13 isolates were positive in metal enzyme test， accounting for 17.3%. MC207110 efflux pump inhibitor could make the minimal inhibitory concentration（MIC） of meropenem in 34 isolates being resistant to meropenem decreasing by 4 times or more， compared with single drug therapy， accounting for 63.0%. Conclusions　There are mainly 2 kinds of cloning existing in Pseudomonas aeruginosa being resistant to carbapenem. The production of metallo-beta-lactamase and multi-drug resistant efflux system play important roles in the drug resistance of Pseudomonas aeruginosa.

Key words: Pseudomonas aeruginosa, Carbapenem-resistance, Enterobacterial repetitive intergenic consensus-polymerase chain reaction, Beta-lactamase, Efflux pump

CLC Number:

R446.5

ZHANG Yong,LIU Aisheng,WEN Yan. ERIC-PCR typing and main drug resistance mechanism in 75 isolates of carbapenem-resistant Pseudomonas aeruginosa[J]. , 2014, 29(7): 755-759.

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