Laboratory Medicine ›› 2015, Vol. 30 ›› Issue (9): 939-943.DOI: 10.3969/j.issn.1673-8640.2015.09.017
• Orginal Article • Previous Articles Next Articles
PAN Jun, XU Qingxia, XIAO Weiqiang, CHANG Yanmin, SHEN Yong
Received:
2014-10-08
Online:
2015-09-30
Published:
2015-09-29
CLC Number:
PAN Jun, XU Qingxia, XIAO Weiqiang, CHANG Yanmin, SHEN Yong. Multilocus sequence typing study of ESBLs-producing Escherichia coli in one hospital[J]. Laboratory Medicine, 2015, 30(9): 939-943.
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URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2015.09.017
管家基因 | 引物序列(5'-3') | 长度(bp) | 退火温度(℃) |
---|---|---|---|
adk | F:ATTCTGCTTGGCGCTCCGGG | 583 | 54 |
R:CCGTCAACTTTCGCGTATTT | |||
fumC | F:TCACAGGTCGCCAGCGCTTC | 806 | 54 |
R:GTACGCAGCGAAAAAGATTC | |||
gyrB | F:TCGGCGACACGGATGACGGC | 911 | 60 |
R:ATCAGGCCTTCACGCGCATC | |||
icd | F:ATGGAAAGTAAAGTAGTTGTTCCGGCACA | 878 | 54 |
R:GGACGCAGCAGGATCTGTT | |||
mdh | F:ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG | 932 | 60 |
R:TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT | |||
purA | F:CGCGCTGATGAAAGAGATGA | 816 | 54 |
R:CATACGGTAAGCCACGCAGA | |||
recA | F:CGCATTCGCTTTACCCTGACC | 780 | 58 |
R:TCGTCGAAATCTACGGACCGGA |
管家基因 | 引物序列(5'-3') | 长度(bp) | 退火温度(℃) |
---|---|---|---|
adk | F:ATTCTGCTTGGCGCTCCGGG | 583 | 54 |
R:CCGTCAACTTTCGCGTATTT | |||
fumC | F:TCACAGGTCGCCAGCGCTTC | 806 | 54 |
R:GTACGCAGCGAAAAAGATTC | |||
gyrB | F:TCGGCGACACGGATGACGGC | 911 | 60 |
R:ATCAGGCCTTCACGCGCATC | |||
icd | F:ATGGAAAGTAAAGTAGTTGTTCCGGCACA | 878 | 54 |
R:GGACGCAGCAGGATCTGTT | |||
mdh | F:ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG | 932 | 60 |
R:TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT | |||
purA | F:CGCGCTGATGAAAGAGATGA | 816 | 54 |
R:CATACGGTAAGCCACGCAGA | |||
recA | F:CGCATTCGCTTTACCCTGACC | 780 | 58 |
R:TCGTCGAAATCTACGGACCGGA |
菌株 编号 | 样本种类 | 科室 | 管家基因 | ST | ||||||
---|---|---|---|---|---|---|---|---|---|---|
adk | fumC | gyrB | icd | mdh | purA | recA | ||||
1 | 引流液 | 肝胆一 | 20 | 45 | 41 | 43 | 5 | 32 | 2 | ST117 |
2 | 引流液 | 肝胆一 | 85 | 88 | 78 | 29 | 59 | 58 | 62 | ST354 |
3 | 血液 | 肝胆一 | 9 | 65 | 5 | 18 | 11 | 8 | 6 | ST2179 |
4 | 血液 | 肝胆二 | 35 | 37 | 29 | 25 | 4 | 5 | 73 | ST405 |
5 | 痰液 | 淋巴综合 | 10 | 11 | 4 | 8 | 8 | 13 | 2 | ST167 |
6 | 痰液 | 胸二 | 4 | 26 | 2 | 25 | 4 | 5 | 19 | ST2003 |
7 | 痰液 | 胸二 | 53 | 40 | 47 | 13 | 36 | 28 | 29 | ST131 |
8 | 痰液 | 胸三 | 21 | 35 | 27 | 6 | 5 | 5 | 4 | ST69 |
9 | 穿刺液 | 肝胆二 | 9 | 6 | 15 | 56 | 11 | 26 | 6 | ST2178 |
10 | 引流液 | 肝胆三 | 53 | 40 | 47 | 13 | 17 | 28 | 29 | ST3483 |
11 | 血液 | 普三 | 4 | 26 | 2 | 25 | 5 | 5 | 19 | ST38 |
12 | 脓液 | 普六 | 14 | 14 | 10 | 200 | 17 | 7 | 10 | ST1193 |
13 | 引流液 | 普五 | 4 | 26 | 2 | 25 | 5 | 5 | 19 | ST38 |
14 | 血液 | 普五 | 101 | 88 | 97 | 108 | 26 | 79 | 2 | ST457 |
15 | 中段尿 | 普五 | 10 | 11 | 4 | 8 | 8 | 8 | 2 | ST10 |
16 | 引流液 | 普一 | 21 | 35 | 27 | 6 | 5 | 5 | 4 | ST69 |
17 | 腹水 | 重症监护病房 | 92 | 4 | 87 | 96 | 70 | 58 | 2 | ST648 |
菌株 编号 | 样本种类 | 科室 | 管家基因 | ST | ||||||
---|---|---|---|---|---|---|---|---|---|---|
adk | fumC | gyrB | icd | mdh | purA | recA | ||||
1 | 引流液 | 肝胆一 | 20 | 45 | 41 | 43 | 5 | 32 | 2 | ST117 |
2 | 引流液 | 肝胆一 | 85 | 88 | 78 | 29 | 59 | 58 | 62 | ST354 |
3 | 血液 | 肝胆一 | 9 | 65 | 5 | 18 | 11 | 8 | 6 | ST2179 |
4 | 血液 | 肝胆二 | 35 | 37 | 29 | 25 | 4 | 5 | 73 | ST405 |
5 | 痰液 | 淋巴综合 | 10 | 11 | 4 | 8 | 8 | 13 | 2 | ST167 |
6 | 痰液 | 胸二 | 4 | 26 | 2 | 25 | 4 | 5 | 19 | ST2003 |
7 | 痰液 | 胸二 | 53 | 40 | 47 | 13 | 36 | 28 | 29 | ST131 |
8 | 痰液 | 胸三 | 21 | 35 | 27 | 6 | 5 | 5 | 4 | ST69 |
9 | 穿刺液 | 肝胆二 | 9 | 6 | 15 | 56 | 11 | 26 | 6 | ST2178 |
10 | 引流液 | 肝胆三 | 53 | 40 | 47 | 13 | 17 | 28 | 29 | ST3483 |
11 | 血液 | 普三 | 4 | 26 | 2 | 25 | 5 | 5 | 19 | ST38 |
12 | 脓液 | 普六 | 14 | 14 | 10 | 200 | 17 | 7 | 10 | ST1193 |
13 | 引流液 | 普五 | 4 | 26 | 2 | 25 | 5 | 5 | 19 | ST38 |
14 | 血液 | 普五 | 101 | 88 | 97 | 108 | 26 | 79 | 2 | ST457 |
15 | 中段尿 | 普五 | 10 | 11 | 4 | 8 | 8 | 8 | 2 | ST10 |
16 | 引流液 | 普一 | 21 | 35 | 27 | 6 | 5 | 5 | 4 | ST69 |
17 | 腹水 | 重症监护病房 | 92 | 4 | 87 | 96 | 70 | 58 | 2 | ST648 |
菌株编号 | 样本种类 | 科室 | 管家基因 | ST | ||||||
---|---|---|---|---|---|---|---|---|---|---|
adk | fumC | gyrB | icd | mdh | purA | recA | ||||
1 | 大便 | 肝胆一 | 35 | 37 | 29 | 25 | 4 | 5 | 73 | ST405 |
2 | 大便 | 肝胆一 | 9 | 65 | 5 | 18 | 11 | 8 | 6 | ST2179 |
3 | 大便 | 肝胆二 | 21 | 35 | 27 | 6 | 5 | 5 | 4 | ST69 |
4 | 患者手 | 淋巴综合 | 10 | 11 | 4 | 8 | 8 | 13 | 2 | ST167 |
5 | 陪护手 | 肝胆二 | 6 | 4 | 12 | 1 | 20 | 18 | 7 | ST410 |
6 | 患者手 | 胸二 | 4 | 26 | 2 | 25 | 4 | 5 | 19 | ST2003 |
7 | 床把手 | 普五 | 10 | 11 | 4 | 8 | 8 | 8 | 2 | ST10 |
8 | 床头柜 | 胸二 | 53 | 40 | 47 | 13 | 36 | 28 | 29 | ST131 |
9 | 门 | 胸二 | 53 | 40 | 47 | 13 | 36 | 28 | 29 | ST131 |
菌株编号 | 样本种类 | 科室 | 管家基因 | ST | ||||||
---|---|---|---|---|---|---|---|---|---|---|
adk | fumC | gyrB | icd | mdh | purA | recA | ||||
1 | 大便 | 肝胆一 | 35 | 37 | 29 | 25 | 4 | 5 | 73 | ST405 |
2 | 大便 | 肝胆一 | 9 | 65 | 5 | 18 | 11 | 8 | 6 | ST2179 |
3 | 大便 | 肝胆二 | 21 | 35 | 27 | 6 | 5 | 5 | 4 | ST69 |
4 | 患者手 | 淋巴综合 | 10 | 11 | 4 | 8 | 8 | 13 | 2 | ST167 |
5 | 陪护手 | 肝胆二 | 6 | 4 | 12 | 1 | 20 | 18 | 7 | ST410 |
6 | 患者手 | 胸二 | 4 | 26 | 2 | 25 | 4 | 5 | 19 | ST2003 |
7 | 床把手 | 普五 | 10 | 11 | 4 | 8 | 8 | 8 | 2 | ST10 |
8 | 床头柜 | 胸二 | 53 | 40 | 47 | 13 | 36 | 28 | 29 | ST131 |
9 | 门 | 胸二 | 53 | 40 | 47 | 13 | 36 | 28 | 29 | ST131 |
[1] | 彭乃杰,林哈妮,夏菲,等. 碳青霉稀类耐药肺炎克雷伯菌耐药机制和多位点序列分型研究[J].检验医学,2013,28(9): 862-863. |
[2] | 王艳海,段宝生,巴特尔,等. 鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌基因分型[J]. 检验医学,2014,29(2): 110-114. |
[3] | 倪语星. 关注分子技术在临床微生物检验中的应用[J]. 检验医学,2014,29(6): 581-583. |
[4] | 姚冬婷,应春妹,郑冰. 微卫星多态性和多位点序列分析技术在光滑念珠菌基因分型中的应用评估[J]. 检验医学,2014,29(6): 593-596. |
[5] | TARTOF SY,SOLBERG OD,MANGES AR,et al.Analysis of a uropathogenic Escherichia coli clonal group by multilocus sequence typing[J].J Clin Microbiol,2005,43(12):5860-5864. |
[6] | 潘伟光,李一凡,钟海萍,等.产超广谱β内酰胺酶大肠埃希菌多位点序列分型研究[J].中国感染与化疗杂志,2013,13(4):302-306. |
[7] | ZHANG L, LÜ X, ZONG Z.The emergence of blaCTX-M-15-carrying Escherichia coli of ST131 and new sequence types in Western China[J]. Ann Clin Microbiol Antimicrob,2013,12:35. |
[8] | BLANCO M, ALONSO MP, NICOLAS-CHANOINE MH, et al.Molecular epidemiology of Escherichia coli producing extended-spectrum beta-lactamases in Lugo (Spain): dissemination of clone O25b:H4-ST131 producing CTX-M-15[J]. J Antimicrob Chemother,2009,63(6):1135-1141. |
[9] | BLANCO J, MORA A, MAMANI R, et al.Four main virotypes among extended-spectrum-β-lactamase-producing isolates of Escherichia coli O25b:H4-B2-ST131: bacterial,epidemiological, and clinical characteristics[J]. J Clin Microbiol,2013, 51(10):3358-3367. |
[10] | NICOLAS-CHANOINE MH, BLANCO J, LEFLON-GUIBOUT V, et al.Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15[J]. J Antimicrob Chemother,2008,61(2):273-281. |
[11] | ROGERS BA, SIDJABAT HE, PATERSON DL.Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain[J]. J Antimicrob Chemother, 2011,66(1):1-14. |
[12] | RUIZ SJ, MONTEALEGRE MC, RUIZ-GARBAJOSA P, et al.First characterization of CTX-M-15-producing Escherichia coli ST131 and ST405 clones causing community-onset infections in south America[J]. J Clin Microbiol,2011,49(5):1993-1996. |
[13] | NASEER U, HALDORSEN B, TOFTELAND S, et al.Molecular characterization of CTX-M-15-producing clinical isolates of Escherichia coli reveals the spread of multidrug-resistant ST131 (O25:H4) and ST964 (O102:H6) strains in Norway[J]. APMIS,2009, 117(7):526-536. |
[14] | TIAN GB, RIVERA JI, PARK YS, et al.Sequence type ST405 Escherichia coli isolate producing QepA1, CTX-M-15,and RmtB from Detroit, Michigan[J]. Antimicrob Agents Chemother,2011,55(8):3966-3967. |
[15] | NOVAIS Â,VUOTTO C, PIRES J, et al.Diversity and biofilm-production ability among isolates of Escherichia coli phylogroup D belonging to ST69, ST393 and ST405 clonal groups[J]. BMC Microbiol,2013,13:144. |
[16] | SIDJABAT HE, PATERSON DL, ADAMS-HADUCH JM, et al.Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western Pennsylvania[J]. Antimicrob Agents Chemother,2009,53(11):4733-4739. |
[17] | CORTÉS P, BLANC V, MORA A, et al.Isolation and characterization of potentially pathogenic antimicrobial-resistant Escherichia coli strains from chicken and pig farms in Spain[J]. Appl Environ Microbiol,2010,76(9):2799-2805. |
[18] | EWERS C, BETHE A, STAMM I, et al.CTX-M-15-D-ST648 Escherichia coli from companion animals and horses: another pandemic clone combining multiresistance and extraintestinal virulence[J]. J Antimicrob Chemother,2014,69(5):1224-1230. |
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