Abstract: Objective To investigate the application significance of coagulation factor Ⅴ and Ⅷ activities (FⅤ:C and Ⅷ:C) and tissue factor(TF) in diagnosis of type 2 diabetes mellitus (T2DM) combined with coronary heart disease(CHD). Methods FⅤ:C, FⅧ:C, TF, von Willibrand factor(VWF) activity and platelet aggregation rate(PAR) were measured in 85 patients with T2DM combined with CHD [T2DM with CHD group, including T2DM with acute myocardial infarction (AMI) group (19 cases), T2DM with old myocardial infarction (OMI) group (23 cases), T2DM with unstable angina pectoris (UAP) group (25 cases) and T2DM with stable angina pectoris(SAP) group (18 cases)], 29 T2DM patients without complication (single T2DM group) and 24 healthy controls (control group). The correlation between FⅤ:C, FⅧ:C and TF with VWF and PAR was analyzed. Results The levels of FⅤ:C, FⅧ:C and TF in T2DM with CHD group and single T2DM group were remarkably higher than those in control group (P<0.001), and those in T2DM with CHD group were higher than those in single T2DM group(P<0.01). In T2DM with AMI group and T2DM with UAP group, the levels of FⅤ:C, FⅧ:C and TF were markedly higher than those in single T2DM group(P<0.001), and those in T2DM with AMI group were higher than those in T2DM with UAP group(P<0.01). In T2DM with OMI group and T2DM with SAP group, the TF levels were significantly higher than those in single T2DM group(P<0.01), but there was no statistical significance among the 3 groups for FⅤ:C and FⅧ:C levels(P>0.05).There was a significantly positive correlation of FⅤ:C, FⅧ:C and TF levels with VWF and PAR levels(P<0.01). Results FⅤ:C, FⅧ:C and TF can be as the indicators for T2DM with CHD. TF is useful for the evaluation of disease development in patients with T2DM with CHD.

Key words: Coagulation factor Ⅴ, Coagulation factor Ⅷ, Tissue factor, Type 2 diabetes mellitus, Coronary heart disease