慢性乙型肝炎患者外周血抗原特异性CD8+T细胞功能的初步分析

doi:10.3969/j.issn.1673-8640.2014.01.003

检验医学 ›› 2014, Vol. 29 ›› Issue (1): 10-14.DOI: 10.3969/j.issn.1673-8640.2014.01.003

• 乙型肝炎病毒感染者细胞免疫的临床监测专题 • 上一篇下一篇

慢性乙型肝炎患者外周血抗原特异性CD8⁺T细胞功能的初步分析

蔡刚¹, 吴蓓颖¹, 范臻佳¹, 沈茜²

1.上海交通大学医学院附属瑞金医院检验科, 上海 200025;
2.第二军医大学附属长海医院实验诊断科, 上海 200433

收稿日期:2013-07-27 出版日期:2014-01-20 发布日期:2014-01-20
通讯作者: 沈茜, 联系电话:021-31162063。
作者简介:蔡刚, 男, 1975年生, 博士, 副主任技师, 主要从事分子生物学和流式细胞术检测工作。

Primary analysis on the function of peripheral blood HBV-specific CD8⁺T cells from patients with chronic hepatitis B

CAI Gang¹, WU Beiying¹, FAN Zhenjia¹, SHEN Qian².

1.Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China; 2. Department of Laboratory Diagnosis, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China

Received:2013-07-27 Online:2014-01-20 Published:2014-01-20

摘要/Abstract

摘要：

目的评价慢性乙型肝炎(CHB)患者外周血抗原特异性CD8⁺T细胞的功能及与临床相关指标的关系。方法采用主要组织相容性复合物(MHC)-抗原肽四聚体标记技术检测CHB患者外周血乙型肝炎病毒(HBV)抗原特异性CD8⁺T细胞, 并对其细胞因子分泌能力进行检测, 分析其与临床生化和病毒学指标的关系。结果 35例人类白细胞相关抗原(HLA)-A基因型为A02或A24的患者中有9例患者至少检出1种被四聚体识别的HBV抗原特异性CD8⁺ T细胞, 其白细胞介素2(IL-2)和γ干扰素(IFN-γ)的分泌能力明显低于其自身的外周血总CD8⁺ T细胞。检出抗原特异性CD8⁺T细胞患者的血清丙氨酸氨基转移酶(ALT)水平明显高于阴性患者, 而HBV DNA水平低于阴性患者。结果 CHB患者外周血抗原特异性CD8⁺T细胞存在明显的功能缺陷, 可能因病毒抗原持续刺激造成功能耗竭, 但外周血出现抗原特异性CD8⁺T细胞仍表明患者体内出现较强抗病毒免疫反应。

关键词: T细胞耗竭, 慢性乙型肝炎, 四聚体技术

Abstract:

Objective To evaluate the function of peripheral blood hepatitis B virus(HBV)-specific CD8⁺ T cells from patients with chronic hepatitis B(CHB), and assess its relationship with clinical relative parameters. Methods The HBV-specific CD8⁺ T cells were detected by staining of major histocompatibility complex (MHC)-peptide tetramers in patients with CHB. The HBV-specific CD8⁺ T cells were analyzed for cytokine secretion ability. The relationship of HBV-specific CD8⁺ T cells with clinical biochemical and viral indices was analyzed. Results A total of 9 of 35 patients with human leukocyte antigen (HLA)-A02 genotype or A24 genotype had HBV-specific CD8⁺ T cells recognized by tetramers in peripheral blood. The HBV-specific CD8⁺ T cells expressed significantly lower interleukin 2(IL-2) and interferon gamma(IFN-γ) than their counterpart CD8⁺ T cells. The patients who can be found HBV-specific CD8⁺ T cells had higher level of alanine aminotransferase(ALT) and lower HBV DNA load than those without HBV-specific CD8⁺ T cells. Results Persistent stimulation of HBV antigen is one of the causes of HBV-specific CD8⁺ T cell dysfunction in patients with CHB. The patients who can be found HBV-specific CD8⁺ T cells in peripheral blood could have strong anti-virus immunity.

Key words: T cell exhaustion, Chronic hepatitis B, Major histocompatibility complex-peptide tetramer

中图分类号:

R446.63

蔡刚, 吴蓓颖, 范臻佳, 沈茜. 慢性乙型肝炎患者外周血抗原特异性CD8⁺T细胞功能的初步分析[J]. 检验医学, 2014, 29(1): 10-14.

CAI Gang, WU Beiying, FAN Zhenjia, SHEN Qian.. Primary analysis on the function of peripheral blood HBV-specific CD8⁺T cells from patients with chronic hepatitis B[J]. , 2014, 29(1): 10-14.

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慢性乙型肝炎患者外周血抗原特异性CD8⁺T细胞功能的初步分析

Primary analysis on the function of peripheral blood HBV-specific CD8⁺T cells from patients with chronic hepatitis B

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慢性乙型肝炎患者外周血抗原特异性CD8⁺T细胞功能的初步分析

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