酶转换法检测同型半胱氨酸的评价及其对冠心病的早期诊断价值

检验医学 ›› 2012, Vol. 27 ›› Issue (7): 571-574.

酶转换法检测同型半胱氨酸的评价及其对冠心病的早期诊断价值

邢晓光

天津港口医院，天津 300456

收稿日期:2012-05-24 修回日期:2012-03-08 出版日期:2012-07-30 发布日期:2012-07-05
作者简介:邢晓光，男，1971年生，学士，副主任技师，主要从事免疫及临床检验工作。

Evaluation on the homocysteine detection by enzyme transition method and the early diagnostic significance of homocysteine to coronary heart disease

Tianjin Port Hospital，Tianjin 300456，China

Received:2012-05-24 Revised:2012-03-08 Online:2012-07-30 Published:2012-07-05

摘要/Abstract

摘要： 目的　探讨酶转换法检测同型半胱氨酸（Hcy）的意义及其对冠心病的诊断价值。方法　对酶转换法检测Hcy的精密度、线性试验、干扰试验、回收试验、灵敏度进行方法学评价，并与高效液相色谱法（HPLC）进行对比。采用酶转换法检测60例冠心病患者、54例血脂和血糖水平正常但有动脉粥样硬化疾病家族史的患者（患者对照组）及55名健康对照者的血浆Hcy，同时检测血糖（Glu）、总胆固醇（TC）及甘油三酯（TG）水平。结果　酶转换法检测Hcy的批内不精密度（CV）分别为1.98%、2.34%；批间CV分别为2.13%、1.87%；日间CV分别为2.47%、1.33%；总CV分别为3.85%、2.41%。在2～80 μmol/L范围内有良好的线性关系（r²＝0.987 4）；TG＜20 mmol/L、总胆红素（TBil）＜300 μmol/L、血红蛋白（Hb）＜10 g/L对酶转换法检测Hcy无明显干扰（P均＞0.05）；回收率为98.9%～102.4%；分析灵敏度为0.27 μmol/L。对2种方法进行直线回归分析，酶转换法与HPLC相关性较好（r＝0.935 2），2种方法的结果差异无统计学意义（P＞0.05）。Hcy与Glu、TG呈正相关（r分别为0.447、0.416，P＜0.01），与TC无相关性（r＝0.146，P＞0.05）。冠心病组、患者对照组血浆Hcy水平均明显高于正常对照组（P均＜0.01）。冠心病组TG、Glu水平高于患者对照组及正常对照组（P均＜0.01）。结论　酶转换法具有精密度良好、灵敏度较高、线性范围较宽、抗干扰能力强、结果准确等特点，适合基层临床实验室推广。血浆Hcy水平升高对于冠心病具有重要价值。

关键词: 同型半胱氨酸, 冠心病, 酶转换法, 方法学评价

Abstract: Objective　To investigate the significance of detecting homocysteine（Hcy） by enzyme transition method and the early diagnostic significance of Hcy to coronary heart disease. Methods　The enzyme transition method for detecting Hcy was evaluated by methodology for precision，linearity，interference，recovery and sensitivity tests，and was compared to high performance liquid chromatography（HPLC） method. The levels of Hcy among 60 coronary heart disease patients，55 healthy controls and 54 patient controls with normal blood lipid and glucose（Glu） levels and atherosclerosis family medical history were detected by enzyme transition method，and the levels of Glu，total cholesterol（TC） and triglyceride（TG） were detected. Results　The within-run imprecisions（CV） were 1.98% and 2.34% respectively. The between-run CV were 2.13% and 1.87% respectively. The interday CV were 2.47% and 1.33% respectively. The total CV were 3.85% and 2.41% respectively. There was a good linear relationship between 2-80 μmol/L（r²＝0.987 4）. Almost no significant interference can be observed in the enzyme transition method to detect Hcy of TG＜20 mmol/L，total bilirubin（TBil）＜300 μmol/L and hemoglobin（Hb）＜10 g/L（P＞0.05）. The recovery rate was 98.9%-102.4%. The sensitivity was 0.27μmol/L. There was a good correlation between the enzyme transition method and HPLC method by linear-correlated analysis（r＝0.935 2），and there was no significant difference between the 2 methods（P＞0.05）. The positive correlation of Hcy with Glu and TG（r＝0.447 and 0.426，P＜0.01），and no correlation between Hcy and TC were shown（r＝0.146，P＞0.05）. The levels of Hcy in coronary heart disease group and patient control group were higher than those in healthy control group（P＜0.01）. The levels of TG and Glu in coronary heart disease group were higher than those in patient control group and healthy control group（P＜0.01）. Conclusions　Enzyme transition method is suitable for clinical laboratory with the advantages of good precision，high sensitivity，wide linear range，good anti-interference ability and accurate results. It has a significance for the early diagnosis of coronary heart disease when the level of Hcy rises.

Key words: Homocysteine, Coronary heart disease, Enzyme transition method, Methodology evaluation

中图分类号:

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邢晓光. 酶转换法检测同型半胱氨酸的评价及其对冠心病的早期诊断价值[J]. 检验医学, 2012, 27(7): 571-574.

XING Xiaoguang. Evaluation on the homocysteine detection by enzyme transition method and the early diagnostic significance of homocysteine to coronary heart disease[J]. , 2012, 27(7): 571-574.

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