子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义

doi:10.3969/j.issn.1673-8640.2015.05.011

检验医学 ›› 2015, Vol. 30 ›› Issue (5): 454-456.DOI: 10.3969/j.issn.1673-8640.2015.05.011

子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义

刘洪正¹, 王玉亮²

1. 天津市滨海新区汉沽中医医院检验科,天津 300480
2. 天津市第一中心医院检验科卫生部危重病急救医学重点实验室,天津 300192

收稿日期:2014-12-12 出版日期:2015-05-30 发布日期:2015-06-17
作者简介:null
作者简介:刘洪正,男,1966年生,主管技师,主要从事临床免疫检验工作。

通讯作者:王玉亮,联系电话:022-23728992。

Expression of Fas in peritoneal fluid mononuclear cells among endometriosis patients and its significance

LIU Hongzheng¹, WANG Yuliang²

1. Department of Clinical Laboratory, Hangu Chinese Medicine Hospital of Tianjin Binhai New Area, Tianjin 300480, China
2. Department of Clinical Laboratory, Tianjin First Central Hospital, Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin 300192, China

Received:2014-12-12 Online:2015-05-30 Published:2015-06-17

摘要/Abstract

摘要： 目的探讨子宫内膜异位症与Fas介导的腹腔液单个核细胞凋亡的关系。方法选取手术后病理证实为子宫内膜异位症的患者55例作为子宫内膜异位症组,以非子宫内膜异位症患者52例作为对照组,分离腹腔液单个核细胞,应用实时逆转录聚合酶链反应(RT-PCR)测定Fas mRNA的表达水平,应用流式细胞术定量检测Fas表达细胞百分率。结果子宫内膜异位症组腹腔液单个核细胞Fas mRNA及Fas表达细胞百分率明显高于对照组(P<0.05),Fas mRNA及Fas表达细胞百分率在子宫内膜异位症组Ⅰ~Ⅱ期与Ⅲ~Ⅳ期中表达差异无统计学意义(P>0.05)。结论子宫内膜异位症患者腹腔液单个核细胞Fas表达异常,对子宫内膜异位症具有一定的作用。

关键词: Fas, 单个核细胞, 腹腔液, 子宫内膜异位症

Abstract: Objective To investigate the relationship of Fas expression in peritoneal fluid mononuclear cells and endometriosis. Methods A total of 55 patients with histopathologically diagnosed endometriosis after operation were enrolled as endometriosis group, and 52 patients without endometriosis were enrolled as control group. The peritoneal fluid mononuclear cells were isolated, the expression levels of Fas mRNA were determined by reverse transcriptase-polymerase chain reaction(RT-PCR), and the percentage of Fas expression cells were quantitatively detected by flow cytometry. Results Fas mRNA and the percentage of Fas expression cells were significantly higher in endometriosis group than those in control group(P<0.05). There was no statistical significance between stage Ⅰ-Ⅱand stage Ⅲ-Ⅳ(P>0.05). Conclusions The abnormal Fas expression in peritoneal fluid mononuclear cells may play an important role in endometriosis.

Key words: Fas, Mononuclear cell, Peritoneal fluid, Endometriosis

中图分类号:

R446.1

刘洪正, 王玉亮. 子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义[J]. 检验医学, 2015, 30(5): 454-456.

LIU Hongzheng, WANG Yuliang. Expression of Fas in peritoneal fluid mononuclear cells among endometriosis patients and its significance[J]. Laboratory Medicine, 2015, 30(5): 454-456.

图/表 1

参考文献 12

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组别	例数	Fas mRNA	Fas表达细胞(%)
子宫内膜异位症组	55	1.5±0.7^*	19.10±3.90^*
Ⅰ~Ⅱ期	38	1.2±0.6	17.83±1.45
Ⅲ~Ⅳ期	17	1.4±0.7	20.04±4.81
对照组	52	1.1±0.4	16.38±1.17

组别	例数	Fas mRNA	Fas表达细胞(%)
子宫内膜异位症组	55	1.5±0.7^*	19.10±3.90^*
Ⅰ~Ⅱ期	38	1.2±0.6	17.83±1.45
Ⅲ~Ⅳ期	17	1.4±0.7	20.04±4.81
对照组	52	1.1±0.4	16.38±1.17

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子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义

Expression of Fas in peritoneal fluid mononuclear cells among endometriosis patients and its significance

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