慢性阻塞性肺疾病患者外周血T淋巴细胞凋亡检测的临床价值

doi:10.3969/j.issn.1673-8640.2015.10.001

摘要/Abstract

摘要： 目的

探讨慢性阻塞性肺疾病(COPD)患者外周血CD4⁺、CD8⁺ T细胞凋亡水平与肺功能的相关性。

方法

采用流式细胞术测定30例COPD缓解期患者、30例COPD急性期患者及20名健康体检者外周血CD4⁺和CD8⁺ T细胞凋亡水平,与第1秒用力呼出量占预计值百分比(FEV1%)和FEV1/用力肺活量(FVC)水平作相关分析。

结果

COPD患者CD4⁺ T细胞凋亡水平较健康对照组明显升高(P<0.01),其中急性期显著高于缓解期(P<0.05);COPD患者CD8⁺T细胞凋亡水平明显低于健康对照组(P<0.01),其中急性期明显低于缓解期(P<0.05)。CD4⁺ T细胞凋亡率与FEV1%、FEV1/FVC呈负相关(P<0.01);CD8⁺ T细胞凋亡率与FEV1%、FEV1/FVC呈正相关(P<0.01)。

结论

CD4⁺ 、CD8⁺ T细胞凋亡水平与肺功能具有密切相关性,其水平变化可作为监测COPD患者病情的重要指标。

关键词: 细胞凋亡, T淋巴细胞亚群, 肺功能, 慢性阻塞性肺疾病

Abstract: Objective

To investigate the correlation of the apoptosis of peripheral blood CD4⁺ and CD8⁺ T cells with lung function in chronic obstructive pulmonary disease (COPD)patients.

Methods

A total of 30 stable COPD patients, 30 acute exacerbation COPD patients and 20 healthy subjects were enrolled. The apoptosis of peripheral blood CD4⁺ and CD8⁺ T cells was determined by flow cytometry. The correlation of T lymphocyte apoptosis with forced expiratory volume 1 second (FEV1%) and FEV1/forced vital capacity (FVC) was analyzed.

Results

The CD4⁺ T cell apoptosis of COPD group increased significantly with that of healthy control group(P<0.01), and that of acute exacerbation group was higher than that of stable group (P<0.05). The CD8⁺ T cell apoptosis of COPD group was lower than that of healthy control group (P<0.01) . The CD8⁺ T cell apoptosis of acute exacerbation group was lower than that of stable group(P<0.05). CD4⁺ T cell apoptosis was negatively correlated with FEV1% and FEV1/FVC, and CD8⁺ T cell apoptosis was positively correlated with FEV1% and FEV1/FVC (P<0.01).

Conclusions

The apoptosis of CD4⁺ and CD8⁺ T cells is closely related to lung function in COPD patients, which can be used as an indicator for the monitor of COPD.

Key words: Cell apoptosis, T lymphocyte subset, Lung function, Chronic obstructive pulmonary disease

中图分类号:

R446.63

廖长征, 徐文莉, 陈思祖. 慢性阻塞性肺疾病患者外周血T淋巴细胞凋亡检测的临床价值[J]. 检验医学, 2015, 30(10): 971-974.

LIAO Changzheng, XU Wenli, CHEN Sizu.. Clinical significance of peripheral blood T lymphocyte apoptosis determination for chronic obstructive pulmonary disease patients[J]. Laboratory Medicine, 2015, 30(10): 971-974.

图/表 4

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组别	例数	CD4⁺ T细胞凋亡率	CD8⁺ T细胞凋亡率	FEV1%	FEV1/FVC
健康对照组	20	15.14±2.17	29.29±4.58	92.2±9.8	90.2±8.7
COPD缓解期组1	30	25.19±3.73^*	25.13±3.17^*	53.1±10.5^*	56.5±10.1^*
COPD急性期组	30	29.98±4.15^*#	20.47±5.18^*#	45.3±9.5^*#	47.5±11.2^*#
COPD缓解期组2	20	24.89±5.87^*△	25.75±5.15^*△	52.6±9.5^*△	55.9±10.8^*△

组别	例数	CD4⁺ T细胞凋亡率	CD8⁺ T细胞凋亡率	FEV1%	FEV1/FVC
健康对照组	20	15.14±2.17	29.29±4.58	92.2±9.8	90.2±8.7
COPD缓解期组1	30	25.19±3.73^*	25.13±3.17^*	53.1±10.5^*	56.5±10.1^*
COPD急性期组	30	29.98±4.15^*#	20.47±5.18^*#	45.3±9.5^*#	47.5±11.2^*#
COPD缓解期组2	20	24.89±5.87^*△	25.75±5.15^*△	52.6±9.5^*△	55.9±10.8^*△

项目	FEV1%		FEV1/FVC
项目	r值	P值	r值	P值
CD4⁺ T细胞凋亡率	-0.861	<0.01	-0.857	<0.01
CD8⁺ T细胞凋亡率	0.743	<0.01	0.771	<0.01

项目	FEV1%		FEV1/FVC
项目	r值	P值	r值	P值
CD4⁺ T细胞凋亡率	-0.861	<0.01	-0.857	<0.01
CD8⁺ T细胞凋亡率	0.743	<0.01	0.771	<0.01