关键词: 乙型肝炎病毒, 耐药, 突变, 基因型, 汉族, 维吾尔族

Abstract:

Objective To investigate the relationship between hepatitis B virus (HBV) mutational pattern and genotypes among chronic hepatitis B patients in Xinjiang. Methods Genotyping of 245 HBV DNA positive samples was performed by polymerase chain reaction (PCR). Lamivudine and adefovir associated resistant sites (180, 204, 181 and 236 sites) were analyzed by gene chips. Resutls There were 40 cases of HBV genotype B, 137 cases of HBV genotype C, 48 cases of HBV genotype C/D and 20 cases of other HBV genotype among the 245 chronic hepatitis B patients. The distribution was not significant statistically in different sex and ages (χ²=1.791, P=0.617; χ²=14.214, P=0.115), but there was statistical significance in different ethnicities(χ²=83.076, P=0.000). The mutational detection rates for HBV at 180, 204, 181 and 236 sites were 33.8%, 76.8%, 66.9% and 45.0%, with statistical significance (P<0.05). The distribution of HBV genotypes among the common mutational pattern had no statistical difference(χ²=14.969, P=0.454), but HBV genotype C showed the highest proportion among the common mutational pattern. Conclusions HBV genotypes have different distribution in different ethnicities. The mutational information in common resistant sites is different. The distribution of HBV genotypes is not different in common mutational pattern.

Key words: Hepatitis B virus, Drug resistance, Mutation, Genotype, Han population, Uighur population