血清葡萄糖氧化酶法新色原物的实验研究

检验医学 ›› 2012, Vol. 27 ›› Issue (10): 799-802.

血清葡萄糖氧化酶法新色原物的实验研究

王宝占,李立和,魏志斌,刘冰

1.天津医科大学宝坻临床学院检验科，天津 301800；2.天津医科大学检验学院，天津 300203

收稿日期:2012-07-13 修回日期:2012-07-24 出版日期:2012-10-30 发布日期:2012-10-12
作者简介:王宝占，男，1973年生，学士，主管技师，主要从事临床生物化学方法学研究。

Study on a new chromogenic substrate in detection of glucose in serum by glucose oxidase

1. Department of Clinical Laboratory，Baodi Clinical Institute，Tianjin Medical University，Tianjin 301800，China；2. Institute of Laboratory Medicine, Tianjin Medical University, Tianjin 300203, China

Received:2012-07-13 Revised:2012-07-24 Online:2012-10-30 Published:2012-10-12

摘要/Abstract

摘要： 目的　以N-(2-羟基-3-磺丙基)-3，5-二甲氧基苯胺钠盐（HDAOS）为新色原物，建立新的葡萄糖氧化酶（GOD）法检测血清葡萄糖。方法　用HDAOS代替4-氯酚生成蓝色醌亚胺，测定波长为600 nm。采用本法、葡萄糖氧化酶-过氧化物酶-4-氨基安替比林-酚（GOD-PAP）法及高效液相色谱（HPLC）法同时测定脂血、溶血、黄疸及外观正常的血清各24份并比较结果，同时对本法进行方法学评价。结果　在测定外观正常血清时，3种方法之间差异均无统计学意义(P＞0.05)；在测定脂血、溶血、黄疸血清时，本法葡萄糖测定结果与GOD-PAP法比较，差异有统计学意义(P＜0.05)；与HPLC法比较，差异无统计学意义(P＞0.05)。本法批内、批间变异系数（CV）均＜3.0%；与HPLC法呈良好相关性（r²=0.995 8）；在5 min内均可达到反应终点；线性范围为0.20～28.00 mmol/L；血红蛋白＜2.0 g/L、乳糜＜2.2%、总胆红素＜200 μmol/L对本法的干扰误差＜3%；参考范围为4.10～6.20 mmol/L（男）、4.04～6.15 mmol/L（女）；最大吸收波长为595 nm。结论　以HDAOS代替4-氯酚生成蓝色醌亚胺，能通过降低脂血、溶血、黄疸光谱吸收的方法消除脂血、溶血和黄疸的干扰，提高GOD法测定葡萄糖的准确性，其使用方法与原有GOD终点法相同。

关键词: 葡萄糖, 葡萄糖氧化酶法, N-(2-羟基-3-磺丙基)-3, 5-二甲氧基苯胺钠盐

Abstract: Objective　To establish the glucose oxidase (GOD) method using a new chromogenic substrate N-(2-hydroxy-3-sulfopropyl)-3，5-dimethoxyaniline sodium salt (HDAOS) monohydrate in the detection of glucose in serum. Methods　　The tetrachlorophenol was replaced by HDAOS, the blue quinone-imine was formed, and it was measured at 600 nm. The GOD method, glucose oxidase-peroxidase-4-aminoantipyrene-phenol (GOD-PAP) and high performance liquid chromatography (HPLC) were used to determine serum hyper lipidemia samples (24 samples), haemolysis samples (24 samples), choloplania samples (24 samples) and normal samples (24 samples), and the results were compared. The methodology evaluation was performed. Results　There was no statistical significance among the 3 methods, when the normal serum group was measured (P＞0.05). Statistical significance only existed between the GOD method and GOD-PAP, when the serum groups of hyperlipidemia，hemolysis and choloplania were measured (P＜0.05), and there was no statistical significance between the GOD method and HPLC (P＞0.05). The between-run and within-run coefficients of variation (CV) were<0.3%. The GOD method had a good correlation with HPLC (r²=0.995 8), and got to the reaction end point within 5 min. The linear range was 0.20-28.00 mmol/L. When hemoglobin <2.0 g/L, chyle<2.2% and total bilirubin <200 μmol/L, the interference error for the GOD method was <3%. The reference values were 4.10-6.20 mmol/L for males and 4.04-6.15 mmol/L for females，and the maximal absorbing wavelength was 595 nm. Conclusions　Replacing tetrachlorophenol with HDAOS, the blue quinone-imine is formed. The GOD method eliminates the interference of hyperlipidemia，haemolysis and choloplania through limiting their absorption spectra. The GOD method has a good measurement accuracy in the detection of glucose and performs the same reaction end point.

Key words: Glucose, Glucose oxidase method, N-(2-hydroxy-3-sulfopropyl)-3, 5-dimethoxyaniline sodium salt

王宝占1，李立和1，魏志斌2，刘冰2. 血清葡萄糖氧化酶法新色原物的实验研究[J]. 检验医学, 2012, 27(10): 799-802.

WANG Baozhan 1，LI Lihe 1，WEI Zhibin 2，LIU Bing 2. Study on a new chromogenic substrate in detection of glucose in serum by glucose oxidase[J]. , 2012, 27(10): 799-802.

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