检验医学 ›› 2022, Vol. 37 ›› Issue (7): 652-656.DOI: 10.3969/j.issn.1673-8640.2022.07.011

• 技术研究与评价·论著 • 上一篇    下一篇

UroQuick尿液快速培养技术在儿童尿路感染诊断中的应用

潘芬, 潘雨萱, 石迎迎, 于方圆, 张泓()   

  1. 上海市儿童医院 上海交通大学医学院附属儿童医院检验科,上海 200062
  • 收稿日期:2020-11-22 修回日期:2021-10-14 出版日期:2022-07-30 发布日期:2022-08-26
  • 通讯作者: 张泓
  • 作者简介:张 泓,E-mail: zhanghong3010@vip.126.com
    潘 芬,女,1988年生,硕士,主管技师,主要从事临床微生物学检测工作。
  • 基金资助:
    上海市重点临床专科建设项目(shslczdzk06902)

UroQuick rapid urine culture technique for urinary tract infection in children

PAN Fen, PAN Yuxuan, SHI Yingying, YU Fangyuan, ZHANG Hong()   

  1. Department of Clinical Laboratory,Shanghai Children's Hospital, Children's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200062,China
  • Received:2020-11-22 Revised:2021-10-14 Online:2022-07-30 Published:2022-08-26
  • Contact: ZHANG Hong

摘要:

目的 探讨UroQuick尿液快速培养技术在儿童尿路感染(UTI)诊断中的应用价值。方法 收集2019年12月—2020年1月上海市儿童医院0~14岁疑似UTI患儿清洁中段尿样本235例,同时进行常规定量细菌培养和UroQuick快速培养。以常规培养结果为金标准,评价UroQuick快速培养技术对UTI的诊断效能。结果 235例清洁中段尿样本中,常规培养阳性58例(24.7%),有43例检出细菌、4例检出真菌、11例2种病原均检出;UroQuick快速培养阳性69例(29.4%),有55例检出细菌、3例检出真菌、11例2种病原均检出。2种方法一致率为86.8%(204/235)。以常规培养结果为金标准,以UroQuick检测的浊度结果作为计算原则,UroQuick快速培养诊断UTI的敏感性为84.48%,特异性为88.70%,阳性预测值为71.01%,阴性预测值为94.58%;以UroQuick检测的菌量浓度结果作为计算原则,UroQuick快速培养诊断UTI的敏感性为82.76%,特异性为92.66%,阳性预测值为78.69%,阴性预测值为94.25%。结论 UroQuick快速培养作为一种新的尿液快速培养技术,无论按照浊度结果还是按菌量浓度结果计算,均具有较好的特异性和阴性预测值,可为儿童UTI的早期筛查提供一定的帮助。

关键词: 尿路感染, 尿液培养, UroQuick快速培养法, 儿童

Abstract:

Objective To investigate the role of UroQuick rapid urine culture technique for urinary tract infection(UTI) in children. Methods A total of 235 clean midstream urine samples were collected from children with suspected UTI aged 0-14 years from December 2019 to January 2020. Conventional urine culture and UroQuick rapid urine culture were performed simultaneously. Taking the results of conventional urine culture as gold standard,the diagnostic efficacy of UroQuick rapid urine culture for UTI was further evaluated. Results Among the 235 samples,58(24.7%) samples,including 43 samples of bacteria,4 samples of fungi and 11 samples of bacteria and fungi,were positive by conventional urine culture,while 69 samples,including 55 samples of bacteria,3 samples of fungi and 11 samples of bacteria and fungi,were positive by UroQuick rapid urine culture. The consistency rate of the 2 methods was 86.8%(204/235). Compared to conventional urine culture,the sensitivity,specificity,positive predictive value and negative predictive value of turbidity value of UroQuick rapid urine culture were 84.48%,88.70%,71.01% and 94.58%,respectively. Furthermore,the sensitivity,specificity,positive predictive value and negative predictive value of bacterial concentration of UroQuick rapid urine culture were 82.76%,92.66%,78.69% and 94.25%,respectively. Conclusions UroQuick rapid urine culture is a new technique for urine culture and has high specificity and negative predictive value whether in turbidity value or in bacterial concentration,which will provide a reference for the early screening of UTI in children.

Key words: Urinary tract infection, Urine culture, UroQuick rapid urine culture, Children

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