关键词: 亚甲基四氢叶酸还原酶基因, 多态性, 同型半胱氨酸, H型高血压

Abstract:

Objective To investigate the correlations of hypertension,serum homocysteine（Hcy） and methylenetetrahydrofolate reductase（MTHFR） C677T polymorphism with H type hypertension in Kunming Han population,and to provide basic information on the prevention and individualized treatment of H type hypertension. Methods A total of 111 patients with H type hypertension were enrolled,and 112 hypertension patients without H type hypertension were enrolled as non-H type hypertension group. Totally,119 healthy subjects were enrolled as healthy control group. Allele specific-polymerase chain reaction（AS-PCR） was used to determine MTHFR C677T polymorphism. Serum Hcy level was determined as well. The risk factors for H type hypertension were analyzed by Logistic regression analysis. Results MTHFR C677T CC,CT and TT genotype frequencies were 49.6%,36.1% and 14.3% in healthy control group. CC,CT and TT genotype frequencies were 24.3%,50.5% and 25.2% in H type hypertension group,and those were 45.5%,35.7% and 18.8% in non-H type hypertension group. The distribution frequencies of CT and TT genotypes in H type hypertension group were higher than those in non-H type hypertension group and healthy control group（P<0.05）. Logistic regression analysis showed that age,MTHFR C677T mutation,the family history of hypertension and drinking were risk factors for H type hypertension,the risk of H type hypertension caused by MTHFR C677T mutation was 2.701 times than those of control groups,and the risk of MTHFR C677T mutation was higher than those of other factors. Conclusions MTHFR C677T allele and polymorphism distribution frequency have regional characteristics among healthy subjects and hypertension patients in Kunming Han population. MTHFR C677T mutation may play an important role in the mechanism of H type hypertension for Kunming Han population.

Key words: Methylenetetrahydrofolate reductase, Polymorphism, Homocysteine, H type hypertension