检验医学 ›› 2017, Vol. 32 ›› Issue (5): 415-420.DOI: 10.3969/j.issn.1673-8640.2017.05.16

• 技术研究与评价·论著 • 上一篇    下一篇

3种鉴定系统在微生物室间质量评价中的应用和比较

陈蓉1, 居颺2, 刘战民1   

  1. 1.上海大学生命科学学院,上海 200444
    2.上海市普陀区长征镇社区卫生服务中心,上海 200333
  • 收稿日期:2016-11-24 出版日期:2017-05-20 发布日期:2017-06-06
  • 作者简介:null

    作者简介:陈 蓉,女,1979年生,学士,主管技师,主要从事微生物检测和质量管理工作。现在上海市临床检验中心工作。

    通信作者:刘战民,联系电话:021-66135166。

Application and comparison of 3 identification systems in microbiological external quality assessment

CHEN Rong1, JU Yang2, LIU Zhanmin1   

  1. 1. College of Life Sciences,Shanghai University,Shanghai 200444,China
    2. Putuo District Changzheng Town Community Health Service Center,Shanghai 200333,China
  • Received:2016-11-24 Online:2017-05-20 Published:2017-06-06

摘要:

目的 评价基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)、Vitek 2 Compact全自动微生物鉴定系统(简称Vitek 2 Compact)和MicroScan WalkAway 96 plus全自动微生物鉴定系统(简称MicroScan WalkAway 96 plus)在质量控制中的鉴定应用价值。方法 采用MALDI-TOF MS、Vitek 2 Compact和MicroScan WalkAway 96 plus同时对61株不同批次的质控菌株进行鉴定并作比较。结果 MALDI-TOF MS、Vitek 2 Compact和MicroScan WalkAway 96 plus对26株革兰阴性杆菌的鉴定准确率分别为88.4%、100.0%和84.6%,对15株革兰阳性球菌的鉴定准确率分别为100.0%、100.0%和86.7%,对8株酵母样真菌的鉴定准确率分别为100.0%、100.0%和87.5%,对12株其他细菌的鉴定准确率分别为100.0%、83.3%和41.7%。结论 MALDI-TOF MS、Vitek 2 Compact和MicroScan WalkAway 96 plus 3个鉴定系统对常见的革兰阳性球菌、革兰阴性杆菌、酵母样真菌的鉴定准确性较好。在苛养菌和革兰阳性杆菌方面, MALDI-TOF MS和Vitek 2 Compact的鉴定准确性较MicroScan WalkAway 96 plus好。

关键词: 基质辅助激光解析电离飞行时间质谱, Vitek 2 Compact全自动微生物鉴定系统, MicroScan WalkAway 96 plus全自动微生物鉴定系统, 质量控制, 室间质量评价

Abstract:

Objective To evaluate matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS),Vitek 2 Compact and MicroScan WalkAway 96 plus automatic microbial identification systems in microbiological external quality assessment. Methodse A total of 61 isolates of quality control strains with different runs were identified by MALDI-TOF MS,Vitek 2 Compact and MicroScan WalkAway 96 plus. The results of 3 identification systems were compared. Results The accuracy rates of MALDI-TOF MS,Vitek 2 Compact and MicroScan WalkAway 96 plus for identifying 26 isolates of Gram-negative bacilli were 88.4%,100.0% and 84.6%,the accuracy rates for identifying 15 isolates of Gram-positive cocci were 100.0%,100.0% and 86.7%,the accuracy rates for identifying 8 isolates of yeast-like fungi were 100.0%,100.0% and 87.5%,and the accuracy rates for identifying other 12 isolates were 100.0%,83.3% and 41.7%,respectively. Conclusions The accuracy rates of the 3 identification systems for Gram-positive cocci,Gram-negative bacilli and yeast-like fungi are good. For fastidious bacteria and Gram-positive bacilli,the accuracy rates of MALDI-TOF MS and Vitek 2 Compact are higher than those of MicroScan WalkAway 96 plus.

Key words: Matrix-assisted laser desorption/ionization time of flight mass spectrometry, Vitek 2 Compact automatic microbial identification system, MicroScan WalkAway 96 plus automatic microbial identification system, Quality control, External quality assessment

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