关键词: 丙型肝炎病毒, Sanger测序, 基因亚型, 系统进化树

Abstract:

Objective To study the distribution of hepatitis C virus（HCV） genotype in Wuhan,and to analyze the phylogenetic tree of rare HCV sub-genotypes. Methods Plasma samples were collected from 258 patients with hepatitis C in Wuhan. Sanger sequencing based on HCV-nonstructural protein 5B（NS5B） was used to genotyping. The results were compared with the genotypes by NCBI Genotyping tool. The HCV sub-genotypes were obtained. The phylogenetic tree of rare HCV sub-genotypes was analyzed by MEGA5.0 software. Results HCV sub-genotypes were successfully identified in all patients,and 6 HCV sub-genotypes,including type 1b（71.32%）,type 2a（20.16%）,type 6a（3.49%）,type 3b（2.71%）,type 3a（1.16%） and type 1a（1.16%）,were determined. The phylogenetic tree of rare HCV sub-genotypes was constructed successfully in this region. Conclusions The dominant HCV sub-genotypes are type 1b and type 2a,and type 6a,type 3a,type 3b and type 1a are rare in Wuhan. HCV sub-genotype 3a may be imported from Japan. Most of type 3b may be imported from Vietnam, and sample 379 appears large variation. Type 6a may be imported from Vietnam.

Key words: Hepatitis C virus, Sanger sequencing, Sub-genotype, Phylogenetic tree