关键词: 甘露聚糖, 侵袭性念珠菌病, 诊断

Abstract:

Objective To investigate the determination of mannan for the diagnosis of invasive candidiasis. Methods Mannan concentrations were determined in 53 patients with invasive candidiasis,30 patients with bacterial infection,28 patients with skin Candida infection and 20 healthy subjects （healthy control group） by enzyme-linked immunosorbent assay （ELISA）. Receiver operating characteristic （ROC） curve was used to evaluate diagnostic efficacy,and freeze-and-thawing experiment was used to determine stability. Results According to the positive threshold of the kits （≥1.0 μg/L）,the positive rate of mannan in invasive candidiasis group was higher than those in bacterial infection,skin Candida infection and healthy control groups （P<0.01）. ROC curve showed that the diagnostic sensitivity and specificity were 66.1% and 96.2% at positive threshold of ≥1.0 μg/L. The diagnostic sensitivity and specificity were 73.6% and 87.1% at positive threshold of ≥0.6 μg/L,and its area under the curve was 0.829 （95% confidence interval was 0.749-0.908）. After freeze-and-thawing for 1-4 times,mannan concentrations decreased by times with statistical significance （P<0.05）. Conclusions Mannan concentration may be a marker for the early diagnosis of invasive candidiasis.

Key words: Mannan, Invasive candidiasis, Diagnosis