口腔白念珠菌3种致病相关分泌型水解酶检测方法的建立及应用

doi:10.3969/j.issn.1673-8640.2016.05.003

摘要/Abstract

摘要：

目的了解口腔白念珠菌3种致病相关分泌型水解酶的体外表达水平。方法分别采用牛血清白蛋白平板、卵黄琼脂平板和三丁酸甘油酯平板检测88株临床分离的口腔白念珠菌分泌型天冬氨酸蛋白酶（SAP）、磷脂酶（PL）和脂肪酶（Lip）的活性。结果 88株白念珠菌中有70株（79.5%）高表达SAP,有64株（72.7%）高表达PL,仅有1株（1.2%）高表达Lip,有67株（76.1%）中等表达Lip。SAP和PL的表达呈正相关（r = 0.221,P < 0.05）,Lip与SAP、PL均无相关性（r值分别为0.085、0.109,P值分别为0.432、0.311）。结论大部分口腔白念珠菌高表达SAP和PL,中等表达Lip。分泌型水解酶是口腔白念珠菌最主要的毒力因子之一。

关键词: 白念珠菌, 分泌型天冬氨酸蛋白酶, 磷脂酶, 脂肪酶

Abstract:

Objective To investigate in vitro expressions of 3 pathogenic secreted hydrolytic enzymes of oral Candida albicans. Methods The activities of secreted aspartyl proteinase（SAP）,phospholipase（PL） and lipase（Lip） from 88 isolates of oral Candida albicans were determined by bovine serum albumin agar,egg yolk agar and tributyrate agar,respectively. Results In the 88 isolates,70（79.5%） isolates showed high activity of SAP ,and 64（72.7%）isolates showed high activity of PL,while only 1（1.2% ） isolate showed high activity of Lip,and 67（76.1%） isolates produced medium activity of Lip. Positive correlation was found between SAP and PL（r = 0.221,P<0.05）,and no correlation was found between Lip and SAP or between Lip and PL（ r = 0.085 and 0.109,P = 0.432 and 0.311）. Conclusions Most of the isolates have high expressions of SAP and PL and medium expression of Lip. Secreted hydrolytic enzyme is one of the main virulence factors of oral Candida albicans.

Key words: Candida albicans, Secreted aspartyl proteinase, Phospholipase, Lipase

中图分类号:

R399.4

孙康德, 张家胜, 陈旭, 虞中敏, 陈福祥. 口腔白念珠菌3种致病相关分泌型水解酶检测方法的建立及应用[J]. 检验医学, 2016, 31(5): 355-358.

SUN Kangde, ZHANG Jiasheng, CHEN Xu, YU Zhongmin, CHEN Fuxiang. Methods for determining 3 pathogenic secreted hydrolytic enzymes of oral Candida albicans and their application[J]. Laboratory Medicine, 2016, 31(5): 355-358.

图/表 4

参考文献 18

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标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.98	0.95	0.87	0.79	0.69	0.60	0.54	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	0.97	0.96	0.95	0.95	0.95

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.98	0.95	0.87	0.79	0.69	0.60	0.54	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	0.97	0.96	0.95	0.95	0.95

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.87	0.75	0.60	0.47	–	–	–	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.87	0.75	0.60	0.47	–	–	–	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.99	0.96	0.88	0.80	0.71	0.66	–	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00