关键词: 金黄色葡萄球菌, 皮肤软组织感染, 分子特征, 耐药性

Abstract:

Objective To study the molecular characteristics and drug resistance of Staphylococcus aureus isolated from patients with skin and soft tissue infections（SSTI） at Nantong University Haian Hospital in 2014. Methods A total of 26 isolates of Staphylococcus aureus were collected from patients with SSTI at Nantong University Haian Hospital,and the isolates were identified by phenotypic tests. Drug susceptibility test was performed by disk diffusion method. Panton-Valentine leukocidin（pvl） gene was determined by polymerase chain reaction（PCR）. Staphylococcal cassette chromosome mec（SCCmec） typing was performed by multi-PCR,and Staphylococcus protein A（spa） gene typing and multi-locus sequence typing（MLST） were performed by PCR and sequencing.Results A total of 5 isolates of methicillin-resistant Staphylococcus aureus（MRSA） were determined. Among them,3 isolates were ST630-t4549-SCCmec-Ⅴ,1 isolate was ST59-t437-SCCmec-Ⅰ,and 1 isolate was ST5-t311-SCCmec-Ⅰ. There was no resistant isolate to vancomycin,teicoplanin,sulfamethoxazole,rifampicin,linezolid and fusidic acid,and there was no pvl-positive isolate as well. Conclusions The main pathogen is methicillin-sensitive Staphylococcus aureus（MSSA）in patients with SSTI in Haian area of Jiangsu province. Community-acquired MRSA clone is found in SSTI patients.

Key words: Staphylococcus aureus, Skin and soft tissue infections, Molecular characteristics, Drug resistance