色谱法在花生四烯酸代谢产物检测中的应用

doi:10.3969/j.issn.1673-8640.2015.08.019

摘要/Abstract

摘要：

花生四烯酸(AA)代谢产物是AA经脂质过氧化产生的一系列物质,参与多种生理及病理生理过程,也是多种疾病的生物标志物,准确的定性、定量分析在研究相关疾病的发生、发展机制,诊断和预测中具有重要价值。在目前已经发现的近70种AA代谢产物中,均为小分子化合物,部分化合物分子结构相似,常规检测技术无法分辨。随着色谱、质谱技术的发展,为AA代谢产物的测定提供了较为可靠的技术手段。文章简要综述了相关进展。

关键词: 色谱法, 质谱法, 花生四烯酸

Abstract:

Arachidonic acid (AA) metabolite is a series of material produced by lipid peroxidation of (AA), and it is involved in many physiological and pathological processes. AA metabolites may be potential biomarkers for some diseases. Determining their quality and quantity accurately is significant in the occurrence, development, diagnosis and prediction of some diseases. Nearly 70 kinds of AA metabolites have been found, and they are all small molecule compounds. Conventional determination technology can't distinguish AA metabolites as their similar molecular structure. The development of chromatography and mass spectrometry provides a reliable technical method for the determination of AA metabolites. This paper gives a general review of the related advances.

Key words: Chromatography, Mass spectrometry, Arachidonic acid

中图分类号:

R446

姜连生, 综述, 谭龙益, 审校. 色谱法在花生四烯酸代谢产物检测中的应用[J]. 检验医学, 2015, 30(8): 852-856.

JIANG Liansheng, TAN Longyi. Application of chromatography in the detection of arachidonic acid metabolites[J]. Laboratory Medicine, 2015, 30(8): 852-856.

图/表 1

参考文献 30

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项目	TLC	GC-MS	LC-MS
优点	操作简单、经济快速	高敏感性、高特异性	高敏感性、高特异性、高通量
缺点	对AA代谢产物的区分能力有限, 仅适于少数物质的检测	预处理过程繁琐,某些热不稳定物质无法检测	仪器设备成本高、维护成本高
预处理	萃取后可提高检测敏感性,但非必须过程	热不稳定的代谢产物需形成甲酯或乙酯后再检测	固相萃取、液液萃取等方法
应用	用于内源性PG、TX等的生成测定	用于沸点较低或热稳定的类花生酸的检测	适于生物样本中大多数AA代谢产物的检测

项目	TLC	GC-MS	LC-MS
优点	操作简单、经济快速	高敏感性、高特异性	高敏感性、高特异性、高通量
缺点	对AA代谢产物的区分能力有限, 仅适于少数物质的检测	预处理过程繁琐,某些热不稳定物质无法检测	仪器设备成本高、维护成本高
预处理	萃取后可提高检测敏感性,但非必须过程	热不稳定的代谢产物需形成甲酯或乙酯后再检测	固相萃取、液液萃取等方法
应用	用于内源性PG、TX等的生成测定	用于沸点较低或热稳定的类花生酸的检测	适于生物样本中大多数AA代谢产物的检测