慢性肾小球肾炎患者外周血Th1/Th2及CD28+细胞的变化及其临床意义

doi:10.3969/j.issn.1673-8640.2015.02.009

检验医学 ›› 2015, Vol. 30 ›› Issue (2): 141-144.DOI: 10.3969/j.issn.1673-8640.2015.02.009

慢性肾小球肾炎患者外周血Th1/Th2及CD28⁺细胞的变化及其临床意义

周韵娇¹, 李鹏², 张丽红², 钱英俊², 牛建英²

1.复旦大学附属上海市第五人民医院中心实验室,上海 200240
2.复旦大学附属上海市第五人民医院肾内科,上海 200240

收稿日期:2014-02-25 出版日期:2015-02-28 发布日期:2015-02-12
作者简介:null
作者简介:周韵娇,女,1981年生,硕士,主管技师,主要从事细胞免疫研究工作。

通讯作者:牛建英,联系电话:021-64308151。
基金资助:
闵行区科委自然基金项目(2010MHZ027)

The variation and its clinical significance of Th1/Th2 and CD28⁺ cells from peripheral blood in patients with chronic glomerulonephritis

ZHOU Yunjiao¹, LI Peng², ZHANG Lihong², QIAN Yingjun², NIU Jianying²

1.Central Laboratory, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China
2. Department of Nephrology, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China

Received:2014-02-25 Online:2015-02-28 Published:2015-02-12

摘要/Abstract

摘要： 目的

探讨慢性肾小球肾炎(CGN)患者外周血Th1/Th2及CD28⁺细胞百分率的变化在疾病进程中可能的细胞免疫学机制。

方法

采用流式细胞术检测50例CGN患者及30名正常人(对照组)外周血Th1/Th2和CD28⁺细胞的百分率,用SPSS 16.0软件对试验结果进行统计分析。

结果

与对照组比较,CGN患者外周血CD3⁺CD4⁺细胞百分率和CD4/CD8比值明显降低(P<0.05),Th1和Th2细胞百分率差异无统计学意义(P>0.05),但Th1/Th2比值明显增高(P<0.05);CGN患者CD28⁺、CD4⁺CD28⁺细胞百分率明显减少(P均<0.01)。

结论

CGN患者体内T细胞免疫功能紊乱和活化受限,细胞免疫功能低下,Th1和Th2细胞失衡,通过对CGN患者外周血Th1/Th2及CD28⁺细胞的检测,有助于了解患者的细胞免疫状态,为临床诊断和免疫治疗提供参考依据。

关键词: Th1/Th2细胞, CD28+细胞, 流式细胞术, 慢性肾小球肾炎

Abstract: Objective

To investigate the possible mechanism of cellular immunology in the process of chronic glomerulonephritis (CGN) by the detection of Th1/Th2 ratio and CD28⁺ cell percentages from peripheral blood in patients with CGN.

Methods

Th1/Th2 ratio and CD28⁺ cell percentages were detected by flow cytometry from 50 patients with CGN and 30 healthy subjects (control group). SPSS 16.0 software was used to analyze the results.

Results

Compared with control group, the percentages of CD3⁺CD4⁺ cells and CD4/CD8 ratio of patients with CGN decreased significantly (P<0.05). Although there was no statistical significance in the percentages of Th1 and Th2 cells (P>0.05), yet Th1/Th2 ratio increased significantly (P<0.05). The percentages of CD28⁺ and CD4⁺CD28⁺ cells decreased significantly (P<0.01).

Conclusions

T cell immune function is disorder, and activation is restricted in patients with CGN. Their cellular immune function is low, and there exists imbalance between Th1 and Th2 cells. The detection of Th1/Th2 ratio and percentages of CD28⁺ cells from peripheral blood in patients with CGN is valuable to understand the immune state so as to provide the theoretical basis for immune therapy.

Key words: Th1/Th2 cell, CD28+ cell, Flow cytometry, Chronic glomerulonephritis

中图分类号:

R446.63

周韵娇, 李鹏, 张丽红, 钱英俊, 牛建英. 慢性肾小球肾炎患者外周血Th1/Th2及CD28⁺细胞的变化及其临床意义[J]. 检验医学, 2015, 30(2): 141-144.

ZHOU Yunjiao, LI Peng, ZHANG Lihong, QIAN Yingjun, NIU Jianying. The variation and its clinical significance of Th1/Th2 and CD28⁺ cells from peripheral blood in patients with chronic glomerulonephritis[J]. Laboratory Medicine, 2015, 30(2): 141-144.

图/表 2

参考文献 13

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组别	CD3⁺ (%)	CD3⁺CD4⁺ (%)	CD3⁺CD8⁺ (%)	Th1 (%)	Th2 (%)	CD4/CD8	Th1/Th2
对照组	65.69±6.38	37.43±5.07	23.50±7.24	9.90±3.12	1.45±0.59	1.85±1.00	7.62±2.91
CGN组	63.46±7.37	34.65±5.53^*	27.28±8.81	11.51±3.93	1.30±0.37	1.47±0.72^*	9.67±5.15^*

组别	CD3⁺ (%)	CD3⁺CD4⁺ (%)	CD3⁺CD8⁺ (%)	Th1 (%)	Th2 (%)	CD4/CD8	Th1/Th2
对照组	65.69±6.38	37.43±5.07	23.50±7.24	9.90±3.12	1.45±0.59	1.85±1.00	7.62±2.91
CGN组	63.46±7.37	34.65±5.53^*	27.28±8.81	11.51±3.93	1.30±0.37	1.47±0.72^*	9.67±5.15^*

组别	CD28⁺	CD4⁺CD28⁺	CD8⁺CD28⁺
对照组	56.69±6.82	34.04±4.87	8.35±3.47
CGN组	50.54±5.59^*	30.58±5.30^*	9.50±2.53

组别	CD28⁺	CD4⁺CD28⁺	CD8⁺CD28⁺
对照组	56.69±6.82	34.04±4.87	8.35±3.47
CGN组	50.54±5.59^*	30.58±5.30^*	9.50±2.53

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慢性肾小球肾炎患者外周血Th1/Th2及CD28⁺细胞的变化及其临床意义

The variation and its clinical significance of Th1/Th2 and CD28⁺ cells from peripheral blood in patients with chronic glomerulonephritis

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慢性肾小球肾炎患者外周血Th1/Th2及CD28+细胞的变化及其临床意义

The variation and its clinical significance of Th1/Th2 and CD28+ cells from peripheral blood in patients with chronic glomerulonephritis

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慢性肾小球肾炎患者外周血Th1/Th2及CD28⁺细胞的变化及其临床意义

The variation and its clinical significance of Th1/Th2 and CD28⁺ cells from peripheral blood in patients with chronic glomerulonephritis