上海地区健康成年人外周血T淋巴细胞亚群参考区间调查

doi:10.3969/j.issn.1673-8640.2014.06.010

检验医学 ›› 2014, Vol. 29 ›› Issue (6): 617-622.DOI: 10.3969/j.issn.1673-8640.2014.06.010

上海地区健康成年人外周血T淋巴细胞亚群参考区间调查

董静懿¹,宁小晓¹,王蕾¹,田建辉²

1. 上海中医药大学附属龙华医院检验科,上海 200032;
2. 上海中医药大学附属龙华医院肿瘤科,上海 200032

收稿日期:2013-07-04 出版日期:2014-06-30 发布日期:2014-06-23
通讯作者: 王蕾,联系电话021-64385700-3206。
作者简介:董静懿,女,1968年生,学士,主管技师,主要从事流式细胞学研究。
基金资助:
国家自然科学基金资助项目(81173224);上海中医药大学附属龙华医院龙医学者项目(LHSD-11)

Investigation on the reference ranges of peripheral blood T lymphocyte subsets among healthy adults in Shanghai

DONG Jingyi¹, NING Xiaoxiao¹, WANG Lei¹, TIAN Jianhui²

1. Department of Clinical Laboratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China;
2. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China

Received:2013-07-04 Online:2014-06-30 Published:2014-06-23

摘要/Abstract

摘要： 目的通过对上海地区333名健康成年人外周血T淋巴细胞亚群计数,调查正常人群T淋巴细胞亚群的参考区间,建立95%可信区间的参考区间,并分析和探讨其变化特点,为机体免疫状态的分析和临床诊疗提供参考。方法采用多参数三色免疫荧光标记外周血T淋巴细胞,使用BD FACSCalibur流式细胞仪检测并分析,获得T淋巴细胞亚群的百分比和绝对值。结果女性的CD3⁺ 百分比、CD4⁺ /CD8⁺比值、CD3⁺ CD4⁺ 百分比和CD4⁺CD45RA⁺百分比分别为(72.6%±8.1%)、1.57(0.74~4.70)、(44.8%±8.0%)和(1.71%±6.3%),均高于男性分别为(70.9%±8.5%)、1.43(0.51~4.12)、(40.6%±9.3%)和(15.1%±6.8%)。男性的淋巴细胞绝对值计数、CD8⁺CD28⁺的绝对值计数分别为(2 069.8±474.1)个/μL、(237.5±95.7)个/μL,均高于女性(1 932.8±469.7)个/μL、(218.1±111.1)个/μL。T淋巴细胞亚群的百分比在不同年龄组之间随年龄增大而发生变化,年龄相差越大,差异越大,其中>50岁组与其他组别比较差异有统计学意义。333名健康成年人外周血淋巴细胞绝对值计数为(1 069.1～2 935.6)个/μL,T淋巴细胞亚群百分比计数为:CD3⁺ 细胞55.3%～87.6%,CD3⁺ CD4⁺ 细胞25.2%～57.6%,CD3⁺ CD8⁺细胞15.0%～42.1%,CD4⁺CD25⁺细胞2.5%～10.5%, CD4⁺CD45RA⁺细胞5.0%～27.2 %,CD8⁺CD28⁺细胞4%～19.3%,CD4⁺ /CD8⁺ 比值0.7～2.9。结论健康成年人T淋巴细胞亚群的百分比分布存在性别和年龄差异,由于这种差异不大,可以合并数据建立上海地区健康成年人外周血T淋巴细胞亚群的参考区间。

关键词: 流式细胞术, 正常参考值, T淋巴细胞亚群

Abstract: Objective To establish the reference ranges of peripheral blood T lymphocyte subsets among 333 healthy adults in Shanghai, to analyze and discuss the change characteristics through the establishment of 95% confidence interval, and to provide the reference for immune status analysis and clinical diagnosis and treatment. Methods Multiparameter three-color immunofluorescence was used to stain the peripheral blood T lymphocytes, and BD FACSCalibur flow cytometry was used to acquire and analyze the percentages and absolute counts of T lymphocyte subsets. Results Among females, the CD3⁺ percentage, CD4⁺/CD8⁺ ratio, CD3⁺ CD4⁺ percentage and CD4⁺CD45RA⁺ percentage (72.6%±8.1%), 1.57(0.74-4.70), (44.8%±8.0%) and (1.71%±6.3%) were respectively higher than those among males (70.9%±8.5%), 1.43(0.51-4.12), (40.6%±9.3%) and (15.1%±6.8%). The lymphocyte absolute count and CD8⁺CD28⁺ absolute count in males (2 069.8±474.1) cells/μL and (237.5±95.7) cells/μL were higher than those in females (1 932.8±469.7) cells/μL and (218.1±111.1) cells/μL. The percentage of T lymphocyte subsets increased with the increase of age.There were statistical significances in >50 year-old group with the other groups. The absolute count of 333 healthy adults for peripheral blood T lymphocyte was (1 069.1-2 935.6) cells/μL. The percentages of T lymphocyte subsets were: CD3⁺ 55.3%-87.6%, CD3⁺CD4⁺ 25.2%-57.6%, CD3⁺CD8⁺ 15.0%-42.1%, CD4⁺CD25⁺ 2.5%-10.5%, CD4⁺CD45RA⁺ 5.0%-27.2 % and CD8⁺CD28⁺ 4%-19.3%, and CD4⁺/CD8⁺ ratio was 0.7-2.9. Conclusions There are differences for the percentage distribution of T lymphocyte subsets among healthy adults between sex and age. Since these differences can be ignored, the reference ranges of peripheral blood T lymphocyte subsets among healthy adults could be established in Shanghai.

Key words: Flow cytometry, Normal reference range, T lymphocyte subset

中图分类号:

R446.63

董静懿,宁小晓,王蕾,田建辉. 上海地区健康成年人外周血T淋巴细胞亚群参考区间调查[J]. 检验医学, 2014, 29(6): 617-622.

DONG Jingyi, NING Xiaoxiao, WANG Lei, TIAN Jianhui. Investigation on the reference ranges of peripheral blood T lymphocyte subsets among healthy adults in Shanghai[J]. , 2014, 29(6): 617-622.

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上海地区健康成年人外周血T淋巴细胞亚群参考区间调查

Investigation on the reference ranges of peripheral blood T lymphocyte subsets among healthy adults in Shanghai

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