安图医院尿路感染患者中段尿中分离出的粪肠球菌和屎肠球菌的耐药性分析

doi:10.3969/j.issn.1673-8640.2014.01.009

检验医学 ›› 2014, Vol. 29 ›› Issue (1): 38-41.DOI: 10.3969/j.issn.1673-8640.2014.01.009

安图医院尿路感染患者中段尿中分离出的粪肠球菌和屎肠球菌的耐药性分析

鲍彩丽¹, 范倩燕², 吴迪², 汤园园², 谈秋雯², 张雪莲¹

1.复旦大学生命科学院, 上海 200093;2.安图医院检验科, 上海 200093

收稿日期:2013-05-30 出版日期:2014-01-20 发布日期:2014-01-20
通讯作者: 张雪莲, 联系电话:021-55665073。
作者简介:鲍彩丽, 女, 1981年生, 学士, 技师, 主要从事临床检验工作。

Analysis on the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from the midstream urine of patients with urinary tract infection in Antu Hospital

BAO Caili¹, FAN Qianyan², WU Di², TANG Yuanyuan², TAN Qiuwen², ZHANG Xuelian¹.

1. College of Life Science, Fudan University, Shanghai 200093, China; 2. Department of Clinical Laboratory, Shanghai Antu Hospital, Shanghai 200093, China

Received:2013-05-30 Online:2014-01-20 Published:2014-01-20

摘要/Abstract

摘要： 目的分析安图医院尿路感染患者中段尿中分离出的粪肠球菌和屎肠球菌的耐药性及耐药基因, 为临床用药提供参考。方法选取尿路感染患者中段尿中分离出的粪肠球菌(23株)和屎肠球菌(18株), 采用API进行菌种鉴定, 纸片扩散法进行体外药物敏感性试验, 并采用聚合酶链反应(PCR)对其耐药基因进行检测。结果 23 株粪肠球菌中tetM、ermB、aac(6′)/aph(2′)、ant(6)-Ⅰ和aph(3′)-Ⅲ基因阳性检出率分别为65.2%、82.6%、100.0%、100.0%和100.0%;18株屎肠球菌中tetM、ermB、aac(6′)/aph(2′)、ant(6)-Ⅰ和aph(3′)-Ⅲ基因阳性检出率分别为55.5%、55.5%、100.0%、94.4%和100.0%。粪肠球菌对青霉素、四环素、环丙沙星、左氧氟沙星、红霉素的耐药率>50.0%, 屎肠球菌对青霉素、氨苄西林、环丙沙星、左氧氟沙星、红霉素的耐药率为100.0%。结果临床分离的肠球菌属耐药相关基因携带率很高, 肠球菌属对多种抗菌药物耐药率较高。

关键词: 粪肠球菌, 屎肠球菌, 耐药性, 耐药基因, 抗菌药物

Abstract: Objective To analyze the drug resistance and gene of Enterococcus faecalis and Enterococcus faecium isolated from the midstream urine of patients with urinary tract infection in Antu Hospital, and to provide the reference for clinical medication. Methods Enterococcus faecalis (23 isolates) and Enterococcus faecium (18 isolates) were isolated and collected from the midstream urine of patients with urinary tract infection. The bacteria were identified by API, and drug sensitivity test was done by Kirby-Bauer disk diffusion method. Drug resistance genes were also detected by polymerase chain reaction(PCR). Results The positive rates of tetM, ermB, aac(6′)/aph(2′), ant(6)-Ⅰ and aph(3′)-Ⅲ genes were 65.2%, 82.6%, 100.0%, 100.0% and 100.0% in the 23 isolates of Enterococcus faecalis. In the 18 isolates of Enterococcus faecium, the positive rates of tetM, ermB, aac(6′)/aph(2′), ant(6)-Ⅰ and aph(3′)-Ⅲ genes were 55.5%, 55.5%, 100.0%, 94.4% and 100.0%. The drug resistance rates of Enterococcus faecalis to panicillin, tetracyline, ciprofloxacin, levofloxacin and erythomycin were >50.0%. The drug resistance rates of Enterococcus faecium to penicillin, ampicillin, ciprofloxacin, levofloxacin and erythomycin were 100.0%. Results The positive rate of drug resistance genes is high in Enterococcus isolated from clinic, which has a high multi-drug resistance.

Key words: Enterococcus faecalis, Enterococcus faecium, Drug resistance, Drug resistance gene, Antibacterial drug

中图分类号:

R378.1

鲍彩丽, 范倩燕, 吴迪, 汤园园, 谈秋雯, 张雪莲. 安图医院尿路感染患者中段尿中分离出的粪肠球菌和屎肠球菌的耐药性分析[J]. 检验医学, 2014, 29(1): 38-41.

BAO Caili, FAN Qianyan, WU Di, TANG Yuanyuan, TAN Qiuwen, ZHANG Xuelian.. Analysis on the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from the midstream urine of patients with urinary tract infection in Antu Hospital[J]. , 2014, 29(1): 38-41.

[1]	朱昱蓉, 张丹, 贺雅星, 李晶晶, 黄晶, 李婷, 刘鹏, 刘荣华. 肺炎克雷伯菌临床分离株分子分群和流行病学特征[J]. 检验医学, 2023, 38(5): 435-440.
[2]	方咏梅, 章燕, 徐雪莹, 钟峰. 金黄色葡萄球菌耐药性与耐药基因和毒力基因的相关性[J]. 检验医学, 2023, 38(4): 357-361.
[3]	王佳玮, 朱威南, 陈颖盈, 季萍, 王颖. 血培养分离碳青霉烯类耐药肺炎克雷伯菌临床分布和耐药基因分析[J]. 检验医学, 2023, 38(4): 362-367.
[4]	邓劲, 刘雅, 吴思颖, 廖全凤, 张为利, 肖玉玲, 谢轶, 马莹, 康梅. 抗菌药物体外联合药物敏感性试验方法[J]. 检验医学, 2023, 38(4): 385-393.
[5]	任燕飞, 张敏, 杨涛, 李荣凯, 梁新. 呼吸重症科患者下呼吸道感染病原菌流行病学分析[J]. 检验医学, 2023, 38(2): 157-162.
[6]	邓晨, 戴家泽, 邱丽红, 沈威廷, 蔡木发, 罗文英. 湛江地区高毒力肺炎克雷伯菌分子流行特征[J]. 检验医学, 2023, 38(11): 1026-1031.
[7]	杨思敏, 轩乾坤, 羽晓瑜, 温冬华, 李广波. 国产某品牌大肠埃希菌和肺炎克雷伯菌及3种碳青霉烯耐药基因试剂性能验证[J]. 检验医学, 2023, 38(1): 56-59.
[8]	朱丰村, 曾云祥, 金晓立, 曾添, 黄天鹏, 林枝, 周林双. 204株碳青霉烯类耐药肠杆菌目细菌耐药性分析[J]. 检验医学, 2023, 38(1): 60-65.
[9]	李若梅, 周银娣. 2019—2021年某教学医院无菌体液样本分离真菌临床分布及耐药性变迁[J]. 检验医学, 2022, 37(8): 757-760.
[10]	王建英, 王珊珊, 朱俊, 黄晓春, 万玉香, 刘云. 肺癌患者化疗后肺部感染病原菌分布及耐药性分析[J]. 检验医学, 2022, 37(4): 356-359.
[11]	孙英, 李轶, 闫文娟, 荆楠, 马冰. 河南地区少见CRE耐药性分析及碳青霉烯酶初筛实验的临床应用[J]. 检验医学, 2022, 37(2): 146-149.
[12]	郭兰芳, 郭燕, 郑永贵, 杨洋, 尹丹丹, 施清喻, 胡付品. 氨苄西林预报粪肠球菌和屎肠球菌亚胺培南敏感性的可行性研究[J]. 检验医学, 2022, 37(2): 150-154.
[13]	宋皓月, 舒文. 肉芽肿性乳腺炎患者结核硬脂酸棒状杆菌毒力因子和耐药性分析[J]. 检验医学, 2022, 37(11): 1075-1078.
[14]	张亚茹, 凌丽燕, 陈敏. EUCAST和CLSI微量肉汤稀释法白念珠菌体外药物敏感性试验结果比较[J]. 检验医学, 2022, 37(10): 974-978.
[15]	王婧婧, 吴林清, 陈如寿, 王玉丰. 耐万古霉素肠球菌耐药基因、转座子结构和多位点序列分析[J]. 检验医学, 2021, 36(9): 891-895.

安图医院尿路感染患者中段尿中分离出的粪肠球菌和屎肠球菌的耐药性分析

Analysis on the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from the midstream urine of patients with urinary tract infection in Antu Hospital

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics