关键词: 染色体, G显带, 改良, 外周血淋巴细胞, 胰蛋白酶

Abstract: Objective To investigate the improvement of Giemsa banding technique (G banding technique) for chromosome of peripheral blood lymphocytes, and improve the effects of G banding, save time and further improve the efficiency in the diagnosis of chromosome. Methods For 250 suspected patients, cultivation and smear production of peripheral blood lymphocytes were carried out by routine method. Peripheral blood lymphocytes were digested in 0.05% trypsin working solution for 20s, and stained by Giemsa. The results were compared with the routine banding technique digested in 0.025% trypsin working solution for 5-6 min. Results Specimen by improved method presented clear band and repeated prominent shade band, with 98% of pass and 72.8% of optimal ratio, while the routine method achieved 98% of pass and 44.8% of optimal ratio. Two methods had statistical significance in optimal ratio (P<0.01). Conclusions The application of improved technique not only shortens time for smear production of chromosome and achieves optimal effects, but also saves time for staffs, and shows results as soon as possible, improving efficiency in clinical diagnosis of chromosome diseases. Therefore, this improved method is worthy of publicity.

Key words: Chromosome, G banding, Improvement, Peripheral blood lymphocyte, Trysin