Table of Content

30 June 2019, Volume 34 Issue 6

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Orginal Article

Relationship between serum uric acid and red blood cell parameters in hyperuricemia patients

MA Zhengyao, GUO Wei, PAN Baishen, WANG Beili

2019, 34(6):  486-490.  DOI: 10.3969/j.issn.1673-8640.2019.06.002

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Objective To investigate the relationship between serum uric acid（UA） and red blood cell（RBC） parameters in hyperuricemia（HUA） patients. Methods A total of 1 168 HUA patients（HUA group） and 1 311 healthy subjects（healthy control group） were enrolled. The results of UA,RBC count,hemoglobin（Hb）,mean corpuscular hemoglobin（MCH）,mean corpuscular hemoglobin concentration（MCHC）,red blood cell distribution width（RDW） and mean corpuscular volume（MCV） were collected. All the research subjects were classified into male and female groups,and HUA group was sub-classified according to the quartiles of UA. The relationship betweeen UA and RBC parameters was analyzed by Pearson analysis,multiple binary Logistic analysis and multiple linear regression analysis. Results The levels of UA, MCH,MCHC,RBC count and Hb were higher in HUA group than those in healthy control group for males（P<0.05）. The levels of UA,MCH,RDW,RBC count and Hb were higher in HUA group than those in healthy control group for females（P<0.05）,and the level of MCV was lower（P<0.001）. The increased RBC count was related with the increasing of UA levels for males and females（P<0.001）,and the decreased RDW level was related with the increasing of UA levels for males（P<0.05）. Pearson analysis showed that there were positive correlations between UA and RBC count in males（r=0.119,P=0.002） and in females（r=0.180,P<0.001）. There was a negative correlation between UA and RDW in males（r=-0.017,P=0.005）. Multiple binary Logistic analysis showed that the UA levels of male HUA patients with UA>0.46-0.49 mmol/L and UA>0.49 mmol/L were independent factors for the decreasing of RDW level [odds ratio（OR）=0.773 and 0.690,respectively] and the increasing of RBC count（OR=1.938 and 1.787,respectively）. The UA level of female HUA patients with UA>0.41 mmol/L was the independent factor for the increasing of RBC count（OR=4.578）. Multiple linear regression analysis showed that the level of UA was positively correlated with RBC count in males（B=0.128,P=0.019）. The level of UA was negatively correlated with RDW in males（B=-0.119,P=0.004）. The level of UA was positively correlated with RBC count in females（B=0.296,P<0.001）. Conclusions The increasing of UA level is an independent factor for the increasing of RBC count in patients with HUA.

Role of the determination of statin metabolism-related gene in the treatment of cardiovascular and cerebrovascular diseases

TONG Minghong, DING Hui, JIANG Yinting, SUN Hanxiao, XUAN Binbin, SHENG Huiming

2019, 34(6):  491-497.  DOI: 10.3969/j.issn.1673-8640.2019.06.003

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Objective To study the individualized application of statin metabolism-related genomics in patients with cardiovascular and cerebrovascular diseases,and to investigate its role in blood lipid management. Methods A total of 141 patients with cardiovascular and cerebrovascular diseases（72 cases of coronary heart disease and 69 cases of cerebral infarction） were enrolled,and 70 healthy subjects were enrolled as healthy control group. Serum apolipoprotein A（apo A）,apolipoprotein B（apo B）,apolipoprotein E（apo E）,lipoprotein（a）[Lp（a）],total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol （HDL-C）,free fatty acid（FFA） and the statin gene ABCB1（2677G>T）,ABCB1（3435C>T）and SLCO1B1*5（T>C）single nucleotide polymorphisms（SNP） were determined. Results Serum TG,HDL-C,apo A,Lp（a） and FFA levels in cardiovascular and cerebrovascular disease group had statistical significance compared with healthy control group（P<0.05）. Serum TG,HDL-C,LDL-C,apo A,Lp（a） and FFA levels in coronary heart disease group had statistical significance compared with healthy control group（P<0.05）. Serum TG,HDL-C and Lp（a） levels in cerebral infarction group had statistical significance compared with healthy control group（P<0.05）. Other blood lipid indicators had no statistical significance（P>0.05）. The genotypes and frequency distributions of cardiovascular and cerebrovascular disease group,cerebral infarction group and coronary heart disease group had consistency. ABCB1（2677G>T） G allele frequency was higher than T allele frequency,ABCB1（3435C>T） C allele frequency was higher than T allele frequency,and SLCO1B1*5（T>C） T allele frequency was higher than C allele frequency. After the treatment with statins,serum TG,TC and LDL-C levels were lower than those before treatment（P<0.05）,and serum HDL-C level was higher than that before treatment（P<0.05）. There was statistical significance in the efficiency of statins in patients with different genotypes（P<0.05）. Conclusions There are abnormal blood lipid levels in patients with cardiovascular and cerebrovascular diseases,and there is obvious individual difference for gene polymorphisms in patients with same disease. Pharmacogenomics detection in patients with cardiovascular and cerebrovascular diseases plays a role in guiding the clinical blood lipid-lowering treatment with statins.

Role of HPV DNA typing in the differential diagnosis of cervical lesions and cervical cancer

SHEN Qionghua

2019, 34(6):  498-501.  DOI: 10.3969/j.issn.1673-8640.2019.06.004

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Objective To investigate the role of human papillomavirus（HPV） DNA typing in the differential diagnosis of cervicitis,cervical precancerous lesions and cervical cancer. Methods The clinical data of 524 patients diagnosed with cervical diseases were analyzed retrospectively,and the patients were classified into cervicitis group（240 cases）,cervical intraepithelial neoplasia（CIN）Ⅰ group（132 cases）,CINⅡ group（87 cases）,CINⅢ group（43 cases） and cervical cancer group（22 cases）. The results of HPV DNA typing were analyzed among the 5 groups. Results There was statistical significance in the positive rate of high-risk subtype HPV among the 5 groups（P<0.05）,but there was no statistical significance in the positive rate of low-risk subtype HPV among the 5 groups（P>0.05）. The positive rates of HPV DNA typing showed cervicitis group<CINⅠ group<CINⅡ group<CINⅢ group<cervical cancer group（P<0.05）. Conclusions HPV DNA typing can provide a reference for the differential diagnosis of cervicitis,cervical precancerous lesions and cervical cancer.

Distribution and drug resistance of 354 isolates of Pseudomonas aeruginosa in a hospital of Beijing

MAO Lei, WANG Wei, SU Jianrong

2019, 34(6):  502-505.  DOI: 10.3969/j.issn.1673-8640.2019.06.005

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Objective To analyze the source of Pseudomonas aeruginosa and the drug resistance status for different ages,and to provide a reference for clinical anti-infection treatment. Methods Identification and drug susceptibility analysis for 3 726 clinically isolated bacteria from June 2017 to February 2018 were performed. The patients for the 3 726 clinically isolated bacteria were classified into ≤14-year-old,15-60-year-old and >60-year-old groups. Results A total of 354 isolates of Pseudomonas aeruginosa were isolated from the 3 726 clinical specimens,and the isolation rate was 9.5%. The main types of clinical specimens were sputum（59.9%）,drainage fluid（13.3%）,urine（10.7%）,throat swab（6.2%）,blood（5.1%） and secreta（1.4%）. The departments with high isolation rates were intensive care unit（ICU）（19.4%）,emergency（18.4%） and respiratory medicine（12.7%）. Pseudomonas aeruginosa isolated from ≤14-year-old group were sensitive to amikacin,cefepime and tobramycin. The drug resistance rates of 15-60-year-old and >60-year-old groups were similar,and the drug resistance rates to amikacin were the lowest（8.8% and 11.7%,respectively）,followed by tobramycin,gentamicin and cefepime. Conclusions Pseudomonas aeruginosa are isolated from sputum mainly. The drug resistance rates to amikacin and tobramycin are low. Clinic should choose rational antibiotics according to the results of drug susceptibility analysis.

Distribution and drug resistance of pathogens isolated from blood specimens from 2013 to 2018

YAO Rongfeng, FANG Hui, XU Guoxiang, WU Yazhou1, REN Shengjie, LI Zhi

2019, 34(6):  506-512.  DOI: 10.3969/j.issn.1673-8640.2019.06.006

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Objective To analyze the distribution and drug resistance of pathogens isolated from blood specimens from 2013 to 2018 in Yangpu Hospital of Tongji University,and to provide a reference for clinical anti-infection treatment. Methods The bacteria were identified,and drug susceptibility test was performed by automatic identification system. The distribution and drug resistance characteristics were analyzed. Results A total of 1 803 isolates of pathogens were isolated from 14 085 cases of blood culturing from 2013 to 2018. The positive rate was 12.8%. Among them,Gram-negative bacilli,Gram-positive cocci and fungi accounted for 51.1%,46.4% and 2.4%,respectively. The top 5 pathogens were coagulase-negative Staphylococcus,Escherichia coli,Staphylococcus aureus,Klebsiella pneumoniae and Enterococcus faecalis. Intensive care unit（ICU）（19.3%） had the highest isolation rate of pathogens from blood culturing. The isolation rate of Escherichia coli and Klebsiella pneumoniae from department of surgery were 32.8% and 14.2%,respectively,which were higher than those in internal medicine department（26.8%% and 6.9%） and ICU（16.1% and 6.3%）（P<0.05）. The isolation rate of Escherichia coli from female patients were 33.0%,which was higher than that from male patients（20.7%）（P<0.05）. Imipenem,meropenem,amikacin,cefoperazone/sulbactam,piperacillin/tazobactam maintained good antimicrobial activity against Escherichia coli,Klebsiella pneumoniae,Pseudomonas aeruginosa and Serratia marcescens,and the drug resistance rates were all <15%. The drug resistance rates of Acinetobacter baumannii to most antibiotics were >40%. Staphylococcus was found not to be resistant to linezolid,tigecycline and vancomycin. The drug resistance rate of Enterococcus faecalis to linezolid was 1.6%. The drug resistance rate of Enterococcus faecium to vancomycin was 7.4%. Conclusions The kinds of pathogens isolated from blood culturing are complex. Clinicians should standardize the use of antibiotics according to drug susceptibility test results of positive pathogens from blood culturing.

Distribution and drug resistance analysis of pathogenic bacteria isolated from infectious patients with diabetic foot

LIU Chunlin, XU Bo, CHEN Di, ZHANG Li, WANG Feiying, XU Hongyun, LIU Chang, LÜ Hongling

2019, 34(6):  513-517.  DOI: 10.3969/j.issn.1673-8640.2019.06.007

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Objective To study the distribution and drug resistance of pathogenic bacteria isolated from infectious patients with diabetic foot,and to provide a reference for using antibiotics rationally. Methods A retrospective study was carried out on the pathogenic bacterium identification and drug resistance isolated from the foot ulcers of 267 patients with diabetic foot. Results Among the 267 patients with diabetic foot,292 isolates of pathogenic bacteria were isolated from foot ulcers in 216 patients,and the positive rate was 80.9%（216/267）. There were 160（54.8%） isolates of Gram-negative bacilli,128（43.8%） isolates of Gram-positive cocci and 4（1.4%） isolates of fungi. Mixed infection was found in 46（17.2%） patients. Among the 160 isolates of Gram-negative bacilli,Proteus mirabilis,Escherichia coli,Enterobacter cloacae and Klebsiella pneumoniae were prevalent. Among the 128 isolates of Gram-positive cocci,Staphylococcus aureus,Enterococcus faecalis and Staphylococcus epidermidis were prevalent. Drug susceptibility test showed that carbapenems（imipenem,meropenem and ertapenem）,amikacin,cefepime and beta lactamase inhibitor were the most effective drugs against Gram-negative bacilli,and the drug resistance rates to ampicillin,piperacillin and aztreonam were high. Gram-positive cocci were sensitive to vancomycin,teicoplanin,linezolid,moxifloxacin and rifampicin,and the drug resistance rates to penicillin,erythromycin,clindamycin and tetracycline were high. Methicillin-resistant Staphylococcus aureus accounted for 32.7%（17/52） of Staphylococcus aureus. Escherichia coli and extended-spectrum beta-lactamases（ESBLs）-producing Klebsiella pneumoniae accounted for 44.4%（16/36） and 43.5%（10/23）. Conclusions The distribution of pathogenic bacteria causing diabetic foot infection is widespread. Gram-negative bacilli are the main pathogenic bacteria compared with Gram-positive cocci. A certain proportion of pathogenic bacteria are multi-drug resistant bacteria. Attention should be paid to the pathogenic bacteria and drug susceptibility test in diabetic foot infection patients. The key to the treatment of diabetic foot infection is early application of sensitive antibiotics.

Expressions and roles of serum IL-33 and sST2 in children with systemic lupus erythematosus

WANG Xia, SUO Minghuan, HU Ting, HU Yaozong, WEN Dongmei

2019, 34(6):  518-521.  DOI: 10.3969/j.issn.1673-8640.2019.06.008

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Objective To investigate the expressions and roles of serum interleukin 33（IL-33）and soluble suppression of tumorigenicity 2（sST2） in children with systemic lupus erythematosus（SLE）. Methods Serum levels of IL-33 and sST2 in 32 children with SLE（SLE group） and 50 healthy children（healthy control group） were determined by enzyme-linked immunosorbent assay（ELISA）. The correlations between IL-33,sST2 and systemic lupus erythematosus disease activity index 2000（SLEDAI-2000）,anti-double-stranded DNA（anti-dsDNA） antibody were evaluated. Results Serum levels of IL-33 and sST2 were higher in SLE group than those in healthy control group（P<0.01）. Spearman correlation analysis showed that serum levels of IL-33 and sST2 in children with SLE were positively correlated with SLEDAI-2000（r=0.399 and 0.502,P=0.024 and 0.003,respectively）. Serum levels of IL-33 and sST2 in children with SLE were positively correlated with anti-dsDNA antibody（r=0.395 and 0.529,P=0.025 and 0.002,respectively）. Conclusions Serum IL-33 and sST2 may play roles in the pathogenesis of SLE among children.

Correlations between IL-13,AICDA and TSLP gene loci and IgE in cord blood

TANG Lin, ZHU Chenchen, ZHANG Xiaojing, XIAO Ye, WEI Bing, CAI Mei, TANG Zhenhua

2019, 34(6):  522-525.  DOI: 10.3969/j.issn.1673-8640.2019.06.009

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Objective To investigate the correlations between interleukin 13（IL-13）,activation-induced cytidine deaminase（AICDA） and thymic stromal lymphopoietin（TSLP） gene loci and immunoglobulin E（IgE） in cord blood. Methods Cord blood samples were collected from 779 neonates. Genomic DNA was extracted from cord blood samples,and polymerase chain reaction-ligase detection reaction（PCR-LDR） was used to detect IL-13 gene loci（rs20541,rs1800925,rs848,rs1295686 and rs2069743）,AICDA gene loci（rs2518144,rs17792729,rs2580873,rs2028373 and rs7304723） and TSLP gene loci（rs3806933,rs1545169,rs764917,rs1837253,rs2289276 and rs11466749）. Cord blood IgE level was determined. Results There were correlations between IL-13 gene locus rs1295686（β=0.087,P=0.02）,AICDA gene locus rs2028373（β=-0.082,P=0.02）,TSLP gene loci rs2289276,rs764917 and rs1837253（β=-0.126,-0.083 and 0.188,P=0.01,0.03 and 0.00,respectively） and cord blood IgE. Conclusions IL-13 gene locus rs1295686,AICDA gene locus rs2028373 and TSLP gene loci rs2289276,rs764917 and rs1837253 may be allergy susceptible loci.

Characteristic of Auer rod-like inclusions in the cytoplasm of non-myeloid neoplasm cells

ZHU Jianfeng, GUO Wei, PAN Baishen, WANG Beili

2019, 34(6):  526-529.  DOI: 10.3969/j.issn.1673-8640.2019.06.010

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Objective To report 2 cases of Auer rod-like inclusions in the cytoplasm of non-myeloid neoplasm（acute T lymphoblastic lymphoma/leukemia and splenic lymphoma） cells. To investigate the morphological characteristics of Auer rod and Auer rod-like inclusions. Methods The 2 cases of non-myeloid neoplasm were diagnosed by morphology,immunohistochemical staining,immunophenotype and cytogenetic analysis. Literature review was performed. Auer rod-like inclusions in the cytoplasm of non-myeloid neoplasm cells were evaluated. Results The results of immunohistochemical staining and immunophenotype were compatible with lymphoid neoplasm. For the 2 cases,the morphological characteristics of Auer rod-like inclusions and Auer rod were similar,especially in 1 case of Auer rod-like inclusion appearing in lymphoblast cell,and it was difficult to distinguish Auer rod-like inclusion and Auer rod. Conclusions Auer rod is a cytological marker of myeloid neoplasm,and it is critical in myelodysplastic syndrome（MDS） classification. However,Auer rod could be infrequently found in non-myeloid neoplasm cells,so it should be carefully revaluated not only by morphology,but also by immunohistochemical staining,immunophenotype and cytogenetic analysis.

Changes of biochemical parameters of psoriasis metabolic syndrome patients before and after the treatment with the inhibitor of TNF

CHANG Jinghua, WANG Jianbiao

2019, 34(6):  530-533.  DOI: 10.3969/j.issn.1673-8640.2019.06.011

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Objective To compare the biochemical parameters of psoriasis patients before and after tumor necrosis factor（TNF） inhibitor treatment,and to investigate the possible mechanism of anti-TNF antibody in reducing the risk of metabolic syndrome among psoriasis patients. Methods A retrospective cohort study of psoriasis patients treated with anti-TNF antibody was conducted. A total of 203 psoriasis patients were matched by sex,age,disease duration and PASI score,and 200 healthy subjects were enrolled as healthy control group. Totally,21 biochemical parameters were compared between healthy control group and psoriasis group before and after treatment. Results The levels of high-density lipoprotein cholesterol（HDL-C） and apolipoprotein A（apo A） in psoriasis group were lower than those in healthy control group before treatment（P<0.05）,and the levels were close to those in healthy control group after TNF inhibitor treatment. There was no statistical significance for the other parameters between healthy control group and psoriasis group before and after treatment（P>0.05）. Conclusions Anti-TNF antibody can improve the biochemical parameters related to psoriasis metabolic syndrome. It provides a reference for the treatment of metabolic syndrome caused by psoriasis and the pathogenesis of psoriasis metabolic syndrome.

Genetic diagnosis of spinocerebellar ataxia by PCR amplification and Sanger sequencing

CHEN Jiuming, GU Mingmin, SUN Shunchang

2019, 34(6):  534-538.  DOI: 10.3969/j.issn.1673-8640.2019.06.012

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Objective To investigate the causative mutations in a spinocerebellar ataxia（SCA） pedigree and the types of the SCA pedigree,and to provide a reference for genetic counseling. Methods Genomic DNA was extracted from peripheral blood of 5 subjects,including 3 SCA patients,in the SCA pedigree. Totally,9 sets of primers were designed for the polymerase chain reaction（PCR） amplification of 9 fragments,including SCA1,SCA2,SCA3,SCA6,SCA7,SCA8,SCA12,SCA17 and dentatorubral-pallidoluysian atrophy（DRPLA） gene,respectively. Causative mutations were screened and were validated by Sanger sequencing. Results Repeat lengths of 76,78 and 75,above that of 12-44 CAG triplets reported in healthy people from literatures,were observed in the 3 patients from the SCA3 pedigree. Another member carrying a repeat length of 74 in the pedigree was diagnosed as a causative mutation of the SCA3 gene carrier by Sanger sequencing. Conclusions PCR amplification combined with Sanger sequencing is a convenient and efficient method to identify causative mutations for SCA gene,which can be used for carrier screening and prenatal diagnosis.

Roles of HA,Ⅳ-C,APRI and Fib-4 in the diagnosis of hepatitis B-related liver fibrosis

YU Xiaochun, HU Xujiao, ZHANG Jiaqing, GUO Leilei

2019, 34(6):  539-542.  DOI: 10.3969/j.issn.1673-8640.2019.06.013

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Objective To evaluate the diagnosis efficiency of hyaluronic acid（HA） and Ⅳ-collagen（Ⅳ-C）in patients with liver fibrosis after hepatitis B. Aspartate aminotransferase（AST）/platelet（PLT） ratio（APRI） and fibrosis 4 score（Fib-4）for liver fibrosis after hepatitis B. Methods A total of 320 patients with hepatitis B were enrolled. Their general data,like age and sex,were recorded. The patients were classified according to the Metavir scoring system. The HA and Ⅳ-C levels were detected by chemiluminescence,and APRI and Fib-4 were calculated. Their roles have been evaluated according to receiver operating characteristic（ROC）curve analysis. Results HA,Ⅳ-C,APRI and Fib-4 were positively correlated with the stage of liver fibrosis with statistical significance（P<0.05）,and the areas under curves（AUC） of HA,Ⅳ-C,APRI and Fib-4 were 0.646,0.695,0.743 and 0.701,respectively. Conclusions HA,Ⅳ-C,APRI and Fib-4 have correlations with hepatitis B-related liver fibrosis. They are potential in the non-invasive diagnosis of hepatitis B-related liver fibrosis.

Performance comparison of 4 panels for in vitro drug susceptibility test of Streptococcus pneumoniae

MA Bing, WANG Fangfang, WANG Shanmei, ZHANG Jiangfeng, YAN Wenjuan, JING Nan, LI Yi

2019, 34(6):  547-550.  DOI: 10.3969/j.issn.1673-8640.2019.06.016

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Objective To evaluate the consistency of 4 panels for in vitro drug susceptibility test of Streptococcus pneumoniae. Methods The concentration gradient method was used as a reference method. The in vitro drug susceptibility test was done with the SMIC panels of the American BD company's Phoenix100（BD-SMIC）,the Vitek 2 Compact GP68 panels of French Bio Mérieux（Vitek 2-GP68）,the Streptococcus panels of Zhuhai Deere Bioengineering Co.（DL-STREP） and the Streptococcus panels of Wenzhou Kangtai Biological Technology Co.,Ltd（KT-STREP）. The results were evaluated by 4 parameters,including category agreement（CA）,essential agreement（EA）,very major discrepancy（VMD）and major discrepancy（MD）. Results The MD values of penicillin were influenced by the interpretation criteria. Other drugs（erythromycin,trimethoprim-sulfamethoxazole,cefotaxime,ceftriazone,meropenem,clindamycin,levofloxacin,vancomycin and linezolid） involved in the comparison were acceptable（EA≥90%,CA≥90%,VMD<3% and MD<3%）. Conclusions Except penicillin,the in vitro drug susceptibility test results of the 4 panels are consistent.

Performance evaluation on the rapid determination of thrombus 4 items by magnetic particle chemiluminescence assay

LIU Yanhong, LI Yan, XIE Dongping, DONG Xiaoyu

2019, 34(6):  551-554.  DOI: 10.3969/j.issn.1673-8640.2019.06.017

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Objective To evaluate the role of magnetic particle chemiluminescence assay for thrombus 4 items,including thrombomodulin（TM）,thrombin-antithrombin（TAT） complex,plasmin-α2 plasmin inhibitor complex（PIC） and tissue plasminogen activator-plasminogen activator inhibitor complex（t-PAIC）. Methods TM,TAT,PIC and t-PAIC were determined. Precision,carry-over rate,accuracy,linear range,clinical reportable range and biological reference interval were analyzed. According to the EP guidelines of the Clinical and Laboratory Standards Institute（CLSI）,the performance of magnetic particle chemiluminescence assay for determining thrombus 4 items was evaluated. Results The within-run and between-run precisions [coefficient of variation（CV）] were <10%. The carry-over rate was <1.0%. Linear range analysis showed that a value was 1.00±0.05,and r2 was ≥0.950 0. The clinical reportable range,accuracy and biological reference interval were all qualified. Conclusions Magnetic particle chemiluminescence assay can be used clinically to determine thrombus 4 items.

Application of 5S management mode in an emergency laboratory of hospital

LI Xin, WU Jing, LOU Jiatao

2019, 34(6):  559-562.  DOI: 10.3969/j.issn.1673-8640.2019.06.019

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Objective To evaluate the efficiency of 5S management mode in an emergency laboratory of hospital. Methods 5S practice was carried out in the emergency laboratory of Shanghai Chest Hospital. The difference was evaluated before and after the 5S practice by comparing the number of items in the work area,the number of walking steps,kilometers per year and 5S score. Results After the 5S practice in the emergency laboratory,the number of items in the work area,the number of walking steps and kilometers per year were reduced,and the 5S score was improved. Conclusions After the 5S management mode is carried out in the emergency laboratory,the work environment is improved effectively,the work flow is optimized,the staff initiative is stimulated,and the quality and efficiency are improved.

Research progress of human serum transferrin glycosylation

GUAN Wenqian, GAO Chunfang

2019, 34(6):  563-566.  DOI: 10.3969/j.issn.1673-8640.2019.06.020

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Transferrin is one of high abundance glycoproteins in serum,which has become the research focus because of its special physiological function. Its multiple functions include antimicrobial activity,nourishing cells,growth and differentiation activities as the researches of its function and structure deepened. Transferrin determination has been widely used in the diagnostic evaluation of iron-related disorders. There is a close relationship with the pathophysiologic state of the liver as well. Recently,altered glycosylation of transferrin has been recognized in a variety of diseases,especially in malignancies as with the achievement of glycomics and related technologies. In this review,transferrin and its altered glycosylation as well as its relationship with diseases are introduced.

Research progress on the increasing of plasma squamous cell carcinoma antigen in non-tumor patients

ZHANG Rong, GAN Jiemin, CHEN Weiya, ZHAO Hu

2019, 34(6):  567-570.  DOI: 10.3969/j.issn.1673-8640.2019.06.021

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Squamous cell carcinoma antigen（SCC-Ag） is a kind of tumor-associated protein with very low plasma level in healthy subjects,and plasma SCC-Ag detection plays a role in the diagnosis,treatment monitoring and prognosis judgement of tumor patients. However,in the clinical application process,SCC-Ag in plasma of non-tumor patients could be increased due to a variety of reasons,such as detection methodology,specific physiological and pathological processes,which causes confusion in clinical diagnosis and treatment. In this review,the increasing of SCC-Ag in non-tumor status has been reviewed in order to understand the characteristics of SCC-Ag and SCC-Ag detection results correctly.