Abstract:

Objective To investigate the relationship of nuclear magnetic resonance spectroscopy（NMR） and traditional biochemical determination methods for determining small and dense low-density lipoprotein cholesterol（sd-LDL-C）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）,total cholesterol（TC） and triglyceride（TG）,and to analyze the level of sd-LDL-C in patients with inconsistent LDL-C and low-density lipoprotein particle（LDL-P）. Methods NMR and traditional biochemical determination methods（biochemical analyzer） were used to determine sd-LDL-C,LDL-C,HDL-C,TG and TC levels in 54 patients,and NMR was used to determine LDL-P level. Pearson correlation analysis was used to evaluate the correlation of the 2 methods. Results The r ² values of traditional biochemical determination methods and NMR for sd-LDL-C,LDL-C,HDL-C,TG and TC were 0.640,0.827,0.875,0.996 and 0.928,respectively（P=0.000）. Traditional biochemical determination methods for determining sd-LDL-C level and NMR for determining LDL5-C,LDL4-C+LDL5-C+LDL6-C,LDL5-C+LDL6-C and LDL6-C were positively correlated（r values were 0.326,0.341,0.352 and 0.800,respectively,P<0.05）. NMR for determining sd-LDL-C,LDL-C,HDL-C and TC levels had statistical significance compared with traditional biochemical determination methods（P=0.000）. According to the LDL-C and LDL-P determination results of NMR,the percentiles of 54 samples were calculated. Patients with LDL-C percentile≥LDL-P percentile had higher LDL6-C,sd-LDL-C and LDL6-P levels than those with LDL-C percentile<LDL-P percentile（P<0.05）.Conclusions There is a correlation between NMR and traditional biochemical determination methods. There is a certain difference in sd-LDL-C levels in patients with inconsistent LDL-P and LDL-C.

Key words: Small and dense low-density lipoprotein cholesterol, Blood lipid, Nuclear magnetic resonance spectroscopy, Enzymatic method