Study on the correlation between the levels of serum and urine trace element of chromium with type 2 diabetic nephropathy

doi:10.3969/j.issn.1673-8640.2014.10.009

Abstract

Abstract: Objective To investigate the levels of serum and urine trace element of chromium (Cr) and its correlation with diabetic nephropathy (DN). Methods The study enrolled 30 patients with type 2 diabetes mellitus (T2DM) (T2DM group), 30 patients with DN (DN group) and 30 healthy subjects (healthy control group). Serum and urine Cr³⁺ and Cr⁶⁺ levels were determined by magnetic nanoparticle (Fe₃O₄ @ZrO₂) combining magnetic solid-phase extraction-flame atomic absorption spectrometry. Serum insulin (INS) and C peptide were detected by radioimmunoassay method. Serum total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), fasting blood glucose(FBG), glycosylated hemoglobin A_1c (HbA_1c), creatinine(SCr),urea nitrogen(BUN), cystatin C(Cys C) and microalbumin (mAlb) were detected. The estimated glomerular filtration rate (eGFR) was calculated by modification of diet in renal disease (MDRD). Resutls The level of serum Cr³⁺ in DN group was significantly lower than those in T2DM and healthy control group(P<0.01). However, urine Cr³⁺ level was significantly higher than those in T2DM and healthy control group(P<0.01). The level of serum Cr⁶⁺ in DN group was significantly higher than that in healthy control group (P<0.01), and there was no statistical significance with that in T2DM group (P>0.05). In T2DM group, serum Cr³⁺ level was lower than that in healthy control group (P<0.01), and urine Cr³⁺ and serum Cr⁶⁺ levels were higher than those in healthy control group (P<0.01). Between DN and T2DM groups, the level of serum Cr³⁺ had positive correlation with eGFR(r=0.088, P<0.05; r=0.146, P<0.01), and there were negative correlations with HbA_1c, FBG, Cys C, SCr and mAlb(T2DM group: r=-0.098,-0.103,-0.154,-0.127 and -0.355,P<0.05; DN group: r=-0.106, -0.107, -0.195, -0.150 and -0.447, P<0.05). There was no correlation with TC, TG and BUN(T2DM group: r=0.010, 0.011 and 0.044, P>0.05; DN group: r=0.010, 0.010 and 0.049,P>0.05). Conclusions The level of serum Cr in DN group has varying degrees of reduction, but urine Cr increases. Trace element Cr has correlation with kidney injury in T2DM patients.

Key words: Chromium, Trace element, Diabetic nephropathy, Correlation

CLC Number:

Q581

LIU Junfeng, JU Ping, HUANG Juan.. Study on the correlation between the levels of serum and urine trace element of chromium with type 2 diabetic nephropathy[J]. , 2014, 29(10): 1023-1027.

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