Abstract:

Objective　To investigate the influence of enzymes in human sperm acrosome on male fertility. Methods　According to the World Health Organization standard method,52 cases were enrolled as infertile group,and 12 cases were enrolled as normal control group[sperm density (50-100)×10⁹/L and sperm motility 60%-90%],and semen routine analysis was performed. According to the density of sperm,the infertile group was classified into infertile group B1(<20×10⁹/L),C1(20×10⁹ -40×10⁹/L) and D1(>40×10⁹/L),and according to the motility of sperm,the infertile group was classified into infertile group B2(<30%),C2(30%-50%) and D2(>50%). The positive reaction rate and activity intensity of acrosome enzyme (ACE) were detected by fixed gelatin substrate film technique . The positive reaction rate and activity intensity of hyaluronidase (HYD) were detected by modified sodium hyaluronate-gelatin membrane. Results　There was a significant correlation between the positive reaction rate and activity intensity of ACE with sperm density,sperm motility and sperm deformity rate [correlation coefficients (r) were 0.797,0.867; 0.831,0.860 and -0.625,-0.546,P<0.01]. There was a significant correlation between the positive reaction rate and activity intensity of HYD with sperm density,sperm motility and sperm deformity rate (r were 0.832,0.855; 0.842,0.830 and -0.625,-0.554,P<0.01). The sperm deformity rate in infertile group was higher than that in normal control group (P<0.01). The positive reaction rates and activity intensities of ACE and HYD in infertile group C1,D1 and C2,D2 were significantly lower than those in normal control group (P<0.01). The activity of enzymes increased with the sperm density and motility. Conclusions　The detections of sperm ACE and HYD are effective parameters for the analysis of male fertility.

Key words: Acrosome enzyme, Hyaluronidase, Fertility, Male