Abstract:

Objective　To investigate the accuracy of nucleated red blood cell(NRBC) count determined by SYSMEX XN-1000 automatic hematology analyzer(XN-1000). Methods　A total of 66 peripheral blood samples were collected and determined for NRBC by XN-1000,and the results of NRBC% were compared with those by flow cytometry (FCM) and manual microscopy for the same sample. The manual microscopy was as golden standard. Results　There was a good correlation for NRBC between XN-1000 and microscopy(r=0.927),but the correlation was lower than that between FCM and manual microscopy (r=0.956).The correlation between XN-1000 and FCM was the best (r=0.994). The results of XN-1000 and FCM with manual microscopy had no statistical significance(P>0.05). XN-1000 for NRBC% had a better specificity (95.7%) than FCM. Conclusions　XN-1000 automatic hematology analyzer has good performance for detecting NRBC in peripheral blood rapidly,accurately and effectively.

Key words: Sysmex XN-1000 automatic hematology analyzer, Nucleated red blood cell, Accuracy