Accuracy evaluation of routine measurement procedure for serum homocysteine based on liquid chromatography-tandem mass spectrometry

doi:10.3969/j.issn.1673-8640.2018.08.003

Abstract

Abstract:

Objective To evaluate the accuracy of enzymatic cycling method for serum homocysteine（Hcy） based on isotope-dilution liquid chromatography-tandem mass spectrometry（ID-LC/MS/MS） as a contrast method.Methods A total of 20 fresh serum samples were determined by enzymatic cycling method and ID-LC/MS/MS,and the results were compared. The levels of serum Hcy were adjusted until the results were consistent,according to the results of 5 working calibrations,which were determined by enzymatic cycling method and ID-LC/MS/MS.Results The results of standard reference material（SRM） 1950 determined by ID-LC/MS/MS were consistent with certificate value,and the relative bias was <1.0%. The linear equation of ID-LC/MS/MS and enzymatic cycling method was Y=1.204 1X+0.364 6（r²=0.987 9）,and the average bias was 24.0%. After adjusting,the linear equation was Y=0.988 6X+0.260 3（r²=0.999 7）,and the average bias was -0.09%.Conclusions The levels of serum Hcy determined by ID-LC/MS/MS are accurate,which can be used for the accuracy evaluation of routine measurement procedure for serum Hcy.

Key words: Homocysteine, Isotope-dilution liquid chromatography-tandem mass spectrometry, Enzymatic cycling method, Accuracy evaluation

CLC Number:

R446.1

ZHONG Qiaoling, SUN Ji, WENG Xuqi, FENG Ping, XIE Danyun. Accuracy evaluation of routine measurement procedure for serum homocysteine based on liquid chromatography-tandem mass spectrometry[J]. Laboratory Medicine, 2018, 33(8): 679-682.

Figures/Tables 6

References 14

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批次	实测值（μmol/L）	变异系数（%）	相对偏移（%）
1	8.55	1.65	0.59
2	8.49	1.35	0.12

批次	实测值（μmol/L）	变异系数（%）	相对偏移（%）
1	8.55	1.65	0.59
2	8.49	1.35	0.12

工作校准品编号	酶循环法（μmol/L）	ID-LC/MS/MS（μmol/L）	偏移（%）
Ⅰ	9.05	9.19	-1.58
Ⅱ	17.94	17.78	0.87
Ⅲ	23.02	22.72	1.30
Ⅳ	34.03	34.06	-0.10
Ⅴ	48.42	48.89	-0.96

工作校准品编号	酶循环法（μmol/L）	ID-LC/MS/MS（μmol/L）	偏移（%）
Ⅰ	9.05	9.19	-1.58
Ⅱ	17.94	17.78	0.87
Ⅲ	23.02	22.72	1.30
Ⅳ	34.03	34.06	-0.10
Ⅴ	48.42	48.89	-0.96

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