Application of self-made drying device in the preparation of dried blood filter paper samples

doi:10.3969/j.issn.1673-8640.2021.08.017

Abstract

Abstract:

Objective To investigate the value of self-made drying device in the preparation of dried blood filter paper samples. Methods Totally,50 μL of whole blood were dropped onto the filter papers to make blood spots（the diameter was 12 mm）,and fresh blood spots were dried by natural air drying or by self-made drying device. The changes of drying state of blood spots with time under different experimental conditions were recorded. Liquid chromatography-tandem mass spectrometry（LC-MS/MS）was recruited to determine the contents of 8 kinds of amino acids and 15 kinds of carnitines in the dried blood spots. The results were compared between different punching parts [center and peripheral parts（upper left,upper right,lower left and lower right）] and between different drying methods,and the precision and accuracy were evaluated. Results At room temperature of （23±2）℃ and humidity of （60±5）%,it took 95 and 40 min to dry the blood filter paper samples by natural air drying and by the self-made drying device,respectively. However,at room temperature of （23±2）℃ and humidity of （80±5）%,it took 115 min for both methods to dry the blood filter paper samples completely. Except for octanoyl carnitine（C8）and 3-hydroxypalmitoyl carnitine（C16-OH）in the natural air drying with a difference of -15.51% and -19.23%,respectively,and C16-OH in the self-made drying device with a difference of -16.67%,the difference of results between peripheral and center punching sampling was -14.81%-14.61%. The coefficient of variation（CV）（2.85%-21.98%）and accuracy（97.08%-118.18%）of amino acid and carnitine by different drying methods meet the requirements. Conclusions The self-made drying device can improve the drying efficiency of blood filter paper,and it will not affect the results of amino acid and carnitine,suggesting that it can be used for the preparation of dried blood filter paper.

Key words: Self-made drying device, Dried blood filter paper, Chromatographic effect, Precision, Accuracy

CLC Number:

R446.1

CHEN Yujia, SUN Zhiyong, WANG Shaojing, YANG Jiangtao, WU Benqing. Application of self-made drying device in the preparation of dried blood filter paper samples[J]. Laboratory Medicine, 2021, 36(8): 857-863.

Figures/Tables 7

References 12

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干燥程度	自然晾干	吹干装置干燥
干燥程度	自然晾干	即刻	放置10 min后	放置20 min后	放置30 min后
基本干燥	50	25	25	20	20
完全干燥	95	40	40	30	30

干燥程度	自然晾干	吹干装置干燥
干燥程度	自然晾干	即刻	放置10 min后	放置20 min后	放置30 min后
基本干燥	50	25	25	20	20
完全干燥	95	40	40	30	30

干燥方式	ALA			ARG			CIT
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	389.53±22.93	5.89		76.53±3.69	4.82		24.00±2.02	8.41
静置10 min吹40 min	389.53±22.94	4.93	104.98	76.53±3.70	3.54	102.62	24.00±2.03	8.01	105.88
静置20 min吹40 min	389.53±22.95	6.68	102.14	76.53±3.71	5.57	102.96	24.00±2.04	11.22	104.43
静置30 min吹40 min	389.53±22.96	6.46	108.80	76.53±3.72	3.61	105.36	24.00±2.05	10.15	110.80
静置30 min吹50 min	389.53±22.97	7.02	110.92	76.53±3.73	6.6	109.84	24.00±2.06	10.35	114.13
静置30 min吹70 min	389.53±22.98	10.69	108.88	76.53±3.74	5.52	105.54	24.00±2.07	21.98	116.95
干燥方式	LEU			MET			PHE
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	285.00±18.67	6.55		40.79±2.59	6.36		82.53±6.27	7.60
静置10 min吹40 min	285.00±18.68	5.09	103.38	40.79±2.60	2.85	102.39	82.53±6.28	3.79	104.03
静置20 min吹40 min	285.00±18.69	6.54	102.85	40.79±2.61	4.06	100.96	82.53±6.29	4.56	102.50
静置30 min吹40 min	285.00±18.70	7	107.18	40.79±2.62	6.11	106.93	82.53±6.30	6.19	107.92
静置30 min吹50 min	285.00±18.71	7.24	109.35	40.79±2.63	7.3	110.49	82.53±6.31	7.52	109.43
静置30 min吹70 min	285.00±8.72	9.78	108.36	40.79±2.64	7.33	107.87	82.53±6.32	10.12	109.83
干燥方式	TYR			VAL			C0
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	78.92±4.96	6.28		263.97±14.34	5.43		41.02±2.85	6.94
静置10 min吹40 min	80.26±2.69	3.35	101.70	263.97±14.35	3.06	102.62	41.02±2.86	4.41	105.93
静置20 min吹40 min	79.60±4.25	5.34	100.86	263.97±14.36	5.84	101.29	41.02±2.87	6.23	105.19
静置30 min吹40 min	82.72±4.73	5.72	104.81	263.97±14.37	5.73	105.16	41.02±2.88	6.4	107.41
静置30 min吹50 min	83.63±6.17	7.37	105.97	263.97±14.38	6.56	106.48	41.02±2.89	7.28	109.50
静置30 min吹70 min	86.27±7.41	8.59	109.31	263.97±14.39	9.68	105.76	41.02±2.90	8.31	108.85
干燥方式	C2			C3			C4-OH
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	24.42±1.31	5.38		2.18±0.17	7.89		0.29±0.02	7.63
静置10 min吹40 min	24.42±1.32	4.08	103.76	2.18±0.18	3.99	102.14	0.29±0.03	6.92	105.44
静置20 min吹40 min	24.42±1.33	5.35	101.08	2.18±0.19	4.32	100.05	0.29±0.04	4.13	101.16
静置30 min吹40 min	24.42±1.34	6.47	109.77	2.18±0.20	8.94	110.52	0.29±0.05	7.47	108.27
静置30 min吹50 min	24.42±1.35	7.68	112.05	2.18±0.21	6.24	111.72	0.29±0.06	7.4	106.88
静置30 min吹70 min	24.42±1.36	9.44	109.37	2.18±0.22	11.34	108.43	0.29±0.07	14.09	109.37
干燥方式	C4			C5			C5DC
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	0.26±0.01	4.71		0.17±0.01	5.71		0.13±0.01	3.9
静置10 min吹40 min	0.26±0.02	4.85	104.23	0.17±0.02	5.28	100.5	0.13±0.02	11.85	97.08
静置20 min吹40 min	0.26±0.03	4.47	101.45	0.17±0.03	5.02	100.1	0.13±0.03	5.19	102.29
静置30 min吹40 min	0.26±0.04	3.61	106.62	0.17±0.04	6.33	105.15	0.13±0.04	7.93	98.60
静置30 min吹50 min	0.26±0.05	6.76	107.13	0.17±0.05	8.15	105.25	0.13±0.05	9.07	109.02
静置30 min吹70 min	0.26±0.06	10.18	106.56	0.17±0.06	10.05	107.13	0.13±0.06	8.75	108.13
干燥方式	C6			C8			C10
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	0.10±0.01	6.38		0.22±0.01	6.48		0.36±0.02	6.99
静置10 min吹40 min	0.10±0.02	5.83	103.63	0.22±0.02	3.83	102.53	0.36±0.03	4.41	102.44
静置20 min吹40 min	0.10±0.03	5.49	100.52	0.22±0.03	4.98	102.46	0.36±0.04	4.93	103.10
静置30 min吹40 min	0.10±0.04	8.3	108.46	0.22±0.04	5.77	104.84	0.36±0.05	6.99	106.43
静置30 min吹50 min	0.10±0.05	9.72	103.97	0.22±0.05	9.06	107.14	0.36±0.06	10.05	110.28
静置30 min吹70 min	0.10±0.06	10.84	107.77	0.22±0.06	11.78	107.22	0.36±0.07	14.15	108.59
干燥方式	C12			C14			C16
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	0.10±0.01	7.93		0.08±0.01	6.75		1.33±0.11	7.95
静置10 min吹40 min	0.10±0.02	4.61	99.65	0.08±0.02	6.85	102.74	1.33±0.12	5.30	101.17
静置20 min吹40 min	0.10±0.03	7.8	101.56	0.08±0.03	7.73	105.26	1.33±0.13	5.99	100.00
静置30 min吹40 min	0.10±0.04	8.1	105.90	0.08±0.04	8.55	111.58	1.33±0.14	7.11	110.34
静置30 min吹50 min	0.10±0.05	6.27	109.03	0.08±0.05	8.72	111.79	1.33±0.15	7.74	112.08
静置30 min吹70 min	0.10±0.06	13.7	107.81	0.08±0.06	10.67	109.47	1.33±0.16	11.88	109.38
干燥方式	C16-OH				C18
干燥方式	测定值	CV/%	准确度/%		测定值	CV/%		准确度/%
自然晾干	0.01±0	11.13			0.63±0.05	7.64
静置10 min吹40 min	0.01±1	14.08	100.00		0.63±0.06	5.37		99.63
静置20 min吹40 min	0.01±2	9.61	106.82		0.62±0.02	3.81		99.76
静置30 min吹40 min	0.01±3	9.42	118.18		0.70±0.05	6.5		111.04
静置30 min吹50 min	0.01±4	5.96	118.18		0.70±0.05	7.11		111.79
静置30 min吹70 min	0.01±5	9.42	118.18		0.69±0.07	10.61		109.45

干燥方式	ALA			ARG			CIT
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	389.53±22.93	5.89		76.53±3.69	4.82		24.00±2.02	8.41
静置10 min吹40 min	389.53±22.94	4.93	104.98	76.53±3.70	3.54	102.62	24.00±2.03	8.01	105.88
静置20 min吹40 min	389.53±22.95	6.68	102.14	76.53±3.71	5.57	102.96	24.00±2.04	11.22	104.43
静置30 min吹40 min	389.53±22.96	6.46	108.80	76.53±3.72	3.61	105.36	24.00±2.05	10.15	110.80
静置30 min吹50 min	389.53±22.97	7.02	110.92	76.53±3.73	6.6	109.84	24.00±2.06	10.35	114.13
静置30 min吹70 min	389.53±22.98	10.69	108.88	76.53±3.74	5.52	105.54	24.00±2.07	21.98	116.95
干燥方式	LEU			MET			PHE
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	285.00±18.67	6.55		40.79±2.59	6.36		82.53±6.27	7.60
静置10 min吹40 min	285.00±18.68	5.09	103.38	40.79±2.60	2.85	102.39	82.53±6.28	3.79	104.03
静置20 min吹40 min	285.00±18.69	6.54	102.85	40.79±2.61	4.06	100.96	82.53±6.29	4.56	102.50
静置30 min吹40 min	285.00±18.70	7	107.18	40.79±2.62	6.11	106.93	82.53±6.30	6.19	107.92
静置30 min吹50 min	285.00±18.71	7.24	109.35	40.79±2.63	7.3	110.49	82.53±6.31	7.52	109.43
静置30 min吹70 min	285.00±8.72	9.78	108.36	40.79±2.64	7.33	107.87	82.53±6.32	10.12	109.83
干燥方式	TYR			VAL			C0
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	78.92±4.96	6.28		263.97±14.34	5.43		41.02±2.85	6.94
静置10 min吹40 min	80.26±2.69	3.35	101.70	263.97±14.35	3.06	102.62	41.02±2.86	4.41	105.93
静置20 min吹40 min	79.60±4.25	5.34	100.86	263.97±14.36	5.84	101.29	41.02±2.87	6.23	105.19
静置30 min吹40 min	82.72±4.73	5.72	104.81	263.97±14.37	5.73	105.16	41.02±2.88	6.4	107.41
静置30 min吹50 min	83.63±6.17	7.37	105.97	263.97±14.38	6.56	106.48	41.02±2.89	7.28	109.50
静置30 min吹70 min	86.27±7.41	8.59	109.31	263.97±14.39	9.68	105.76	41.02±2.90	8.31	108.85
干燥方式	C2			C3			C4-OH
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	24.42±1.31	5.38		2.18±0.17	7.89		0.29±0.02	7.63
静置10 min吹40 min	24.42±1.32	4.08	103.76	2.18±0.18	3.99	102.14	0.29±0.03	6.92	105.44
静置20 min吹40 min	24.42±1.33	5.35	101.08	2.18±0.19	4.32	100.05	0.29±0.04	4.13	101.16
静置30 min吹40 min	24.42±1.34	6.47	109.77	2.18±0.20	8.94	110.52	0.29±0.05	7.47	108.27
静置30 min吹50 min	24.42±1.35	7.68	112.05	2.18±0.21	6.24	111.72	0.29±0.06	7.4	106.88
静置30 min吹70 min	24.42±1.36	9.44	109.37	2.18±0.22	11.34	108.43	0.29±0.07	14.09	109.37
干燥方式	C4			C5			C5DC
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	0.26±0.01	4.71		0.17±0.01	5.71		0.13±0.01	3.9
静置10 min吹40 min	0.26±0.02	4.85	104.23	0.17±0.02	5.28	100.5	0.13±0.02	11.85	97.08
静置20 min吹40 min	0.26±0.03	4.47	101.45	0.17±0.03	5.02	100.1	0.13±0.03	5.19	102.29
静置30 min吹40 min	0.26±0.04	3.61	106.62	0.17±0.04	6.33	105.15	0.13±0.04	7.93	98.60
静置30 min吹50 min	0.26±0.05	6.76	107.13	0.17±0.05	8.15	105.25	0.13±0.05	9.07	109.02
静置30 min吹70 min	0.26±0.06	10.18	106.56	0.17±0.06	10.05	107.13	0.13±0.06	8.75	108.13
干燥方式	C6			C8			C10
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	0.10±0.01	6.38		0.22±0.01	6.48		0.36±0.02	6.99
静置10 min吹40 min	0.10±0.02	5.83	103.63	0.22±0.02	3.83	102.53	0.36±0.03	4.41	102.44
静置20 min吹40 min	0.10±0.03	5.49	100.52	0.22±0.03	4.98	102.46	0.36±0.04	4.93	103.10
静置30 min吹40 min	0.10±0.04	8.3	108.46	0.22±0.04	5.77	104.84	0.36±0.05	6.99	106.43
静置30 min吹50 min	0.10±0.05	9.72	103.97	0.22±0.05	9.06	107.14	0.36±0.06	10.05	110.28
静置30 min吹70 min	0.10±0.06	10.84	107.77	0.22±0.06	11.78	107.22	0.36±0.07	14.15	108.59
干燥方式	C12			C14			C16
干燥方式	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%	测定值	CV/%	准确度/%
自然晾干	0.10±0.01	7.93		0.08±0.01	6.75		1.33±0.11	7.95
静置10 min吹40 min	0.10±0.02	4.61	99.65	0.08±0.02	6.85	102.74	1.33±0.12	5.30	101.17
静置20 min吹40 min	0.10±0.03	7.8	101.56	0.08±0.03	7.73	105.26	1.33±0.13	5.99	100.00
静置30 min吹40 min	0.10±0.04	8.1	105.90	0.08±0.04	8.55	111.58	1.33±0.14	7.11	110.34
静置30 min吹50 min	0.10±0.05	6.27	109.03	0.08±0.05	8.72	111.79	1.33±0.15	7.74	112.08
静置30 min吹70 min	0.10±0.06	13.7	107.81	0.08±0.06	10.67	109.47	1.33±0.16	11.88	109.38
干燥方式	C16-OH				C18
干燥方式	测定值	CV/%	准确度/%		测定值	CV/%		准确度/%
自然晾干	0.01±0	11.13			0.63±0.05	7.64
静置10 min吹40 min	0.01±1	14.08	100.00		0.63±0.06	5.37		99.63
静置20 min吹40 min	0.01±2	9.61	106.82		0.62±0.02	3.81		99.76
静置30 min吹40 min	0.01±3	9.42	118.18		0.70±0.05	6.5		111.04
静置30 min吹50 min	0.01±4	5.96	118.18		0.70±0.05	7.11		111.79
静置30 min吹70 min	0.01±5	9.42	118.18		0.69±0.07	10.61		109.45

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