Correlationship research of ALOX12 gene polymorphism with type 2 diabetes mellitus and diabetic nephropathy

doi:10.3969/j.issn.1673-8640.2014.03.005

Abstract

Abstract:

Objective　To investigate the correlation of a single nucleotide polymorphism (SNP) locus rs1126667 (R261Q) in the arachidonate 12-lipoxygenase (ALOX12) gene with type 2 diabetes mellitus(T2DM) and diabetic nephropathy(DN). Methods　The SNP of ALOX12 gene was studied using a case-control method by single base extension reactions and matrix-assisted laser desorption ionization-time of flight mass spectrometry platform. A case-control study enrolled 223 T2DM patients [DN group (134 patients) and T2DM without nephropathy group (89 patients)] and 120 healthy subjects (healthy control group). Results　ALOX12 gene locus rs1126667 was consistent with Hardy-Weinberg equilibrium law,and the frequencies of ALOX12 gene locus rs1126667 G/G,G/A and A/A genotypes in the healthy control group were 26%,47% and 27%. The frequencies in T2DM group were 30%,48% and the 22%,and odd ratios (OR) were 1,0.8 and 0.76. The frequencies in DN group were 31%,45% and 24%,and OR were 1,0.88 and 0.94. The frequencies in T2DM without nephropathy group were 28%,54% and 18%,and OR were 1,1.06 and 0.61. No statistical significance in all groups was found(P>0.05). A allele frequencies in the healthy control group,T2DM group,DN group and T2DM without nephropathy group were 50%,46%,47% and 45% without statistical significance(P>0.05). Conclusions　No correlation of the ALOX12 gene polymorphism with T2DM or DM is found in the northeast Han Chinese.

Key words: Arachidonate 12-lipoxygenase gene, Single nucleotide polymorphism, Type 2 diabetes mellitus, Diabetic nephropathy

CLC Number:

Q503

LI Qi,WANG Yan, QIU Yifan, DONG Ling, CHEN Kelin. Correlationship research of ALOX12 gene polymorphism with type 2 diabetes mellitus and diabetic nephropathy[J]. , 2014, 29(3): 219-225.

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