Abstract:

Objective　To investigate the mechanism of erythrocyte immuno-adhering in the pathogenesis of mesangioproliferative glomerulonephritis(MsPGN). Methods　The erythrocyte immuno-adhering function was measured in 34 children patients with MsPGN. Red blood cell C3b receptor rosette (RCR) and tumor red blood cell rosette (TRR) were determined. The complement receptor type 1(CR1)density-associated genotypes (HH type, HL type and LL type) on erythrocyte were determined by polymerase chain reaction (PCR) amplification with Hind Ⅲ restriction enzyme digestion technique. Results　RCR and TRR of the children patients with MsPGN were lower than those of healthy children (P<0.01). The most CR1 density-associated genotype on erythrocyte of the children patients with MsPGN was HH type, and HH type percentage of the children patients with MsPGN decreased significantly(P<0.05). No LL type was determined. TRR of the children patients with MsPGN was lower than that of healthy children with same genotype(P<0.01). TRR of HH typewas higher than that of HL type (P<0.01). Conclusions　The decreasing activation of erythrocyte CR1 induces low capacities of identifying,adhering,clearing immunocomplex(IC),which lead to the deposit of glomeruli and the proliferation of mesangial cells. The decrease of erythrocyte immuno-adhering function plays an important role in the pathogenesis of MsPGN. The results of CR1 density-associated genotypes are correlated with the ability of erythrocyte immuno-adhering function. There will be the secondary decrease of erythrocyte immuno-adhering function of MsPGN.

Key words: Erythrocyte, Immune function, Complement receptor type 1, Mesangioproliferative glomerulonephritis, Child