Abstract:

Objective To investigate the application value of gamma-interferon inducible protein-10（IP-10）mRNA based polymerase chain reation（PCR） fluorescent probe determination technology（IP-10.TB） in the diagnosis of extrapulmonary tuberculosis.Methods Totally，96 patients with extrapulmonary tuberculosis and 51 patients with nontuberculous lesions（non-tuberculous group） from March to October in 2023 at Nanjing Integrated Traditional Chinese and Western Medicine Hospital of Nanjing University of Traditional Chinese Medicine were enrolled for IP-10.TB determination and interferon gamma release assay（IGRA），and the performance was evaluated，as well as the consistency with Xpert MTB/RIF. Furthermore，the positive determination rates of IP-10.TB and IGRA in different types of extrapulmonary tuberculosis were evaluated.Results Compared with non-tuberculous group（0.0%），the positive determination rate of IP-10.TB in patients with extrapulmonary tuberculosis was higher（85.4%）（χ²=98.518，P<0.001）. Compared with IGRA，there was no statistical significance in the sensitivity and specificity of IP-10.TB（χ²=3.200 and 0.033，P>0.05）. The positive coincidence rate with Xpert MTB/RIF was 96.3%，the negative coincidence rate was 50.0%，and the total coincidence rate was 89.1%. There was no statistical significance in the positive determination rate of IP-10.TB in 2 different types of extrapulmonary tuberculosis，namely，lymphoid tuberculosis and bone and joint tuberculosis（χ²=0.156，P=0.693）.Conclusions IP-10.TB is a method with high sensitivity and high specificity in the diagnosis of extrapulmonary tuberculosis，which can be used for rapid diagnosis of different types of extrapulmonary tuberculosis.

Key words: Gamma-interferon inducible protein-10, Interferon gamma release assay, Tuberculosis, Extrapulmonary tuberculosis, Diagnosis