Correlation between biochemical parameters and apolipoprotein E genotypes among inpatients with late-onset Alzheimer’s disease in Beijing

doi:10.3969/j.issn.1673-8640.2020.01.001

Abstract

Abstract:

Objective To investigate the correlation between the gene polymorphism of apolipoprotein E（apo E） and blood lipid levels in late-onset Alzheimer’s disease（LOAD） patients. Methods The apo E gene polymorphism was determined by gene chip in 150 LOAD patients and 150 healthy subjects. Total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,small and dense low-density lipoprotein cholesterol（sd-LDL-C）,high-density lipoprotein cholesterol（HDL-C）,apolipoprotein B（apo B） and lipoprotein （a）[Lp（a）] levels were also determined. Multivariate unconditional Logistic regression analysis was used to screen the related risk factors of LOAD. Results The frequencies of E2/3 and E3/3 genotypes in LOAD group were lower than those in healthy control group（P<0.05）,and the frequencies of E3/4 and E2/4 genotypes were higher than those in healthy control group（P<0.01）. The frequency of ε3 allele in LOAD group was lower than that in healthy control group（P<0.05）,and the frequency of ε4 allele in LOAD group was higher than that in healthy control group（P<0.01）. Compared with healthy control group,the levels of TC,LDL-C and sd-LDL-C were increased（P<0.01）,while HDL-C was decreased（P<0.01） in LOAD group. There was no statistical significance in the other blood lipids（P>0.05）. The levels of TC and LDL-C were gradually increased in LOAD patients with the phenotypes of ε2,ε3 and ε4（P<0.05）. The LDL-C level of ε4 phenotype was higher than that of ε2 phenotype in healthy control group（P<0.05）. The apo E ε4 allele and LDL-C were the risk factors for LOAD,the odds ratios（OR） were 14.454 and 5.824,and 95% confidence intervals（CI） were 5.793-16.368 and 2.582-7.973,respectively. HDL-C was the protective factor for LOAD（OR=0.020,95% CI 0.006-0.352）. Conclusions The apo E gene polymorphism is closely related to lipid metabolism,and the apo E ε4 allele may be one of the important genetic factors in the pathogenesis of LOAD.

Key words: Apolipoprotein E, Gene polymorphism, Alzheimer’s disease, Blood lipid

CLC Number:

R446.1

HONG Ping, WANG Peichang. Correlation between biochemical parameters and apolipoprotein E genotypes among inpatients with late-onset Alzheimer’s disease in Beijing[J]. Laboratory Medicine, 2020, 35(1): 1-5.

Figures/Tables 5

References 18

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组别	例数	基因型						等位基因
组别	例数	E2/2	E2/3	E3/3	E3/4	E2/4	E4/4	ε2	ε3	ε4
AD组	150	8 （5.33）	18（12.00）	21（14.00）	61（40.67）	23（15.33）	19（12.67）	57（19.00）	121（40.33）	122（40.67）
正常对照组	150	0 （0）	32（21.33）	108（72.00）	8 （5.33）	2 （1.33）	0 （0）	34（11.33）	256（85.33）	10 （3.33）
χ²值		6.29	4.06	100.58	50.89	17.46	18.21	6.27	128.15	119.67
P值		>0.05	<0.05	<0.05	<0.01	<0.01	>0.05	>0.05	<0.05	<0.01

组别	例数	基因型						等位基因
组别	例数	E2/2	E2/3	E3/3	E3/4	E2/4	E4/4	ε2	ε3	ε4
AD组	150	8 （5.33）	18（12.00）	21（14.00）	61（40.67）	23（15.33）	19（12.67）	57（19.00）	121（40.33）	122（40.67）
正常对照组	150	0 （0）	32（21.33）	108（72.00）	8 （5.33）	2 （1.33）	0 （0）	34（11.33）	256（85.33）	10 （3.33）
χ²值		6.29	4.06	100.58	50.89	17.46	18.21	6.27	128.15	119.67
P值		>0.05	<0.05	<0.05	<0.01	<0.01	>0.05	>0.05	<0.05	<0.01

组别	例数	TC/ （mmol/L）	TG/ （mmol/L）	LDL-C/ （mmol/L）	sd-LDL-C/ （mmol/L）	HDL-C/ （mmol/L）	apo A/ （g/L）	apo B/ （g/L）	Lp（a）/（mg/L）
AD组	150	4.53±0.85	1.35±0.13	3.57±0.33	1.13±0.11	1.14±0.11	1.27±0.16	1.05±0.11	282.63±199.47
正常对照组	150	3.76±0.87	1.32±0.15	2.38±0.19	0.78±0.05	1.62±0.27	1.35±0.15	0.98±0.08	255.41±183.34
t值		4.57	-0.04	4.63	4.51	-4.09	-0.04	0.46	0.23
P值		<0.01	>0.05	<0.01	<0.01	<0.01	>0.05	>0.05	>0.05

组别	例数	TC/ （mmol/L）	TG/ （mmol/L）	LDL-C/ （mmol/L）	sd-LDL-C/ （mmol/L）	HDL-C/ （mmol/L）	apo A/ （g/L）	apo B/ （g/L）	Lp（a）/（mg/L）
AD组	150	4.53±0.85	1.35±0.13	3.57±0.33	1.13±0.11	1.14±0.11	1.27±0.16	1.05±0.11	282.63±199.47
正常对照组	150	3.76±0.87	1.32±0.15	2.38±0.19	0.78±0.05	1.62±0.27	1.35±0.15	0.98±0.08	255.41±183.34
t值		4.57	-0.04	4.63	4.51	-4.09	-0.04	0.46	0.23
P值		<0.01	>0.05	<0.01	<0.01	<0.01	>0.05	>0.05	>0.05

apo E 表型	例数	TC/ （mmol/L）	TG/ （mmol/L）	LDL-C/ （mmol/L）	sd-LDL-C/ （mmol/L）	HDL-C/ （mmol/L）	apo A/ （g/L）	apo B/ （g/L）	Lp（a）/ （mg/L）
ε2	57	3.45±0.31	2.53±0.51	3.02±0.32	1.21±0.12	1.08±0.11	1.22±0.13	1.08±0.10	297.63±193.58
ε3	121	4.18±0.71^*	2.28±0.47	3.61±0.41^*	0.98±0.09	1.21±0.12	1.31±0.15	1.02±0.09	275.32±179.87
ε4	122	4.89±0.52^*#	2.49±0.61	4.08±0.28^*#	1.16±0.10	1.12±0.11	1.29±0.16	1.04±0.11	287.65±189.43