Abstract:

Objective To investigate the application significance of peripheral blood lymphocyte immunophenotype in cutaneous T-cell lymphoma. Methods Peripheral blood lymphocyte immunophenotype was analyzed by flow cytometry and morphology in 52 patients diagnosed as skin disorders [31 cases of cutaneous T-cell lymphoma（malignant skin disorder group）and 21 cases of inflammatory skin disorders（benign skin disorder group）]. The characteristics of lymphocyte subset,immunophenotype and morphology between benign and malignant skin disorder groups were compared. Results CD4/CD8 double negative T lymphocyte in malignant group was significantly higher than that in benign group （P < 0.05）,and B lymphocyte percentage in malignant group was significantly lower than that in benign group （P < 0.05）. In malignant group,T lymphocyte percentage,CD4/CD8 ratio and CD4/CD8 double negative T lymphocyte percentage in Sézary syndrome subgroup were higher than those in benign group and other malignant skin disorder subgroups （P < 0.05）,meanwhile B lymphocyte and natural killer（NK） cell percentage decreased （P < 0.05）. There were 14 abnormal cases of malignant group（45.1%） and 5 abnormal cases of benign group（23.8%） for immunophenotype. There were 15 abnormal cases of malignant group（48.4%） and 6 abnormal cases of benign group（28.6%）for morphology. Conclusions Peripheral blood lymphocyte immunophenotype can provide a reference for the diagnosis of cutaneous T-cell lymphoma,and it is helpful with the differential diagnosis.

Key words: Cutaneous T-cell lymphoma, Lymphocyte, Immunophenotype