Comparison and application of low platelet counts by CD61 immunological, optical, electrical impedance methods of CELL-DYN Sapphire hematology analyzer

doi:10.3969/j.issn.1673-8640.2015.03.018

Abstract

Abstract: Objective

To evaluate and compare the advantages and disadvantages of 3 detection methods of CELL-DYN Sapphire hematology analyzer in the aspect of low platelet (PLT) detection.

Methods

A total of 100 patients whose platelet <50×10⁹/L caused by basis hematonosis or after chemotherapy were enrolled. PLT counts were determined by electrical impedance method (IPLT), optical method (OPLP) and CD61 immunological method (CD61-PLT). The results were analyzed comparatively with those of manual microscopy. Variance analysis, Passing-Bablok regression analysis and Bland-Altman bias analysis were performed by SPSS 19.0 and MedCalc V12.7.2.0 softwares statistically.

Results

ANOVA analysis showed that there was statistical significance for IPLT and OPLT with manual method (MPLT). (P=0.00, P=0.002). CD61-PLT and MPLT had no statistical significance (P=0.915). OPLT and CD61-PLT had good correlation with MPLT without statistical significance[slope 1.0, 95% confidence interval (CI):0.95-1.06, r=0.946 and slope 1.0, 95%CI: 0.99-1.01, r=0.998]. IPLT had poor correlation with MPLT with statistical significantce (slope 1.27, 95%CI: 1.10-1.44, r=0.845). According to variance analysis, the values of IPLT and OPLT were higher than those of MPLT (mean deviations were 6.3 and 1.3). CD61-PLT and MPLT had no significant difference(mean deviation was -0.02).

Conclusions

IPLT has significantly statistical difference with MPLT for low PLT determination, with poor correlation, and the result has a significant upward deviation. The means of OPLT and MPLT have statistical significance with good correlation, but the result has a still small upward deviation. The CD61-PLT and MPLT have no obvious difference with a good correlation, and the results are not significantly different. Therefore, it is recommended that we can use CD61-PLT as a new reference method and OPLT and IPLT as alternative methods.

Key words: Low platelet, CD61 immunological method, Optical method, Electrical impedance method, CD-SAPPHIRE, Prophylactic platelet transfusion

CLC Number:

R446.11

ZHANG Chi, ZHANG Hongbo. Comparison and application of low platelet counts by CD61 immunological, optical, electrical impedance methods of CELL-DYN Sapphire hematology analyzer[J]. Laboratory Medicine, 2015, 30(3): 274-279.

Figures/Tables 8

项目	低值			中值			高值
项目	电阻抗法	光学法	CD61免疫学法	电阻抗法	光学法	CD61免疫学法	电阻抗法	光学法	CD61免疫学法
$x -$	31.10	25.40	24.4	256.10	254.00	255.50	639.8	633.10	636.10
s	0.99	0.97	0.84	4.56	3.59	2.07	6.01	5.45	2.47
CV(%)	3.20	3.80	3.46	1.78	1.41	0.81	0.94	0.86	0.39

项目	低值			中值			高值
项目	电阻抗法	光学法	CD61免疫学法	电阻抗法	光学法	CD61免疫学法	电阻抗法	光学法	CD61免疫学法
$x -$	31.10	25.40	24.4	256.10	254.00	255.50	639.8	633.10	636.10
s	0.99	0.97	0.84	4.56	3.59	2.07	6.01	5.45	2.47
CV(%)	3.20	3.80	3.46	1.78	1.41	0.81	0.94	0.86	0.39

项目	电阻抗法	光学法	CD61免疫学法PLT
N	82	100	100
均值	35.11	26.45	25.12
s	13.68	13.06	12.72
MPLT均值	28.84	25.14	25.14
P值	0.706	0.755	0.801
P值*	0.00	0.002	0.915
差异均值	6.3	1.3	-0.02
差异均值s	7.2	4.1	0.71
差异均值95%CI	5.17~8.18	0.47~2.13	-0.12~0.16
r值	0.845	0.946	0.998
截距	-1.07	0.65	-0.15
截距95%CI	-4.98~3.30	-0.40~1.66	-0.38~0.15
斜率	1.27	1.00	1.00
斜率95%CI	1.10~1.44	0.95~1.06	0.99~1.01
回归方程	Y=-1.066 7+1.266 7X	Y=0.650 0+1.000 0X	Y=-0.150 0+1.000 0X

PLT阈值 (×10⁹/L)	例数	输注不足 [例(%)]	PLT正确输注(例)	输注过度 [例(%)]
5	2	2(100%)	0	0(0)
10	3	3(100)	0	0(0)
20	24	19(79.2)	5	0(0)
50	80	8(10.0)^*	63	9(11.3)

PLT阈值 (×10⁹/L)	例数	输注不足 [例(%)]	PLT正确输注(例)	输注过度 [例(%)]
5	7	2(28.6)	5	0(0)
10	16	4(25.0)	12	0(0)
20	42	10(23.8)	30	2(4.8)
50	98	1(1.0)^*	91	6(6.1)

PLT阈值 (×10⁹/L)	例数	输注不足 [例(%)]	PLT正确输注(例)
5	7	0(0)	7
10	16	0(0)	16
20	42	2(4.8)	40
50	98	0(0)	98

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