Distribution of 16S rRNA methylase genes in ESBLs-producing Enterobacteriaceae

doi:10.3969/j.issn.1673-8640.2013.09.010

Abstract

Abstract:

Objective To investigate the distribution of 16S rRNA methylase genes in extended-spectrum beta-lactamases (ESBLs)-producing Gram-negative Enterobacteriaceae. Methods A total of 70 isolates of Gram-negative Enterobacteriaceae were identified by VITEK-32 automated microbial system. The Kirby-Bauer method was used to detect ESBLs. Polymerase chain reaction(PCR) was used to detect 6 kinds of 16S rRNA methylase genes including armA, rmtA, rmtB, rmtC, rmtD and npmA. DNA sequencing was used to detect the positive productions of PCR with GenBank as control. Results From the 70 isolates of ESBLs-producing Gram-negative Enterobacteriaceae, 9 isolates carried 16S rRNA methylase genes, including 5 for armA gene, 4 for rmtB gene and 2 for both armA and rmtB genes, and no rmtA, rmtC, rmtD or npmA gene was detected. Conclusions The distribution of 16S rRNA methylase genes is various in the hospitals from different regions.

Key words: 16S rRNA methylase, Drug resistance gene, Gram-negative Enterobacteriaceae

CLC Number:

R378.2

GAO Hui,WANG Yang,HUANG Yunkun, ZHU Wenmei, WANG Jia, YAO Yao. Distribution of 16S rRNA methylase genes in ESBLs-producing Enterobacteriaceae[J]. , 2013, 28(9): 775-779.

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