Research on 16S rRNA methylase in aminoglycoside-resistant Enterobacteriaceae

doi:10.3969/j.issn.1673-8640.2014.05.022

Abstract

Abstract: Objective To investigate the molecular epidemiological characterization and the drug resistance and prevalence mechanism of 16S rRNA methylase in aminoglycoside-resistant Enterobacteriaceae isolated clinically. Methods Gentamicin-and or amikacin-resistant Enterobacteriaceae were screened by disc diffusion method. 16S rRNA methylase genes, aminoglycoside modification enzyme genes and beta-lactamase genes were amplified by polymerase chain reaction(PCR). The conjugal transfer of aminoglycoside-resistant determination was performed. Pulsed-field gel electrophoresis (PFGE) was carried out to analyze genotyping. Results A total of 16 armA gene and 22 rmtB gene 16S rRNA methylase positive isolates were identified. Plasmid conjugation experiments were successful with 30 armA- or rmtB-positive isolates. bla_CTX-M-14, bla_TEM-1 and bla_SHV-12 co-transferred with armA or rmtB were transferred to 11, 20 and 7 isolates. Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae were divided into 4, 21 and 1 PFGE patterns. Conclusions armA and rmtB are the main types of 16S rRNA methylase in Enterobacteriaceae isolated in our hospital, and rmtB is more prevalent than armA. This 16S rRNA methylase could mediate high-level resistance to aminoglycoside, and the encoding genes are located on plasmid that could transfer among different species. Beta-lactamase genes and fluoroquinolone resistance could be cotransferred with armA or rmtB gene.

Key words: 16S rRNA methylase, Enterobacteriaceae, Aminoglycoside drug

CLC Number:

R378.2

WU Qiong, HAN Lizhong, SUN Jingyong, NI Yuxing, CHEN Min. Research on 16S rRNA methylase in aminoglycoside-resistant Enterobacteriaceae[J]. , 2014, 29(5): 528-534.

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