Efficiency evaluation of carbapenem inactivation method for screening carbapenemase-producing Enterobacteriaceae

doi:10.3969/j.issn.1673-8640.2017.07.017

Abstract

Abstract:

Objective To evaluate the efficiency of carbapenem inactivation method （CIM）for screening carbapenemase-producing Enterobacteriaceae. Methods The results of polymerase chain reaction（PCR）and DNA sequencing for determining carbapenemase genes were used as gold standard. A total of 154 clinical isolates of Enterobacteriaceae were determined by CIM,and the results were compared with those of modified Hodge test. Results The consistency rate,specificity and sensitivity of CIM were 99.4%,96.6% and 100.0%,respectively. The consistency rate,specificity and sensitivity of modified Hodge test were 98.7%,93.1% and 100.0%,respectively. Conclusions In comparison to modified Hodge test,CIM shows high consistency rate and specificity. The observation of CIM results is easier than that of modified Hodge test. Therefore,CIM is suitable for using in all level microbiological laboratories.

Key words: Carbapenem inactivation method, Modified Hodge test, Enterobacteriaceae, Carbapenemase

CLC Number:

R446.1

YU Jiajia, LIU Ying, YU Jing, LI Yuanrui, LIU Jingxian. Efficiency evaluation of carbapenem inactivation method for screening carbapenemase-producing Enterobacteriaceae[J]. Laboratory Medicine, 2017, 32(7): 628-632.

Figures/Tables 5

References 19

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碳青霉烯酶基因	引物序列	扩增产物片段长度（bp）
KPC	F: GCTACACCTAGCTCCACCTTC R: ACAGTGGTTGGTAATCCATGC	989
GES	F: AGCGACAATGGGGCTACTAAC R: GTGTAATAACTTGACCGACAGAGG	465
IMP	F: CTACCGCAGCAGAGTCTTTG R: AACCAGTTTTGCCTTACCAT	587
VIM	F: AGTGGTGAGTATCCGACAG R: ATGAAAGTGCGTGGAGAC	261
NDM-1	F: GAGCACCGCATTAGCCGCTG R: GCTATCGGGGGCGGAATGG	727
OXA-48	F: TTGGTGGCATCGATTATCGG R: GAGCACTTCTTTTGTGATGGC	744

碳青霉烯酶基因	引物序列	扩增产物片段长度（bp）
KPC	F: GCTACACCTAGCTCCACCTTC R: ACAGTGGTTGGTAATCCATGC	989
GES	F: AGCGACAATGGGGCTACTAAC R: GTGTAATAACTTGACCGACAGAGG	465
IMP	F: CTACCGCAGCAGAGTCTTTG R: AACCAGTTTTGCCTTACCAT	587
VIM	F: AGTGGTGAGTATCCGACAG R: ATGAAAGTGCGTGGAGAC	261
NDM-1	F: GAGCACCGCATTAGCCGCTG R: GCTATCGGGGGCGGAATGG	727
OXA-48	F: TTGGTGGCATCGATTATCGG R: GAGCACTTCTTTTGTGATGGC	744

细菌碳青霉烯酶基因型	改良Hodge试验		CIM
细菌碳青霉烯酶基因型	阳性	阴性	阳性	阴性
肺炎克雷伯菌
KPC-2	69^*	0	69	0
NDM-1	22	0	22	0
IMP-1	3	0	3	0
IMP-4	1	0	1	0
阴性	1	14	1	14
产气肠杆菌
KPC-2	16	0	16	0
阴性	0	1	0	1
大肠埃希菌
NDM-1	3	0	3	0
KPC-2	1	0	1	0
阴性	1^*	6	0	7
阴沟肠杆菌
VIM-1及VIM-2	3	0	3	0
IMP	1	0	1	0
NDM-1	1	0	1	0
阴性	0	3	0	3
弗氏枸橼酸杆菌
KPC-2	2	0	2	0
KPC-2及IMP-1	1	0	1	0
阴性	0	1	0	1
产酸克雷伯菌
KPC-2及IMP-1	1	0	1	0
阴性	0	1	0	1
解鸟氨酸拉乌尔菌
IMP-1	1	0	1	0
阴性	0	1	0	1
合计
阳性	125	0	125	0
阴性	2	27	1	28

细菌碳青霉烯酶基因型	改良Hodge试验		CIM
细菌碳青霉烯酶基因型	阳性	阴性	阳性	阴性
肺炎克雷伯菌
KPC-2	69^*	0	69	0
NDM-1	22	0	22	0
IMP-1	3	0	3	0
IMP-4	1	0	1	0
阴性	1	14	1	14
产气肠杆菌
KPC-2	16	0	16	0
阴性	0	1	0	1
大肠埃希菌
NDM-1	3	0	3	0
KPC-2	1	0	1	0
阴性	1^*	6	0	7
阴沟肠杆菌
VIM-1及VIM-2	3	0	3	0
IMP	1	0	1	0
NDM-1	1	0	1	0
阴性	0	3	0	3
弗氏枸橼酸杆菌
KPC-2	2	0	2	0
KPC-2及IMP-1	1	0	1	0
阴性	0	1	0	1
产酸克雷伯菌
KPC-2及IMP-1	1	0	1	0
阴性	0	1	0	1
解鸟氨酸拉乌尔菌
IMP-1	1	0	1	0
阴性	0	1	0	1
合计
阳性	125	0	125	0
阴性	2	27	1	28

碳青霉烯酶基因检测	改良Hodge试验		合计
碳青霉烯酶基因检测	阳性	阴性	合计
阳性	125	0	125
阴性	2	27	29
合计	127	27	154