Efficacy evaluation and application value of screening carbapenemase in enterobacteriaceae by mCIM and eCIM

doi:10.3969/j.issn.1673-8640.2021.02.011

Abstract

Abstract:

Objective To investigate the capacity and application value of modified carbapenem inactivation method（mCIM）and ethylene diamine tetraacetic acid-carbapenem inactivation method（eCIM）for phenotypic detection of carbapenemase-producing Enterobacteriaceae. Methods A total of 117 strains of carbapenem-resistant Enterobacteriaceae（CRE）（experimental group）and 50 strains of carbapenem-sensitive Enterobacteriaceae （control group）were collected. Phenotypic screening was performed by modified Hodge test（MHT）,mCIM and eCIM in both groups,respectively. Common carbapenem-resistant genes were detected by polymerase chain reaction（PCR）. The consistency of phenotypic screening and gene examinations was analyzed statistically. Results When the detection result of carbapenem-resistant genes was taken as golden standard,the sensitivity of MHT for screening carbapenemase was 87.0%,and the specificity was 100.0%;both sensitivity and specificity of mCIM were 100.0%;the sensitivity and specificity of eCIM were 82.4% and 100.0% respectively. Conclusion mCIM is more sensitive than MHT in screening carbapenemase. The combined detection of mCIM and eCIM can effectively detect carbapenemase and distinguish the type of carbapenemase,which can play a guiding role in clinical medication.

Key words: Carbapenem-resistant Enterobacteriaceae, Carbapenemase, Modified Hodge test, Modified carbapenem inactivation method, ethylene diamine tetraacetic acid-carbapenem inactivation method

CLC Number:

R378.2

YIN Juan, WANG Yingchao, SUI Yang, JIANG Chun, SHI Yunqi, ZHU Chaowang. Efficacy evaluation and application value of screening carbapenemase in enterobacteriaceae by mCIM and eCIM[J]. Laboratory Medicine, 2021, 36(2): 177-180.

Figures/Tables 4

References 12

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引物名称	引物序列（5´~3´）	退火温度/ ℃	产物长度/bp
bla_KPC-F	GCTACACCTAGCTCCACCTTC	59	989
bla_KPC-R	ACAGTGGTTGGTAATCCATGC
bla_VIM-F	CAGATTGCCGATGGTGTTTGG	57	523
bla_VIM-R	AGGTGGGCCATTCAGCCAGA
bla_IMP-F	CTACCGCAGCAGAGTCTTTG	57	587
bla_IMP-R	AACCAGTTTTGCCTTACCAT
bla_NDM-F	GGTTTGGCGATCTGGTTTTC	60	621
bla_NDM-R	CGGAATGGCTCATCACGATC
bla_OXA-48-F	TTGGTGGCATCGATTATCGG	56	744
bla_OXA-48-R	GAGCACTTCTTTTGTGATGGC

引物名称	引物序列（5´~3´）	退火温度/ ℃	产物长度/bp
bla_KPC-F	GCTACACCTAGCTCCACCTTC	59	989
bla_KPC-R	ACAGTGGTTGGTAATCCATGC
bla_VIM-F	CAGATTGCCGATGGTGTTTGG	57	523
bla_VIM-R	AGGTGGGCCATTCAGCCAGA
bla_IMP-F	CTACCGCAGCAGAGTCTTTG	57	587
bla_IMP-R	AACCAGTTTTGCCTTACCAT
bla_NDM-F	GGTTTGGCGATCTGGTTTTC	60	621
bla_NDM-R	CGGAATGGCTCATCACGATC
bla_OXA-48-F	TTGGTGGCATCGATTATCGG	56	744
bla_OXA-48-R	GAGCACTTCTTTTGTGATGGC

细菌名称	产酶菌株数	KPC	NDM	IMP	KPC+IMP	MHT（+）	mCIM（+）	eCIM（+）
肺炎克雷伯菌	81	79	0	1	1	81	81	0
大肠埃希菌	12	3	9	0	0	3^①	12	9^②
产气肠杆菌	6	5	1	0	0	5^①	6	1^②
阴沟肠杆菌	4	1	3	0	0	1^①	4	3^②
产酸克雷伯菌	3	2	0	1	0	3	3	0
弗劳地枸橼酸杆菌	1	0	1	0	0	0	1	1
科泽枸橼酸杆菌	1	1	0	0	0	1	1	0
总计	108	91	14	2	1	94	108	14

细菌名称	产酶菌株数	KPC	NDM	IMP	KPC+IMP	MHT（+）	mCIM（+）	eCIM（+）
肺炎克雷伯菌	81	79	0	1	1	81	81	0
大肠埃希菌	12	3	9	0	0	3^①	12	9^②
产气肠杆菌	6	5	1	0	0	5^①	6	1^②
阴沟肠杆菌	4	1	3	0	0	1^①	4	3^②
产酸克雷伯菌	3	2	0	1	0	3	3	0
弗劳地枸橼酸杆菌	1	0	1	0	0	0	1	1
科泽枸橼酸杆菌	1	1	0	0	0	1	1	0
总计	108	91	14	2	1	94	108	14

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