doi:10.3969/j.issn.1673-8640.2023.01.012

Abstract

CLC Number:

R378.2

Figures/Tables 9

References 20

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[5]	李鑫, 杨继勇. 碳青霉烯类耐药肠杆菌科细菌分布与流行特征研究进展[J]. 中华微生物学和免疫学杂志, 2019, 39(4):306-310.
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基因名称	引物序列（5'~3'）	产物长度/bp	参考文献
bla_NDM
初筛引物：	F：TCACCGAGATTGCCGAGCGACTTG	146	[10]
	R：CCCAACTTTGGCCCGCTCAAGGTATT
检测引物：	F：GGGCAGTCGCTTCCAACGGT	475	[10]
	R：GTAGTGCTCAGTGTCGGCAT
bla_KPC	F：ATGTCACTGTATCGCCGTCT	893	[11]
	R：TTTTCAGAGCCTTACTGCCC
bla_CTX-M	F：CGCTTTATGCGCAGACGA	784	[12]
	R：GATTCTCGCCGCTGAAGC
bla_TEM	F：TCGGGGAAATGTGCG	972	[13]
	R：TGCTTAATCAGTGAG
bla_SHV	F：GCCTTTATCGGCCTTCACTCAAG	898	[13]
	R：TTAGCGTTGCCAGTGCTCGATCA

基因名称	引物序列（5'~3'）	产物长度/bp	参考文献
bla_NDM
初筛引物：	F：TCACCGAGATTGCCGAGCGACTTG	146	[10]
	R：CCCAACTTTGGCCCGCTCAAGGTATT
检测引物：	F：GGGCAGTCGCTTCCAACGGT	475	[10]
	R：GTAGTGCTCAGTGTCGGCAT
bla_KPC	F：ATGTCACTGTATCGCCGTCT	893	[11]
	R：TTTTCAGAGCCTTACTGCCC
bla_CTX-M	F：CGCTTTATGCGCAGACGA	784	[12]
	R：GATTCTCGCCGCTGAAGC
bla_TEM	F：TCGGGGAAATGTGCG	972	[13]
	R：TGCTTAATCAGTGAG
bla_SHV	F：GCCTTTATCGGCCTTCACTCAAG	898	[13]
	R：TTAGCGTTGCCAGTGCTCGATCA

抗菌药物	菌株总数/株	耐药菌株数/株	耐药率/ %
亚胺培南	198	177	89.4
厄他培南	173	164	94.8
头孢吡肟	204	195	95.6
头孢替坦	176	165	93.8
哌拉西林-他唑巴坦	202	183	90.6
氨苄西林-舒巴坦	159	158	99.4
庆大霉素	177	91	51.4
妥布霉素	177	84	47.5
阿米卡星	193	73	37.8
左氧氟沙星	203	164	80.8
环丙沙星	177	148	83.6
复方磺胺甲噁唑	200	107	53.5
呋喃妥因	176	121	68.8

抗菌药物	菌株总数/株	耐药菌株数/株	耐药率/ %
亚胺培南	198	177	89.4
厄他培南	173	164	94.8
头孢吡肟	204	195	95.6
头孢替坦	176	165	93.8
哌拉西林-他唑巴坦	202	183	90.6
氨苄西林-舒巴坦	159	158	99.4
庆大霉素	177	91	51.4
妥布霉素	177	84	47.5
阿米卡星	193	73	37.8
左氧氟沙星	203	164	80.8
环丙沙星	177	148	83.6
复方磺胺甲噁唑	200	107	53.5
呋喃妥因	176	121	68.8

菌株编号	细菌名称	产碳青霉烯酶基因型		ESBL基因
29W3	阴沟肠杆菌	bla_NDM-1		bla_CTX-M+bla_TEM
30Z23	大肠埃希菌	bla_NDM-5
32Z25	肺炎克雷伯菌	bla_NDM-1	bla_KPC-2	bla_TEM+bla_SHV
33Z26	肺炎克雷伯菌	bla_NDM-1		bla_SHV
15B116	肺炎克雷伯菌	bla_NDM-4		bla_SHV
68RM1	大肠埃希菌	bla_NDM-5
69RM2	肺炎克雷伯菌	bla_NDM-5	bla_KPC-2	bla_CTX-M+bla_TEM+bla_SHV
72RM6	大肠埃希菌	bla_NDM-4	bla_KPC-2	bla_CTX-M+bla_TEM+bla_SHV
73RM7	肺炎克雷伯菌	bla_NDM-4	bla_KPC-2	bla_CTX-M
74RM8	肺炎克雷伯菌	bla_NDM-4	bla_KPC-2	bla_CTX-M+bla_TEM+bla_SHV
75RM9	大肠埃希菌	bla_NDM-4	bla_KPC-2	bla_CTX-M+bla_TEM
76RM10	肺炎克雷伯菌	bla_NDM-5	bla_KPC-2	bla_CTX-M+bla_TEM
77RM11	大肠埃希菌	bla_NDM-5	bla_KPC-2	bla_CTX-M+bla_TEM
78RM13	大肠埃希菌	bla_NDM-4	bla_KPC-2
80RM16	大肠埃希菌	bla_NDM-1
28B106	肺炎克雷伯菌	bla_NDM-1		bla_TEM+bla_SHV
7B10①	肺炎克雷伯菌	bla_NDM-1		bla_CTX-M+bla_SHV
11B401①	肺炎克雷伯菌	bla_NDM-1		bla_SHV
11B401②	阴沟肠杆菌	bla_NDM-5
23B110	大肠埃希菌	bla_NDM-5	bla_KPC-2	bla_TEM
22B109	阴沟肠杆菌	bla_NDM-1		bla_TEM
2B111①	肺炎克雷伯菌	bla_NDM-5		bla_TEM
27B114	肺炎克雷伯菌	bla_NDM-1		bla_SHV
31B102	大肠埃希菌	bla_NDM-5		bla_CTX-M+bla_TEM
9B2	大肠埃希菌	bla_NDM-5	bla_KPC-2	bla_CTX-M+bla_TEM

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