Abstract:

Objective To investigate the sensitivity of carbapenem-resistant Enterobacteriaceae（CRE）with different resistant genotypes against tigecycline. Methods The isolates of Enterobacteriaceae against ertapenem were screened by modified Hodge test （MHT）,the diameters of tigecycline inhibition zones were determined by K-B method, and beta-lactamase gene of CRE was determined by polymerase chain reaction（PCR）. Results A total of 68 isolates of CRE were screened by MHT,and there were 48 positive isolates,accounting for 70.6%. In the 48 positive isolates, there were 40 isolates （83.3%） carrying hydrolyzed carbapenem antibiotics extended-spectrum beta-lactamase gene, including KPC,NDM-1,IMP-8 and IMP-4. KPC was the most,accounting for 67.5%. In the 68 isolates of CRE,54 isolates were sensitive,12 isolates were intermediary,2 isolates were resistant,and the total sensitivity was 97%. There was no statistical significance for the sensitivity of positive and negative isolates in the 68 isolates of CRE by MHT （χ²=3.599,P>0.05）. Conclusions Tigecycline for CRE exhibits excellent antibacterial activity. Antibacterial effect of tigecycline is not influenced by enzyme activity.

Key words: Carbapenem-resistant Enterobacteriaceae, Tigecycline, Sensitivity