Laboratory Medicine ›› 2022, Vol. 37 ›› Issue (10): 969-973.DOI: 10.3969/j.issn.1673-8640.2022.010.012
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YANG Xi, SONG Wenqi, DONG Fang(), MENG Qingying, ZHEN Jinghui, ZHOU Wei
Received:
2021-09-12
Revised:
2021-12-18
Online:
2022-10-30
Published:
2022-11-14
Contact:
DONG Fang
CLC Number:
YANG Xi, SONG Wenqi, DONG Fang, MENG Qingying, ZHEN Jinghui, ZHOU Wei. Application of Gram staining and MALDI-TOF MS in the rapid identification of pathogen kinds in children's positive blood culture[J]. Laboratory Medicine, 2022, 37(10): 969-973.
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URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2022.010.012
转种培养结果 | 菌株数/株 | 革兰染色镜检/株 | MALDI-TOF MS/株 |
---|---|---|---|
革兰阳性菌 | 95 | 93 | 77 |
凝固酶阴性葡萄球菌 | 58 | 58 | 44 |
金黄色葡萄球菌 | 10 | 10 | 10 |
肠球菌 | 10 | 9 | 9 |
链球菌 | 10 | 9 | 9 |
革兰阳性杆菌 | 3 | 3 | 3 |
藤黄微球菌 | 2 | 2 | 2 |
链霉菌属 | 1 | 1 | 0 |
缺陷乏氧菌 | 1 | 1 | 0 |
革兰阴性菌 | 63 | 61 | 58 |
肠杆菌科细菌 | 48 | 48 | 45 |
革兰阴性非发酵菌 | 13 | 11 | 12 |
奥斯陆莫拉菌 | 1 | 1 | 1 |
马耳他布鲁菌 | 1 | 1 | 0 |
酵母样真菌 | 4 | 4 | 3 |
白念珠菌 | 3 | 3 | 2 |
近平滑念珠菌 | 1 | 1 | 1 |
转种培养结果 | 菌株数/株 | 革兰染色镜检/株 | MALDI-TOF MS/株 |
---|---|---|---|
革兰阳性菌 | 95 | 93 | 77 |
凝固酶阴性葡萄球菌 | 58 | 58 | 44 |
金黄色葡萄球菌 | 10 | 10 | 10 |
肠球菌 | 10 | 9 | 9 |
链球菌 | 10 | 9 | 9 |
革兰阳性杆菌 | 3 | 3 | 3 |
藤黄微球菌 | 2 | 2 | 2 |
链霉菌属 | 1 | 1 | 0 |
缺陷乏氧菌 | 1 | 1 | 0 |
革兰阴性菌 | 63 | 61 | 58 |
肠杆菌科细菌 | 48 | 48 | 45 |
革兰阴性非发酵菌 | 13 | 11 | 12 |
奥斯陆莫拉菌 | 1 | 1 | 1 |
马耳他布鲁菌 | 1 | 1 | 0 |
酵母样真菌 | 4 | 4 | 3 |
白念珠菌 | 3 | 3 | 2 |
近平滑念珠菌 | 1 | 1 | 1 |
转种培养结果 | 革兰染色镜检结果 | MALDI-TOF MS快速鉴定结果 |
---|---|---|
黏质沙雷菌、粪肠球菌 | 革兰阳性球菌、革兰阴性杆菌 | 黏质沙雷菌 |
鲍曼不动杆菌、板崎肠杆菌 | 革兰阴性杆菌 | 鲍曼不动杆菌 |
阴沟肠杆菌、腐败西瓦菌 | 革兰阴性杆菌 | 阴沟肠杆菌 |
屎肠球菌、人葡萄球菌 | 革兰阳性球菌 | 屎肠球菌 |
转种培养结果 | 革兰染色镜检结果 | MALDI-TOF MS快速鉴定结果 |
---|---|---|
黏质沙雷菌、粪肠球菌 | 革兰阳性球菌、革兰阴性杆菌 | 黏质沙雷菌 |
鲍曼不动杆菌、板崎肠杆菌 | 革兰阴性杆菌 | 鲍曼不动杆菌 |
阴沟肠杆菌、腐败西瓦菌 | 革兰阴性杆菌 | 阴沟肠杆菌 |
屎肠球菌、人葡萄球菌 | 革兰阳性球菌 | 屎肠球菌 |
TTP | MALDI-TOF MS鉴定正确例数 | MALDI-TOF MS鉴定错误例数 | 合计 |
---|---|---|---|
≥21 h | 43 | 16 | 59 |
<21 h | 95 | 8 | 103 |
合计 | 138 | 24 | 162 |
TTP | MALDI-TOF MS鉴定正确例数 | MALDI-TOF MS鉴定错误例数 | 合计 |
---|---|---|---|
≥21 h | 43 | 16 | 59 |
<21 h | 95 | 8 | 103 |
合计 | 138 | 24 | 162 |
TTP | MALDI-TOF MS鉴定正确例数 | MALDI-TOF MS鉴定错误例数 | 合计 |
---|---|---|---|
≥21 h | 35 | 12 | 47 |
<21 h | 42 | 6 | 48 |
合计 | 77 | 18 | 95 |
TTP | MALDI-TOF MS鉴定正确例数 | MALDI-TOF MS鉴定错误例数 | 合计 |
---|---|---|---|
≥21 h | 35 | 12 | 47 |
<21 h | 42 | 6 | 48 |
合计 | 77 | 18 | 95 |
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