Virus spectrum of children's upper respiratory tract viral infection in Zhenjiang

doi:10.3969/j.issn.1673-8640.2021.04.013

Abstract

Abstract:

Objective Viral macrogenomics was used to study the composition of viral communities in nasal cavity of children with upper respiratory tract viral infection in Zhenjiang. Methods A total of 100 nasal mucosal secretion samples were collected from children with upper respiratory tract viral infection. Nucleic acid was extracted and sequenced by Miseque high-throughput sequencing. The viral community was analyzed by viral macrogenomics analysis platform. Results The viral community in the microenvironment of children's nasal mucosal secretion samples in Zhenjiang was mainly composed of adenoviridae (30.13%), picornaviridae (24.04%), coronaviridae (11.22%) and actinoviridae (10.58%), polyomaviridae (10.58%), paramyxoviridae (7.37%) and so on. The detected library viruses were mainly adenoviridae (30.13%) and picornaviridae (24.04%). Conclusions The virus spectrum of children's upper respiratory tract viral infection in Zhenjiang is various,which are mainly adenoviridae and picornaviridae. It provides a reference for the prevention and clinical treatment of children with upper respiratory tract viral infection.

Key words: Children, Upper respiratory tract, Viral mecrogenomics, High-throughput sequencing, Adenoviridae, Picornaviridae

CLC Number:

R446.1

WANG Yanan, ZHANG Wen. Virus spectrum of children's upper respiratory tract viral infection in Zhenjiang[J]. Laboratory Medicine, 2021, 36(4): 408-411.

Figures/Tables 2

References 12

[1]	WALKER C L F, RUDAN I, LIU L, et al. Global burden of childhood pneumonia and diarrhoea[J]. Lancet, 2013,381(9875):1405-1416. DOI URL PMID
[2]	NAIR H, SIMÕES E A, RUDAN I, et al. Global and regional burden of hospital admissions for severe acute lower respiratory infections in young children in 2010:a systematic analysis[J]. Lancet, 2013,381(9875):1380-1390. URL PMID
[3]	姚娟, 沈国松, 范丽红, 等. 6 089例住院儿童呼吸道感染常见病毒病原学检测分析[J]. 中华流行病学杂志, 2015,36(6):664-666.
[4]	BREIMAN R F, COSMAS L, NJENGA M, et al. Severe acute respiratory infection in children in a densely populated urban slum in Kenya,2007-2011[J]. BMC Infect Dis, 2015,15:95. DOI URL PMID
[5]	BELÁK S, KARLSSON O E, BLOMSTRÖM A L, et al. New viruses in veterinary medicine,detected by metagenomic approaches[J]. Vet Microbiol, 2013,165(1-2):95-101. DOI URL PMID
[6]	刘志坚. 孕期妇女阴道炎微环境内病毒群落组成的解析及新型HPV鉴定[D]. 江苏:江苏大学, 2017.
[7]	ZHANG W, YANG S, SHAN T, et al. Virome comparisons in wild-diseased and healthy captive giant pandas[J]. Microbiome, 2017,5(1):90. DOI URL PMID
[8]	胡天一. 儿童腺病毒感染及其引起的重症肺炎[J]. 医师在线, 2019,9(21):36-37.
[9]	KENNEDY J L, HEYMANN P W, PLATTS-MILLS T A. The role of allergy in severe asthma[J]. Clin Exp AIlergy, 2012,42(5):659-669.
[10]	PALLANSCH M, ROOS R. Fields virology[M]. 5th ed. Philadelphia:Lippincott Williams & Wilkins, 2007.
[11]	何彪, 涂长春. 病毒宏基因组学的研究现状及应用[J]. 畜牧兽医学报, 2012,43(12):1865-1870.
[12]	GEOGHEGAN J L, HOLMES E C. Evolutionary virology at 40[J]. Genetics, 2018,210(4):1151-1162. DOI URL PMID

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